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  Infectious pathology
   
Title:

Biological properties of bacteria of the genus Moraxella isolated in the Republic of Kazakhstan

   
Authors: Rano S. Sattarova 1, Flyura A. Bakiyeva 2, Karlygash . Boranbayeva 3, Aidana . Askarova 4, Gennady N. Spiridonov 5
   
Address:

1, 2 Kazakh Scientific Research Veterinary Institute LLP, Department of epizootic monitoring and risk assessment of bacterial animal diseases, Almaty, Kazakhstan, ranosaitomarovna@gmail.ru

3, 4 Kazakh National Agrarian Research University, Department of Clinical Veterinary Medicine, Almaty, Kazakhstan, 17karla@mail.ru

5 Federal Center for Toxicological, Radiation and Biological Safety, Laboratory of Bacterial Animal Pathologies, Department of Bacteriology, Kazan, Russia, vnivi@mail.ru

   
Abstract: . The article presents the results of studying the biological properties of bacteria of the genus Moraxella, isolated from the of affected eyes cattle, kept in economic entities of the Republic of Kazakhstan. To bacteriological examination 339 biomaterial samples were subjected. The biological properties of two epizootic isolates of bacteria of the genus Moraxella, isolated from sick animals, as well as reference strains of Moraxella bovis ATCC 17948 and Moraxella bovoculi BAA 1259 obtained from LGC Standards Sp.z. o.o." (Poland). The obtained results indicate that the bacteria Moraxella bovis and Moraxella bovoculi, are causative agents of Pinkeye in cattle, have hemolytic properties, hyaluronidase activity in vivo, do not ferment sugar, are catalase-negative, and do not form indole. Bacteria Moraxella bovis are diplobacilli, 1.5-2.0 m long and 0.5-1.0 m wide with rounded ends, arranged in pairs, in the form of short chains or singly. Old cultures and cultures after 48-72 hours of incubation progressively become polymorphic, showing variability in cell size and shape. Moraxella bovoculi bacteria are gram-negative diplococci with a cell diameter of 0.7-1.3 m. Study of deaminase activity of Moraxella cultures, shows positive results in Moraxella bovoculi bacteria: in the reference strain BAA 1259 and epizootic strain 2 FarmAgro. Reference and field strains of Moraxella bovis ATCC 17948 don t hove deaminase activity. Bacteria of the genus Moraxella were pathogenic for white mice when administered intraperitoneally. The results of our research should be taken into account when developing therapeutic and preventive measures.
   
Keywords: cattle, Pinkeye of cattle, infectious keratoconjunctivitis, Moraxella, biological properties
   
References:

1.         Vysotsky, A. E. Handbook of bacteriological research methods in veterinary medicine / A. E. Vysotsky, Z. N. Baranovskaya. URL: http://www. studmed.rubaranovskaya-znbakteriologicheskimv (accessed: June 11, 2022).

2.         Lebedev, A. V. Private veterinary surgery / A. V. Lebedev, V. A. Chervanev // Moscow : Kolos, 1997. P. 496.

3.         Maichuk, Yu. F. New in the epidemiology and pharmacotherapy of eye infections / Yu. F. Maychuk // Clinical ophthalmology. 2000. Vol. 1. P. 4851.

4.         Guidelines for the diagnosis, treatment and specific prevention of infectious bovine keratoconjunctivitis caused by the bacteria Moraxella bovis and Moraxella bovoculi / G. N. Spiridonov, Kh. Z. Gaffarov, A. I. Nikitin [et al.]. Moscow : Rosinformagrotech, 2017. P. 36.

5.         Pinkeye of cattlein in Kazakhstan / N. P. Ivanov, A. A. Sultanov, F. A. Bakieva [et al.] // Izvestiya NAN RK. Seriya Agrarnykh Nauk. 2016. No 5 (35). . 2029.

6.         Sarimbekova, S. N. Pathogenicity factors of bacteria found (inhabited) in goat milk : Cand. ... master of tech. Sciences, 2014. P. 74.

7.    Subbotin, V. V. Biological properties of the isolated Moraxella bovis cultures / V. V. Subbotin, D. V. Karaichentsev, V. N. Karaichentsev // Veterinary Pathology. 2014. No 2. P. 3134.

8.         Moraxella bovoculi strain CX-Ch6 No. DEP of the causative agent of infectious bovine keratoconjunctivitis. / G. N. Spiridonov, L. Sh. Dupleva, A. G. Spiridonov [et al.] // Uchenye zapiski KGAVM im. N.E. Bauman. 2017. Vol. 232 (No 4). P. 140143.

9.    Angelos, J. A. Infectious bovine keratoconjunctivitis (pinkeye) / J. A. Angelos // Vet. Clin N Am Food Anim Pract. 2015. No 31. . 6179.

10.  Brown, J. F. Relationship of feeding activity of face fly (Musca autumnalis DeGeer) toproduction of keratoconjunctivitis in calves. / J. F. Brown, Jr. Adkins // Am. J. Vet. Res. 1972. No 33. P. 25512555.

11.  Gabriela, C. P. Moraxella bovis pathogenicity: An update / C. P. Gabriela, J. C. Carfagnini, L. Minatel // Comparative Immunology, Microbiology and Infectious Diseases. 2008. No 31. P. 449458. URL: https://doi.org/10.1016/j.cimid.2008.04.001 (https://www.sciencedirect.com/science/article/pii/S0147957108000155  (accessed: 11.06.2022).

12.      Krafsur, E. S. Bovine thelaziasis in Iowa / E. S. Krafsur, C. J. Church // J Parasitol. 1985. No 71(3): .279-86. https://www.ncbi.nlm.nih.gov/pubmed /4040164) (accessed: 11.06.2022).

13.      Ladouceur, C. A. Eye worms in cattle in Indiana / C. A. Ladouceur, K. R. Kazacos // J Am Vet Med Assoc. 1981. 178 (4): 385-7. URL: https: // www.ncbi.nlm.nih.gov/pubmed/7195389 (accessed: 11.06.2022).

14.      Moraxella bovoculi sp. nov., isolated from calves with infectious bovine keratoconjunctivitis / J. A. Angelos, P. Q. Spinks, L. M Ball, L. W. George // Int J Syst Evol Microbiol. 2007. No 57. . 789795.

15.      Pathogenesis of Bacterial Infections in Animals. Fourth Edition / Carlton LGyles, John FPrescott, Glenn Songer, Charles OThoen. 2010. P. 469481.

16.      Schnee, C. Point prevalence of infection with Mycoplasma bovoculi and Moraxella spp. in cattle at different stages of infectious bovine keratoconjunctivitis / C. Schnee, M. Heller, E. Schubert // Vet J. 2015. No 203 (1). . 92.

17.      Transmission of Moraxella bovis from Blood Agar Cultures to Hereford Cattle by Face Flies (Diptera: Muscidae) / J. J. Arends, R. E. Wright, P. B. Barto, K. S. Lusby // J. Econ. Entomol. 1984. No 77. P. 394398.

18.      The role of face flies in an episode of infectious bovine keratoconjunctivitis / R. R. Gerhardt, J. W. Allen, W. H. Greene, P. C. Smith // J. Am. Vet. Med. Assoc. 1982. No 180. P. 156159.

19.      Whittier, W. Pinkeye in Beef Cattle / W. Whittier, John Currin, Nancy Currin // Virginia Cooperative Extension Publications. URL: https: // ext.vt.eduindex.html. Published: May 01 2009.

   
   
Title:

Study of the protective effect of prophylactic complexes on the ultrastructure of rabbits hepatocytes with combined mycotoxicosis

   
Authors: Evgenya Yu. Tarasova , Gleb S. Kashevarov, Vadim R. Saitov, Ksenia V. Perfilova, Ainur I. Yarullin
   
Address:

Federal State Budgetary Scientific Institution Federal Center for toxicological, radiation, and biological safety, Kazan, Russia.

   
Abstract: Mycotoxins have a significant impact on the health and productivity of animals, as well as the quality and safety of food and feed, which leads to the need for research on a detailed study of the mechanism of action and the search for effective means of preventing mycotoxicoses. The aim of the work was to study the effect of prophylactic complexes developed at the Federal State Budgetary Scientific Institution Federal Center for Toxicological, Radiation and Biological Safety on the liver ultrastructure and morphometric characteristics of rabbit mitochondria under combined exposure to mycotoxins (T-2 toxin, aflatoxin B1, zearalenone). Studies to determine the protective effect of prophylactic complexes in mixed mycotoxicosis on the ultrastructure of hepatocytes were performed on 96 chinchilla rabbits, divided into eight groups of twelve individuals each. It has been shown that with simultaneous intoxication with high doses of mycotoxins, changes in the ultrastructure of the nuclei are observed in hepatocytes: nuclei with uncharacteristic outlines or atypical shapes appear, when cavities and irregularities appear in the outer contour of some nuclei, but the structure of the perinuclear space does not change, reorientation and even chromatin disintegration, morphometric characteristics of mitochondria change (perimeter, area and diameter). At the same time, the most pronounced protective effect on the studied cellular structures was shown by the prophylactic complex No 3, including halloysite, methionine, β-glucans, milk thistle meal.
   
Keywords: rabbits, mycotoxins, prevention, liver, ultrastructure, hepatocytes, mitochondria, morphometry
   
References:

1.    Baskova, E. Yu. The use of enterosorbents based on nanotechnologies for the control of animal mycotoxicoses / E. Yu. Baskova // Scientific notes of the Kazan State Academy of Veterinary Medicine named after N. E. Bauman. 2008. Vol. 192. . 234.

2.    Valiev, A. R. Immunosuppression in the pathogenesis of T-2 mycotoxicosis and its pharmacocorrection / A. R. Valiev, E. I. Semyonov, F. G. Akhmetov // The veterinarian. 2011. No 2. P. 46.

3.    Gulyushin, S. Yu. Donors of methyl groups - promising means for the prevention of chronic mycotoxicoses / S. Yu. Gulyushin, R. A. Zernov // Agricultural biology. 2011. No 2. P. 2131.

4.    Guidelines for electron microscopic studies of biological objects / A. V. Ivanov, A. A. Ivanov, A. N. Chernov [et al.]. Kazan : Rosinformagrotech, 2011. 67 p.

5.    Nanotubes of halloysite as a new effective tool for combating mycotoxicoses / E. Yu. Tarasova, E. I. Semenov, L. E. Matrosova [et al.] // Scientific Life. 2020. Vol. 15, No 4 (104). . 561571.

6.    Evaluation of feed toxicity by regions of the Russian Federation / S. A. Semenova, R. M. Potekhina, E. I. Semenov [et al.] // Scientific notes of the Kazan State Academy of Veterinary Medicine named after N. E. Bauman. 2015. Vol. 224, No 4. . 196199.

7.    Papunidi, E. K. Veterinary and sanitary examination of sheep meat in acute and subacute T-2 mycotoxicosis against the background of the use of drugs / E. K. Papunidi, M. Ya. Tremasov, E. Yu. Tarasova // The veterinarian. 2010. No 2. P. 2123.

8.    A case of avian mycosis caused by a toxigenic isolate of Fusarium proliferatum / R. M. Potekhina, L. E. Matrosova, E. Yu. Tarasova [et al.] // Bulletin of the Mari State University. Series: Agricultural sciences. Economic sciences. 2019. Vol. 5, No 3 (19). . 316322.

9.    Comparative evaluation of the adsorbing activity of yeast in relation to mycotoxins / E. I. Semenov, L. E. Matrosova, E. Yu. Tarasova [et al.] // Bulletin of the Kazan Technological University. 2013. Vol. 16, No 10. . 195197.

10.               Tarasova, E. Yu. Study of the sorption activity of halloysite nanotubes in relation to zearalenone and ochratoxin A / E. Yu. Tarasova // Bulletin of the Mari State University. Series: Agricultural sciences. Economic sciences. 2021. Vol. 7, No 1 (25). P. 6470.

11.               Tarasova, E.Yu. Finding means for the treatment of animals with T-2 mycotoxicosis : specialty 06.02.03 Veterinary pharmacology with toxicology, 06.02.02 Veterinary microbiology, virology, epizootology, mycology with mycotoxicology and immunology : dissertation for the degree of candidate of biological sciences. Kazan, 2010. 209 p.

12.               Tremasov, M. Ya. Experience in the use of probiotics in mycotoxicoses / M. Ya. Tremasov, L. E. Matrosova, E. Yu. Tarasova // Veterinary Bulletin. 2009. 3 (50). P. 3841.

13.              Cytotoxic activity of T-2 toxin to transplanted cell cultures of the lung epithelium of the cattle embryo / I. I. Idiyatov, L. R. Valiullin, V. V. Biryulya [et al.] // Genes and Cells. 2017. Vol. 12, No 1. . 4146.

14.               Experimental combined mycotoxicosis of pigs against the background of an infectious load / E. I. Semenov, L. E. Matrosova, S. A. Tanaseva [et al.] // Agricultural Biology. 2022. Vol. 57, No 2. . 371383.

15.               The effectiveness of adsorbents in combined mycotoxicosis of broiler chickens / S. A. Tanaseva, E. Yu. Tarasova, L. E. Matrosova [et al.] // International Veterinary Bulletin. 2020. No 4. P. 5056.

16.               Co-Occurrence of Mycotoxins in Cereal-Based Feed and Food / R. Palumbo, A. Crisci, A. Venâncio [et al.] // Microorganisms. 2020. No 8. . 74.

17.               Enterosorbent efficiency mineral attenuation during pig mycotoxicosis / L. . Matrosova, N. N. Mishina, S. A. Tanaseva [et al.] // International Journal of Mechanical and Production Engineering Research and Development. 2020. Vol. 10. P. 1851.

18.               Fiji: an open-source platform for biological-image analysis / J. Schindelin, I. Arganda-Carreras, E. Frise [et al.] // Nature methods. 2012. Vol. 9, No 7. . 676682.

19.               Recent advances in assessing the effects of mycotoxins using animal models / A. F. Furian,  M. R. Fighera, L. F. Royes [et al.] // Current Opinion in Food Science. 2022. Vol. 47. 100874.

20.               Zeolite, hepatoprotector and probiotic for aflatoxicosis in pigs international / L. . Matrosova, S. A. Tanaseva, E. Y. Tarasova [et al.] // International Journal of Mechanical and Production Engineering Research and Development. 2020. Vol. 10. P. 7053.

   
   
Title:

Comprehensive treatment wound infections in animals

   
Authors: Rashid M. Aslanov1, Alizade S. Gasanov2, Zulfiyat M. Zukhrabova2, Gulam R. Allahverdiyev, Vusalya V. Sardarly, Rumiya A. Suleymanova
   
Address:

1 Federal State Budgetary Scientific Institution Federal Center for toxicological, radiation, and biological safety, Kazan, Russia

2 Kazan State Academy of Veterinary Medicine named after N.E. Bauman, Kazan, Russia

3 Azərbaycan Dövlət Aqrar Universiteti, Gyandzha, Azerbaijan

   
Abstract: The article presents experimental data on the results of the action of the natural sorbent zeolite in combination with an antimicrobial drug in the treatment of wound infections in animals. The natural sorbent zeolite was used in various variants in the studies: namely, in the 1st scheme - mechanical cleaning, zeolite, levomekol, in the 2nd scheme - mechanical cleaning, zeolite and in the 3rd scheme - mechanical cleaning, tetracycline ointment. According to the results of the performed studies, it was found that the maximum effect of treatment was shown by the natural sorbent zeolite when used in combination with mechanical cleaning of the wound surface and antibacterial ointment levomekol.
   
Keywords: adsorption, natural sorbent, zeolite, antibacterial drugs, animals, wound, infection, treatment.
   
References:

1.    Lutsevich, O. E. Modern view on pathophysiology and treatment of purulent wounds / O. E. Lutsevich, O. B. Tamrazova, A. Yu. Shikunova //Surgery. 2011. No 5. . 7277

2.    Maryin, E. M. Natural sorbents in the treatment of purulent wounds in animals / E. M. Maryin, V. A. Ermalaev, O. I. Maryin. Ulyanovsk, 2010. 140 .

3.    Panichev, A. M. The use of zeolites in medicine / A. M. Panichev, Yu. V. Kulakov, A. N. Gulkov // Pacific Medical journal. 2003. No 4. . 2124

4.    Persaeva, N. S. Etiopathogenetic therapy of skin-muscle wounds in rabbits and sheep / N. S. Persaeva, F. N.Chehodaridi, M. S.Gugkaeva // Izvestiya Gorsky State Agrarian University. Vladikavkaz, 2018. Vol. 55, . 2. . 97104

5.    Peshikov, O. V. Development of surgery and treatment of wounds: a textbook. / O. V. Peshikov, A. Yu. Markina. Chelyabinsk : YUUGMU, 2016. P. 79.

6.    Chehodaridi, F. N. Complex therapy of accidental infectious wounds in calves / F. N. Chehodaridi, N. S. Persaeva, M. S. Gugkaeva // Hippology and veterinary medicine. 2016. N 1 (19). P. 110-115.

7.    Galimzyanov, I. G. Inflammation as a local protective and adaptive reaction of the body to injury / I. G. Galimzyanov, A. N. Valeeva, . . Sergeev, O. I. Shorkina. Kazan, 2020. 86 p.

8.    Shorkina, O. I. The effect of Anilkam ointment on the wound process in sheep / O. I. Shorkina, A. N. Valeeva // Scientific notes of the Kazan State Academy of Veterinary Medicine named after N.E. Bauman. 2009. Vol. 197. . 408412.

9.    Zukhrabov, M. G. Toxicological evaluation of the drug with parenteral administration / M. G. Zukhrabov, O. A. Gracheva, Z. M. Zukhrabova // Izvestiya Orenburg State Agrarian University. 2018. N 5 (73). P. 186189

1.     Galimzyanov, I. G. Fibrous ion-exchange tissue for the treatment of infected wounds / I. G. Galimzyanov // Scientific notes of the Kazan State Academy of Veterinary Medicine. 2012. Vol. 210. . 5356.

   
   
Title:

Cultural and morphological properties of B. abortus strain 82-Tr

   
Authors: Maxim A. Kosarev, Yana A. Bogova, Gulnara M. Safina, Liliya A. Tukhvatullina
   
Address:

Federal State Budgetary Scientific Institution Federal Center for toxicological, radiation, and biological safety, Department of Bacteriology, Laboratory of Bacterial Zooanthroponoses, Kazan, Russia,

   
Abstract: The present paper shows study of cultural and morphological properties of the culture of B. abortus strain 82-Tr. It was found that the culture of strain 82-Tr grew well on liver media at 37 C, forming H2S. Colonies of B. abortus grew on a medium containing tetracycline. Their color according to White-Wilson (all dilutions of thionine and fuchsin) appears purple with yellow stripes extending radially from the center. Agglutination reaction on glass showed a positive result with brucellosis S-, R- and monoreceptor A sera and wasnt lysed by the Tb- phage and had positive tests for dissociation.
   
Keywords: brucellosis, B. abortus, cultural and morphological properties, antibiotic-resistant strain
   
References:

1. Gordienko, L. N. The effectiveness of the differential test in the diagnosis of brucellosis of reindeer / L. N. Gordienko, A. N. Novikov, E. V. Kulikova // Veterinary medicine. 2020. No 11. P. 710.

2. GOST 33675-2015. Laboratory diagnosis of brucellosis. Bacteriological methods. General provisions = Small craft infrastructure. General provisions : national standard of the Russian Federation : official publication : approved and put into effect by the order of the Federal Agency for Technical Regulation and Metrology dated November 24, 2015 : introduced for the first time : date of introduction January 01, 2017 / developed by: FSBI VGNKI. Moscow : Standartinform, 2016. 17 p.

3. Zhilina, N. Ya. Brucellosis in the Russian function / N. Ya. Zhilina // Sanitary doctor. 2007. No 6. P. 14-21.

4. Kosarev, M. A. Differential serological diagnosis of brucellosis in cattle vaccinated from strain 82, and its significance in the general system of control measures with this disease / M.A. Kosarev, A.M. Fomin, G.M. Safina [et al.] // Veterinary doctor. 2019. No 5. P.2328.

5. MUC 3.1.7.3402-16. Epidemiological surveillance and laboratory diagnostics of brucellosis. General provisions = Small craft infrastructure. General provisions : national standard of the Russian Federation : official publication : approved and put into effect on November 10, 2016 / developed by : The Federal Service for Supervision of Consumer Rights Protection and Human Well-being; the Federal State Health Institution "Stavropol Research Anti-Plague Institute" of Rospotrebnadzor. Moscow : Federal Center of Hygiene and Epidemiology of Rospotrebnadzor, 2017. 61 p.

6. Salmakova, A. V. Cultural-morphological and biochemical properties of vaccine strains of brucella / A. V. Salmakova // Mater. scientific. conf. of young scientists and specialists Actual problems of veterinary medicine, June 29, 2007 Kazan: Federal State University FCTRB-VNIVI, 2007. P. 131134.

7. Salmakov, K. M. An improved medical system for the prevention and elimination of brucellosis of cattle with the use of live vaccines from strains of weakly agglutinogenic B. abortus 82 and inagglutinogenic B. abortus R-1096 / K. M. Salmakov, A. M. Fomin, A. V. Ivanov [et al.] // Scientific notes of the Kazan State Academy of Veterinary Medicine. N. E. Bauman. 2012. . 211. P. 130134.

8. Blasco, J. Brucellosis vaccines and vaccine candidates / J. Blasco, E. Moreno, I. Moriyón // Veterinary Vaccines for Developing Countries. 2016. Vol. 5.

9. Goodwin, Z. Brucellosis vaccines for livestock / Z. Goodwin, D. Pascual // Veterinary Immunology and Immunopathology. 2016. Vol. 181. P. 5158.

 

   
   
Title:

Microbiocenosis of the genital tract in cows with acute postpartum endometritis

   
Authors: Ruslan R. Khamidullin, Anna M. Tremasova, Ilgiz I. Idiyatov, Nail I. hammadov, Yuri M. Tremasov
   
Address:

Federal Center for Toxicological, Radiation, and Biological Safety, Kazan, Russia

   
Abstract:

1. Bagmanov, M. A. Microflora of the uterus of cows after pathological childbirth / M. A. Bagmanov, R. N. Safiullov // Russian Veterinary Journal. 2007. Special issue. . 12.

2. Brekhov, T. P. The importance of monitoring the antibacterial activity of drugs in the treatment of cows with endometritis / T. P. Brekhov, V. V. Filin, L. I. Efanova // Actual problems of veterinary pharmacology, toxicology and pharmacy : at. 3 Congress of Pharmacologists and toxicologists of Russia. St.-Petersburg, 2011. 76 p.

3. Vakhitov, I. I. Study of the etiology and spread of postpartum endometritis in cows on farms of the Republic of Tatarstan / I. I. Vakhitov, M. A. Bagmanov, R. K. Shaev // Scientific notes of the Kazan State Academy of Veterinary Medicine named after N.E. Bauman. 2012. Vol. 211. P. 229233.

4. Voitenko, L. G. The influence of microbial factor on the occurrence of latent endometritis in cows / L. G. Voitenko, T. I. Lapina, I. A. Golovan // Proceedings of the Samara State Agricultural Academy. 2015. No 1. . 2325.

5. Griga, E. N. Causes, ways and methods of elimination of infertility of cows in the Stavropol territory / E. N. Griga // Bulletin of Veterinary Medicine. 2000. No 16 (2). P. 57-59.

6. Griga, E. N. Postpartum pathology of cows (etiology, diagnosis, therapy and prevention): specialty 16.00.07 Veterinary obstetrics and biotechnology of animal reproduction : abstract of dis. Dr. veterinarian. Sciences / Grig Eduard Nikolaevich ; Stavrop. state agrarian un-t. Stavropol, 2003. 49 p.

7. Grigorieva, I. E. The results of the use of electropuncture for the prevention of uterine diseases in cows after childbirth / T. E. Grigorieva, S. G. Kondruchina, L. A. Trifonova // Modern problems of veterinary obstetrics and animal reproduction biotechnology : at. International Scientific and Practical Conference, dedicated. To the 85th anniversary of the birth of Professor G. A. Cheremisinov and the 50th anniversary of the establishment of the Voronezh School of Veterinary Obstetricians. Voronezh : Istoki. 2012. . 162166.

8. Davletshina, G. K. Microbiological methods : textbook. manual / G. K. Davletshina, M. M. Tuigunov, Y. Z. Gabidullin. Ufa : Publishing House of the BSMU of the Ministry of Health of Russia, 2018. . 2835

9. Degtyareva, S. S. Species composition and sensitivity of microorganisms from cervical flushes in postpartum purulent-catarrhal endometritis in cows / S. S. Degtyareva, I. S. Koba // Actual problems of veterinary medicine in modern conditions : at. international scientific and practical conf., dedicated. The 60th anniversary of the Wildebeest of the Krasnodar NIVI. Krasnodar, 2006. . 336338.

10. Ivanyuk, V. P. Etiopathogenesis of postpartum endometritis in cows / V. P. Ivanyuk, G. N. Bobkova // Proceedings of the Orenburg State Agrarian University. 2022. No 2 (94). P. 191195.

11. Leonov, K. V. Possibility of correction of reproductive function in cows in various states of natural resistance: specialty 16.00.03 Veterinary microbiology, virology, epizootology, mycology with mitotoxicology and immunology, 16.00.07 Veterinary obstetrics and biotechnology of animal reproduction : abstract dis. ... Dr. veterinarian. Sciences / Leonov Konstantin Vasilyevich ; Moscow state acad. veterinarian. medicine and biotechnology them. K.I. Scriabin. Moscow, 2006. 37 p.

12. Nikitin, V. Ya. The main causes of infertility in the Stavropol Territory and measures to prevent it / V. Ya. Nikitin // Scientific Tr. Stavropol : Stavropol SCI, 1972. . 35.

13. Tremasov, Yu. M. Pharmaco-toxicological and biological evaluation of the drug DS-2 and its use in endometritis in animals: specialty 16.00.04 Veterinary pharmacology with toxicology, 16.00.03 Veterinary microbiology, virology, epizootology, mycology with mitotoxicology and immunology :   dis. ... cand. biol. Sciences / Tremasov Yuri Mikhailovich. Kazan, 2009. 139 p.

14. Turchenko, A. N. Development and improvement of therapeutic and preventive measures for acute postpartum endometritis in cows: specialty 16.00.07 Veterinary obstetrics and biotechnology of animal reproduction : dis. ... Dr. veterinarian. Sciences / Turchenko Alexey Nikolaevich. Voronezh, 1999. 385 p.

15. Chehodaridi, F. N. Etiopathogenetic therapy of purulent-catarrhal endometritis in cows / F. N. Chehodaridi, L. A. Mugnieva // News of the Gorsky State Agrarian University. 2015. Vol. 52, No 2. . 111115.

16. Defining postpartum uterine disease and the mechanisms of infection and immunity in the female reproductive tract in cattle / I. M. Sheldon, J. Cronin, L. Goetze [et al.] // Biol Reprod. 2009. No 81 (6). . 10251032.

   
   
Title:

Monitoring of veterinary safety of areas of extraction (catch) of aquatic biological resources as a mechanism for ensuring food and biological safety of the Territory of the Russian Federation (2017-2018)

   
Authors: Ilyas F. Adiatulin 1, Vasily I. Belousov 2, 3, Serikbol B.Bazarbayev 3
   
Address:

1 National Fish Safety, Moscow, Russia

2 The All-Russian Scientific Research Institute for Animal Protection, Vladimir, Russia

3 Moscow State Academy of Veterinary Medicine and Biotechnology named after K.I. Scriabin, Moscow, Russia

   
Abstract: The article presents the results of monitoring studies of veterinary safety of catching aquatic biological resources on the territory of the Russian Federation for the period 2017 to 2018. A total of 447784 samples of fish and fish products were examined during the study period. Histamine, benz(a)pyrene, mercury, cadmium, arsenic, and XOC were detected in the samples studied. Recommendations are given on improving the methods of control of fish and fish products in the areas of extraction (catch) of aquatic biological resources on the territory of the Russian Federation [4].
   
Keywords: laboratory tests, fish, catches, pathogens, pollutants.
   
References:

1. Results of monitoring the safety of fish and fish products in the Russian Federation / I. F. Adiatulin, V. I. Belousov, . . Romanenko, A. I. Grudev // Russian journal Problems of veterinary sanitation, hygiene and ecology. 2022. No 3 (43). . 3121319.

2. Byakishev, K. A. Marine fishing law. M., 2007. 560 .

3. Korneiko, O. V. Food security of Russia in the context of fisheries activities / O. V. Korneiko,
. . Dubovik // National security. 2017. No 6. . 2133.

4. Technical Regulations of the Eurasian Economic Union on the Safety of Fish and Fish Products (EAEU TR 040/2016).

5. Khotuntsov Yu.A. Ecology and environmental safety: Textbook. Moscow: Academia, 2002 478 .

  Radiobiology
   
Title:

Change of the mycelial strain Fusarium sambucinum var sambucinum after exposure to ultraviolet irradiation

   
Authors: Dmitry I. Milovankin, Ramzia M. Potekhina
   
Address:

Federal State Budgetary Scientific Institution Federal Center for toxicological, radiation, and biological safety, laboratory of veterinary sanitation, Kazan, Russia

   
Abstract: . The aim of the research was to analyze the survival of the micellar structure of the fungus of the genus Fusarium and morphological changes within 96 hours by means of ultraviolet exposure to the strain Fusarium sambucinum var sambucinum VKPM F-139. The obtained results of the study showed that with a three-time passage of the culture on the nutrient media of Czapek and potato-glucose agar, the strain of the fungus Fusarium sambucinum is restored within 8 days during temperature control at a temperature of 28 ˚C. With repeated passages, an improvement in the growth qualities of the filamentous fungus Fusarium sambucinum was noted, pigmentation and complete recovery occurred at the third passage. On the starved Czapek medium, the time of culture recovery, the formation of spores and conidia, the formation of arachnoid mycelium, and the pigmentation of the fungus were slower than on potato-glucose agar.
   
Keywords: ultraviolet radiation, Fusarium, growth tubes, chlamydospores
   
References:

1. The effect of ultraviolet radiation of a pulsed ARF laser on the viability of microscopic fungi /
I. Y. Kirtsideli, V. A. Parfenov, G. N. Zvereva [et al.] // Optical Journal. 2017. Vol. 84. P. 19-25.

2. Zvereva, G. N. Inactivation of microorganisms under by the action of vacuum ultraviolet radiation / G. N. Zvereva, I. Y. Kirtsideli // Optical Journal. 2021. Vol. 88. P. 67-74.
DOI: 10.17586/1023-5086-2021-88-08-67-74.

3. Potekhina, R. M. Investigation of the field isolate fusarium sporotrichioidesrm+ / R. M. Potekhina // The Veterinarian. 2020. Vol. 4. C. 31-37. DOI: 10.33632/1998-698X.2020-4-31-37.

4. Sazonova, K. V. Influence of ultraviolet irradiation on microscopic fungi isolated from Antarctic habitats / K. V. Sazonova, I. Y. Kirtsideli // Mycology and phytopathology. 2014. Vol. 48. P. 315-321.

5. Evaluation of the viability of fungi after irradiation with longwave ultraviolet light in combination with the introduction of riboflavin. / R. T. Kashiwabuchi, F. R. Carvalho, Yu. A. Khan [et al.] // Graefes Arch Clin Exp Ophthalmol. 2013. Vol. 251. P. 7-15. DOI: 10.1007/s00417-012-2209-z.

6. Brush, J. The effect of repeated low-dose ultraviolet irradiation on the growth of Candida albicans hyphae. / J. Brush, K. Kay. // Mycoses. 2006 Vol. 49 P. 1-5. DOI: 10.1111/j.1439-0507.2005.01169.x.

7. Gagkaeva, T. Fusarium late blight in the Russian Far East: 140 years after the description of the problem of "drunk bread" / T. Gagkaeva, A. Orina, O. Gavrilova // PeerJ. 2021. Vol. 9. P. 46-49. DOI: 10.7717/peerj.12346.

8. Isolation and characterization of the UV mutant Bacillus velezensis with high iturin yield with improved antifungal activity. / Y. T. Kim, S. E. Kim, W. J. Lee [et al.] // PLoS One. 2020. Vol. 15. P. 12-17. DOI: 10.1371/journal.pone.0234177.

9. Nagy, P. The effect of ultraviolet and visible light irradiation on the development of microsclerosis of the fungus Macrophomina Phaseolina. / P. Nagy, G. Fishl // Grain research communications 2002. Vol. 30 P. 383-389.

10. Zvereva, G. N. Features of the impact of UV radiation on microfungi from polar regions /
G. N. Zvereva, I. Ya. Kirtsideli // Processes and phenomena at the border of biogenic and abiogenic nature: A collection of reports presented at the VI International Symposium. Part of lecture notes in the book series Earth System Sciences (LNESS), St. Petersburg, June 24-27, 2018 / St. Petersburg State University. St. Petersburg : Springer Nature, 2020. P. 605-617. DOI: 10.1007/978-3-030-21614-6_32.

  Biotechnology
   
Title:

Dynamics of morphological and bichemical parameters of the blood of first-calf cows when used in the diet of the feed additive BioPrimum dry"

   
Authors: Vadim A. Ruins, Anna A. Kistina, Yuri N. Prytkov, Anna S. Panfilova
   
Address:

N. P. Ogarev National Research Mordovian State University, Agrarian Institute, Saransk, Russia,

kafedra_zoo@agro.mrsu.ru, prutkov@agro.mrsu.ru, annapanfilova568@gmail.com

   
Abstract: The article presents digital material on the effect of the new feed additive BioPrimum dry in the diets of first-calf cows on the biochemical status of blood. It was found that the use of a feed additive in the diet of first-calf cows at a dosage of 75 mg/ kg of dry matter of feed during the experiment, there was an increase in the blood of erythrocytes and hemoglobin by 11,40 and 6,05 %, respectively, compared with analogues of the control group at the beginning of the experiment, and also led to an increase in the amount of total protein in their blood compared to with analogues from the control group by 5,87 % and 4,25 % more than the 1st experimental group (P<0,001), the content of albumins by 9,87 % and globulins by 2,91 % more, compared with the peers of the control group and, respectively, by 6,48 and 2,65 %, than in animals of the 1st experimental group. Positive changes in the blood of experimental animals were also manifested at the end of lactation, a slight increase in the content of erythrocytes and hemoglobin the blood of cows of the 2nd experimental group, stabilization of protein metabolism was revealed. The content of calcium and phosphorus at the beginning of lactation in cows, respectively, of the 1st experimental group by 4,2 % and 4,9 %, in the 2nd experimental group by 22,4 % and 23,2 %, in the 3rd experimental group by 14,0 % and 14,8 % compared with analogues of the control group. A similar pattern in the biochemical parameters of the blood of experimental cows is observed at the end of lactation.
   
Keywords: first-calf cows, diet, feed additive "Bioprimum dry", dose, group, gain, live weight
   
References:

1. Norms and rations of feeding of farm animals // Reference manual 3rd edition revised and supplemented / A.P.Kalashnikov, V.I.Fisinin, V.V.Shcheglov. - Moscow. 2003.- 456 c.

2. Kondrakhin I.P. Methods of veterinary clinical laboratory diagnostics. M.: Kolos, 2004. 520 c.

3. Burmistrov E.N. Clinical laboratory diagnostics: basic studies and indicators.

Moscow, 2002. 19 c.

4. Prytkov Y.N., Kistina A.A., Dorozhkina E.I. Influence of coniferous-energy feed additive on dairy productivity of cows. Agrarian Scientific Journal, 2020, . 5. c.61-63
   
   
Title:

Innovative synbiotics for farm animals and poultry

   
Authors: Larisa A. Neminashcha, Igor V. Pavlenko, Alena A. Kazaku, Tatyana A. Skotnikova, Yuri D. Frolov
   
Address:

All-Russian Research and Technological Institute of Biological Industry (VNITIBP), Department of Quality Assurance of Medicines for Veterinary Medicine and Animal Husbandry, 141142, Russia, Moscow Region, Losino-Petrovsky village, Biokombinat, p. 17.

   
Abstract: