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3, 2023

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Effect of T-2 toxin and zearalenone on the content of heat shock proteins in primary liver cell cultures

Authors: Andrey I. Samsonov, Alsu R. Makaeva, Rishat S. Mukhammadiev, Linar R. Valiullin, Zhanna R. Nasybullina

Federal State Budgetary Scientific Institution Federal Center for Toxicological, Radiation and Biological safety, Kazan, Russia


Contamination of food and feed with mycotoxins is of exceptional importance worldwide. In natural conditions, combined contamination of mycotoxins is more common. Toxin T-2 is one of the most dangerous agent of the group of trichothecene mycotoxins. At the cellular level, one of the main harmful effects of the T-2 toxin is the primary inhibition of protein synthesis due to binding of the peptidyltransferase enzyme and disruption of translation directed to the 60S ribosomal subunit. In addition, the presence of the toxin can lead to disorders at the genetic level, such as the induction of DNA fragmentation or DNA damage. Data on the effect of trichothecenes on the expression of the HSP70 heat shock protein gene are limited. In this regard, the aim of the study was to study the effect of T-2 toxin and zearalenone on the content of heat shock proteins in primary chicken liver cell cultures. To do this, the cell suspension was poured into glass vials of 2 cm3 each. T-2 toxin and zearalenone were introduced into vials in the form of an alcohol solution of 0.001 cm3 at doses of 0; 10; 100 and 1000 nM/m3. Vials with cellular suspension into which ethyl alcohol (96 vol. %) 0.001 cm3 was introduced were used as control. Subsequently, the vials were incubated at a temperature of 37 C for 24 hours. Then a dispersing solution was introduced in a volume of 0.2 cm3 per vial and incubated in a thermostat at 37 C for 20 minutes. The production of heat shock protein HSP70 was measured using ELISA. It was found that the introduction of zearalenone slightly changed the content of HSP70, the introduction of T-2 toxin, depending on the dose, caused an increase in the protein content up to 70 %, and the combined introduction of mycotoxins increased the protein content of heat shock by 180 %, which indicates a synergistic effect, however, the mechanisms of the effect require additional research.

Keywords: T-2 toxin, zearalenone, primary liver cell culture, heat shock proteins

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Study of acute toxicity of the liquid feed additive "Active Mix" VMG in experiments on rats.

Authors: Andrey N. Kulikov1, Alexander V. Shishkin2, Roman O. Vasiliev3, Marina S. Kulikova1, Ekaterina A. Mikheeva1

1Udmurt State Agrarian University Izhevsk, Russia

2Production company Izhsintez- Khimprom, Izhevsk, Russia

3St. Petersburg State University of Veterinary Medicine, St. Petersburg, Russia, secretary@spbguvm.ru

Abstract: When creating a liquid vitamin and mineral feed additive Active Mix VMG 500/600, a number of new solutions were used, aimed both at increasing the digestibility of vitamins and trace element compounds, and at reducing the toxicity of some of them. This feed additive consists of 2 parts (liquids) Active Mix VMG-500 and Active Mix VMG-600, which are given to animals separately. The article presents the results of a study of their acute toxicity during intragastric administration to laboratory rats, on the basis of which these liquids can be classified as low-hazard drugs (4th hazard class in accordance with GOST 12.1.007-76).
Keywords: trace elements, chelated complex compounds, vitamins, feed additive, acute toxicity, rats

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Assessment of mutagenic activity of a biological product based on lactic acid and propionic acid microorganisms in vivo

Authors: Rint S. Mukhammadiev, Rishat S. Mukhammadiev, Lenar R. Valiullin, Rifkat R. Musin, Anna M. Tremasova

Federal State Budgetary Scientific Institution Federal Center for Toxicological, Radiation and Biological safety, Kazan, Russia

Abstract: Lactic acid and propionic acid bacteria are widely used in various fields of industry, veterinary medicine, food and pharmaceutical industries. In order to obtain high-quality feed necessary to create optimal conditions for the development of normal microflora of the gastrointestinal tract of farm animals and birds and, thus, to increase the number of livestock, it is relevant to develop drugs based on a combination of these bacteria to improve the technology of canning and silage feed. At the same time, according to the World Health Organization, the European Food Safety Agency, drugs based on such consortia should not only be effective, but also safe. The mutagenic activity of a biological product based on lactic acid and propionic acid microorganisms was evaluated in vivo. The mutagenic activity of the drug developed by us based on strains of lactic acid and propionic acid microorganisms using the method of accounting for chromosome aberrations in mouse bone marrow cells did not reveal a statistically significant increase in the number of cells with structural chromosome disorders compared to the negative control group. The micronucleus test also showed no increase in the number of micronuclear erythrocytes in mice compared with the negative control group. The data obtained by the method of accounting for chromosome aberrations in mouse bone marrow cells are confirmed by the results obtained by the method of counting micronuclei in mammalian cells and indicate the safety of a biological product based on lactic and propionic acid microorganisms, as well as the possibility of its further use in agriculture and veterinary medicine.
Keywords: biological product, lactic acid, propionic acid bacteria, mutagenicity, in vivo

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Reproductive qualities of repair pigs against the background of the use of immunotropic drugs

Authors: Alyona V. Kovalenko, Dmitry A. Nikitin, Vladimir G. Semenov, Lyubov P. Gladkikh, Lyudmila A. Semenova

Chuvash State Agrarian University, Cheboksary, Russia

Abstract: The purpose of this work is to realize the biopotential of reproductive qualities of repair pigs by immunocorrection of the body with PigStim-V and PigStim-F. For the experiment, 3 groups of 10 heads each were formed from 30 suckling pigs (pigs). Pigs of the 1st experimental group were injected intramuscularly with the immunotropic drug PigStim-V at a dose of 1 ml per head three times, on the 15th, 20th and 25th days of life. Pigs of the 2nd experimental group were injected with the immunotropic drug PigStim-F according to a similar scheme. The control group pigs were not injected with immunotropic drugs. Insemination of pigs was carried out in the 4th sexual hunt, at the age of 7.5-8.0 months and the optimal live weight. The positive effect of the immunotropic drugs PigStim-V and PigStim-F on the reproductive qualities of repair pigs was revealed. In animals of the 1st and 2nd experimental groups, there is a decrease in the age of manifestation of the first sexual hunt by 4.2 and 5.4 days, and the age of the first insemination by 4.0 and 5.8 days, and its fruitfulness increases. Against the background of immunocorrection of the body, the rate of multiple births increased by 6.78 and 10.17 %, and stillbirth decreased by 33.3 %. Immunotropic drugs helped to reduce the incidence of postpartum diseases in sows, such as metritis-mastitis-agalactia syndrome and increased the effectiveness of therapeutic measures to 100%. Against the background of the use of PigStim-V and PigStim-F for repair pigs, the safety of piglets obtained from them increased by 1.80 and 1.85 %, and the live weight at weaning was higher than the control values by 0.12 and 0.10 kg. The duration of the period from weaning to the onset of the next sexual hunt in sows of the 1st and 2nd experimental groups decreased by an average of 0.4 and 0.2 days.
Keywords: repair pigs, immunotropic drugs Pigs team-V and Pigs team-F, multiple pregnancy, metritis-mastitis-agalactia syndrome, preservation, live weight at weaning, nest weight, the period from weaning to insemination

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In vitro characteristics of natural sorbents to a pesticide of the neonicotinoid class

Authors: Nailya N. Mishina, Damir V. Aleev, Guzaliya R. Yamalova, Kadria F. Khalikova, Gulnara G. Galyautdinova

Federal State Budgetary Scientific Institution Federal Center for Toxicological, Radiation and Biological safety, Kazan, Russia

Abstract: Contamination of feed with neonicotinoids in natural conditions is possible if plant treatment regimes are not observed, during harvesting, during storage and processing. While, getting into the food chain, the pesticide poses a serious threat to animal and human health. One of the ways to reduce the negative impact of various toxicants on the body is the use of additives with high sorption properties. In order to study the sorption properties, we selected zeolite and shungite. Zeolite is a widespread useful mineral that has sorption properties to various substances, enriches the body with trace elements and improves metabolism. Shungite is a carbon-containing rock with high mechanical strength, good filtering ability and the ability to sorb organic and mineral substances. Once in the body, shungite has an analgesic effect and bactericidal properties. The aim of our work was to study the features of the sorption of imidacloprid with sorbents shungite and zeolite and their combinations in vitro. As a result of the conducted studies, it was found that the sorbents showed the greatest adsorption capacity for imidacloprid in the following sequence: shungite 30 %, zeolite/shungite (30/70) 26 %, zeolite/shungite (50/50) 22 %, zeolite/shungite (70/30) 10.2 %, zeolite 6.6 %. Thus, sorbents shungite and its combination with zeolite (where the percentage of shungite is greater) have pronounced sorption properties with respect to imidacloprid, and are promising for further study in in vitro experiments with other neonictinoids.
Keywords: imidacloprid, adsorbent, zeolite, shungite, adsorption, desorption

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Monitoring of veterinary safety of areas of extraction (catch) of aquatic biological resources as a mechanism for ensuring food and biological safety of the territory of the Russian Federation (2019-2021)

Authors: Ilyas Faritovich Adiatulin1, Vasily Ivanovich Belousov2, 3, Serikbol Beilzhanovich Bazarbaev3 1natsrybbesafety, Moscow, Russia

1Natsrybbesafety, Moscow, Russia

2The All-Russian Scientific Research Institute for Animal Protection, Vladimir, Russia

3Moscow State Academy of Veterinary Medicine and Biotechnology named after K. I. Scriabin, Moscow, Russia

Abstract: The article presents the results of monitoring studies of veterinary safety of the catch of aquatic biological resources in the territory of the Russian Federation for the period from 2019 to 2021. A total of 84,216 samples of fish and fish products were examined during the study period, 70% of the samples were taken in marine regions and 30% in inland waters. At the same time, in 119 districts the catches of aquatic biological resources met the requirements of their safety, and in 96 districts they did not meet. The largest number of cases of parasitic infection of aquatic biological resources (637 cases 81.3%) is observed in the marine areas of the Far Eastern Fisheries Basin, of which 318 (91.9%) are in the Sea of Okhotsk. Helminths, histamine, mercury, cadmium, arsenic, organochlorine compounds (HOC) were detected in the samples studied. Recommendations are given on improving the methods of control of fish and fish products produced on the territory of the Russian Federation.
Keywords: laboratory tests, fish, catches, pathogens, pollutant

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