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¹ 5, 2020
Ñêà÷àòü íîìåð æóðíàëà â ôîðìàòå .pdf (2,29 ÌÁ)Toxicology | |
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Title: | CLINICAL, HEMATOLOGICAL AND BIOCHEMICAL PARAMETERS OF BLOOD BY POISONING OF CYANOPIRETHROIDS AND TREATMENT |
Authors: | 1Malanev A.V. - Candidate of Biological Sciences; 1Khalikova K.F. - Candidate of Veterinary Sciences; 1Yamalova G.R., 2Malaneva A.G. - Candidate of Biological Sciences; 1Aleyev D.V. - Candidate of Biological Sciences; 1Egorov V.I. - Candidate of Biological Sciences |
Address: | 1FSBSI «Federal Ñenter for Toxicological, Radiation and Biological Safety» (420075, Kazan, Nauchny gorodok-2, e-mail: vnivi@mail.ru) 2FSBEI HE «Kazan (Volga Region) Federal University» (420008, Kazan, 18 Kremlevskaya
St., e-mail: public.mail@kpfu.ru) |
Abstract: | Due to the increase in the
range and scale of use of pesticides in agricultural practice, as well as
environmental pollution from industrial and household waste, the urgency of the
problem of pesticide poisoning is increasing every year. Because of the
widespread use of pesticides, pests of agricultural crops resistance to them is
gradually increasing, and as a result, there is a growing trend in the use of
mixed pesticides. Cypermethrin causes neurotoxicity by passing through the
blood-brain barrier in the central nervous system, and causes motor deficits.
Also pesticide affects the peripheral nervous system. One of the most important
parts in the complex treatment of cypermethrin poisoning is antidote therapy.
Due to the small amount of information about the treatment of cypermethrin
poisoning in an average fatal dose, the purpose of our research was to study
the effect of cyanopyrethroid (β-cypermethrin) on the hematological and
biochemical parameters of the animals (white rats) blood against of the
therapeutic agents use. The experiments were performed in the vivarium of the
laboratory of medicinal products pharmacology of The Federal Center for Toxicological,
Radiation and Biological Safety. Experiments were conducted on 24 white rats of
180-210 g, β-cypermethrin (Kinmix, LLC "Green Pharmacy of Gardener")
was administered intragastrically at an average fatal dose of 59.6 mg/kg,
therapeutic agents was administered intramuscularly in the development of
typical clinical symptoms of poisoning (depression, bronchospasm, convulsions,
tremor). The animals were divided into 4 groups of 6 animals in each, the first
group was a biological control (common water), the second group – control
(Kinmix). Animals of other groups were received β-cypermethrin and medicinal
preparations in the development of clinical symptoms of poisoning. The third
group was received treatment consisting of dipyroxime (28.0 mg / kg), sodium
bromide (28.0 mg/kg), pyridoxine (0.4 ml) and diosmectite (12.0 mg/kg), the
fourth group – phenazepam (5 mg/kg) and diosmectite (12.0 mg/kg). According to
the results of studies, in animals poisoned with β-cypermethrin in an average
fatal dose (59.6 mg/kg) the best effect was obtained in the group with the use
of dipyroxime, sodium bromide, pyridoxine and diosmectite. As a result, the
survival rate of animals was 83.5%, which is confirmed by hematological and
biochemical studies. |
Keywords: | synthetic pyrethroids, cypermethrin, toxicity, laboratory animals, hematological and biochemical parameters. |
References: | 1.
Changes of hematologic and biochemical indicators at
calfs at the combined intake of pesticide, mycotoxin and heavy metal / V.I.
Egorov, L.R. Valiullin, D.D. Khayrullin –Text: direct // Scientific notes of
the Kazan state academy of veterinary medicine of N.E. Bauman. - 2013. - T.
202. - Page 257. 2.
Current problems of veterinary toxicology / M.Ya.
Tremasov, K.H. Papunidi, E.I. Semyonov, E.Yu. Tarasova –Text: direct // Messenger of veterinary science. - 2012.
- No. 4(63). - Page 16-18. 3.
Efficiency of AL-5 antidote at sharp poisonings with
synthetic pyrethroids / R.M. Aslanov, R.D. Gareyev, A.G. Malanyeva –
Text: direct//Veterinarian. - 2010. - No. 6. - Page 12-14. 4.
Hematologic and biochemical indicators of rats at
sharp intoxication cyanopyrethroids / K.F. Halikova, A.V.
Malanyev, G.R. Yamalova, etc. – Text: direct // Scientific notes of
the Kazan state academy of veterinary medicine of N.E. Bauman. - 2019. - T. 239.
- No. 3. - Page 223-226. 5.
Improvement of a technique of determination of level
of an imidakloprid in sterns by method of a highly effective liquid
chromatography / D.D.Khayrullin., G.R. Yamalova, K.F. Halikova, etc. –
Text: direct // Scientific notes of the Kazan state academy of veterinary
medicine of N.E. Bauman. - 2017. - No. 3. - Page 154-156. 6.
Patomorfologichesky researches of bodies of rats at
poisoning tiaklopridy and use of remedies / V.I. Egorov, K.F. Halikova, D.V.
Aleev, G.R. Yamalova, E.G. Gubeeva –Text: direct // Veterinarian. - 2017. - No.
3. - Page 35-39. 7.
Studying of cytotoxic influence of synthetic in vitro
pyrethroids / A.G. Malanyeva, A.V. Malanyev, V.I.
Egorov, etc. – Text: direct // Russian magazine of the Problem of
veterinary sanitation, hygiene and ecology. - 2015. - No. 2 (14). - Page
92-95. 8.
Biomonitoring of the hematological, biochemical and
genotoxic effects of the mixture cypermethrin plus chlorpyrifos applications in
bovines / D.M. Ferré et al. – Text: direct // Science of The Total Environment.
- 2020. - P. 138058. 9.
Cellular Mechanism involved in cypermethrin induced
neurotoxicity / H.F. Ali – Text: direct // Records of Pharmaceutical and
Biomedical Sciences. - 2020. - Ò.4. - ¹. 1. - P.
32-39. 10.
Changes in some
hematological and biochemical indices of rabbits induced by isoflavones and
cypermethrin / M.I. Yousef et al – Text: direct // Toxicology. - 2003. - Ò. 189. - ¹. 3. - P. 223-234. 11.
Cypermethrin-induced reproductive toxicity in
the rat is prevented by resveratrol / P. Sharma, A. U. Huq, R. Singh – Text:
direct // Journal of human reproductive
sciences. - 2014. - Ò. 7. - ¹. 2. - P.
99. 12.
Detection of cypermethrin residues in toxicological
control objects using gas chromatography-mass spectrometry with solid-phase
extraction / I. M. Fitsev, O. V. Shlyamina, A. R. Makaeva, G. R. Nasybullina,
A. M. Saifutdinov – Text: direct // IJMPRED. -
2020. - V. 10 (3). - P. 5563-5570. 13.
Effect of a beta-cypermethrin and emamectin
benzoate pesticide mixture on reproductive toxicity in male mice in a
greenhouse environment / Y. Zhang , C. Kong, H. Chi et al. –
Text: direct // Toxicology
Mechanisms and Methods. - 2020. - ¹. 30. - P. 100-106. 14.
Protective role
of isoflavones against the toxic effect of cypermethrin on semen quality and
testosterone levels of rabbits / M.I. Yousef, F.M. El‐Demerdash,
K.S. Al‐Salhen – Text: direct // Journal of environmental
science and health, part Â. - 2003. - Ò. 38. - ¹. 4. - P. 463-478. 15.
Toxic effect of
alpha cypermethrin, an environmental pollutant, on myocardial tissue in male
wistar rats / L. Ghazouani et al. – Text: direct // Environmental Science and Pollution
Research. - 2020. - Ò. 27. - ¹. 6. - P. 5709-5717. |
Title: | THE MICROMYCETES PROMOTING EMERGENCE OF GNOYNO-NEKROTICHESKIH OF DEFEATS OF HOOVES OF CATTLE |
Authors: | Potekhina R.M. is a Candidate of Biology; Huzin D.A. - doctor of veterinary sciences; Makayev H.N. is a doctor of veterinary sciences, professor; Semyonov E.I. - Candidate of Biology |
Address: | FSBSI "Federal
Center Toxicological, Radiation and
Biological Safety", (420075, Kazan, Nauchny
gorodok-2, e-mail: vnivi@mail.ru) |
Abstract: | It is purulent - necrotic defeats of a disteel part of extremities of
cattle of varying severity arise owing to traumatizing an integument with the
subsequent contamination of an affected area the gnoyerodny bacteria and
micromycetes of various mushrooms aggravating inflammatory process are one of
the reasons of decrease in efficiency and resistance of an organism of the
cattle to infectious diseases. Results of bacteriological and mycologic
researches of materials from affected areas of a disteel part of extremities of
cattle, tests of forages for animals and the soil from the territory of the
livestock farms delivered from agricultural enterprises of the Republic of
Tatarstan, Stavropol Krai and the Nizhny Novgorod Region where registered
30-40% of animals with signs of damage of extremities are presented in
article. Laboratory
researches of material from the affected extremities, tests of forages and the
soil from the surveyed agricultural enterprises, along with the causative agent
of necrobacillosis F.necrophorum allocated streptococci,
staphylococcus and micromycetes of various mushrooms. The pathogenicity
and virulence of the allocated bacteria, toksigenny properties of isolates of
mushrooms and their role in emergence and distribution of damages of
extremities of the cattle are studied. On the basis of the
received results of researches the conclusion is drawn that on a row with
bacteriological researches of material from the affected extremities of cattle,
carrying out systematic monitoring of maintenance of micromycetes in sterns,
the soil will allow to hold in due time and effectively medical and preventive
events at it is purulent - necrotic damages of extremities of various etiology. |
Keywords: | it is purulent - necrotic damages of extremities, epizootic process, the bacteriological and mycologic analysis of biomaterial, bacteria, mushrooms, tests of forages and the soil. |
References: | 1.
Diseases of fingers and hooves
at cows of a solution problem/Huzin, D.A. – The text: direct // Dairy
industry. - 2019. - No. 3. - Page 62-63. 2.
Kriga, N. Opredelitel of
Berdzhi's bacteria. In 2 t. T. 2: The lane with English / N. Kriga, etc.;
under the editorial office J. Houlta – M.: World, 1997. – T. 1 (in 2 t.) –
482 pages – the Text: direct 3.
Paper bag, A.B. Atlas of
opportunistic mushrooms of the sort Aspergillus of causative agents of
bronchopulmonary infections / A.B.
Kulko. - M.: News, 2012.-155 pages. 4.
Litvinov, M.A. Opredelitel of
microscopic soil mushrooms / M.A. Litvinov. - L.: Science, 1967. – 303 pages. 5.
Methodical recommendations
about diagnostics, treatment and prevention of necrobacillosis, manual
dermatitis and diseases of hooves of cattle of a noncontagious etiology. - M.:
To FGBN Rosinformagrotekh, 2017. – 42 with. – Text: direct 6.
Methodical instructions on
allocation and quantitative accounting of microscopic mushrooms in sterns, feed
additives and raw materials for production of forages. Óòâ. 14.07.03
there is M., 2003. – 8 pages – the Text: direct 7.
Mycologic assessment of
forages in the Republic of Tatarstan / R.M. Potekhina, etc. – the Text:
direct//Veterinarian. - 2019. - No. 1. – Page 19-24. 8.
Prevention of diseases of
hooves of KRS of optimization of feeding, leaving and contents / Huzin,
D.A. – Text: direct//Veterinary science and feeding. - 2013. - No. 3. –
Page 24-25. 9.
Etiology, pathogenesis and
measures of fight against necrobacillosis of cattle / H.N. Makayev,
etc. – the Text: direct // Scientific notes of the Kazan state
academy of veterinary medicine of N.E. Bauman. - 2012. - T.209. – Page 202-206. |
Title: | DETERMINATION OF ACUTE AND CHRONIC TOXICITY OF THE ANTIDOTE RR-2 ON HYDROBIONTS |
Authors: | Aslanov R.M. – Doctor of Biological Sciences, Professor, Makaeva A.R. – Candidate of Biological Sciences, Makaeva V.I., Almitova L.I. |
Address: | FSBSI «Federal Center for Toxicological, Radiation and Biological Safety» (420075, Kazan, Nauchny gorodok-2, e-mail: vnivi@mail.ru) |
Abstract: | Organophosphate
pesticides occupy one of the leading places among the preparations used in
agriculture. Their high efficiency in the protection of animals and plants has
been proved. Organophosphate pesticides are successfully used in the
agricultural industry as insecticides and acaricides. At the same time, despite
the proven feasibility of use in the national economy, pesticides are
characterized by a high danger to humans and animals. The current situation
with the treatment of poisoning with organophosphate pesticides necessitates
the development of new domestic effective antidotes, one of the main components
of this ones are holinolytics. The article presents the results of experimental
studies aimed at studying the parameters of acute and chronic toxicity of the
new domestic antidote RR-2 synthesized in the FCTRBS. Experiments were carried
out on pond fish and guppies. For this purpose, the animals were kept in
antidote solutions. Survival, behavior, and weight of specimens were evaluated.
According to the results of experiments, the acute toxicity of cholinolytic for
ponds is 150.00±13.8 mg/dm3, for fish – 125.99±12.82 mg/dm3. |
Keywords: | antidote, organophosphate pesticides, toxicity, hydrobionts, poisoning. |
References: | 1.
New antidotes at poisoning of animals with
fosfororganichesky and carbaobscene pesticides / R.M. Aslanov, I.G.
Hayrutdinov, N.N. Zhestkov, etc. – the Text: direct // Veterinarian. –
2005. – No. 2. – Page 7-9. 2.
Application it is punched - the analysis in toxicology
and pharmacology with use of the Microsoft Excel program for assessment of
pharmacological activity at an alternative form of accounting of reactions /
P.N. Babich, A.V. Chubenko, S.N. Lapach – the Text: direct // Modern
problems of toxicology. – 2003. – No. 4. – Page 81-89. 3.
Water fleas as a test object in the
toksikogeneticheskikh researches / T.P. Denisova, E.V. Simonova – the
Text: direct // the 3rd congress of toxicologists of Russia: theses of reports,
on December 2-5, 2008 / under the editorship of G.G. Onishchenko, B.A.
Kurlyandsky. – M, 2008. – Page 93-95. 4.
Genotoxicity mechanisms the fosfororganicheskikh of
connections / E.L. Levitsky, A.N. Marchenko, Yu.I. Gubsky – the Text:
direct // Modern problems of toxicology. — 1998. — No. 1. — Page 47-50. 5.
Assessment of toxicity of copper for hydrobionts /
Makayev, A.R. is the Text: direct//Veterinarian. – 2015. – No. 6. – Page
32-36. 6.
Methodical instructions on development of standards of
water quality of water objects of fishery value, including standards of
threshold limit values of harmful substances in waters of water objects of
fishery value: No. 14702: óòâ. Rosrybolovstvo 04.08.09, 2009 – the Text: direct. 7.
Studying of biological oxidation of in vitro for the
accelerated forecasting of metabolic activity of industrial poisons / A.D.
Frolova, L.V. Lukovnikova – the Text: direct // the Toxicological
messenger, – 1995. – No. 4 – Page 9-12. 8.
Ethics of a medicobiological experiment in preclinical
tests / G.P. Chervonskaya, G.P. Pankratova, L.L. Mironova, etc. – the
Text: direct // Toxicological messenger. 1998. – No. 3. – Page 2-8.
9.
Sharp poisonings with fosfororganichesky connections:
the main clinical syndromes and mechanisms of their formation / O.A. Harchenko,
G.M. Balan, N.N. Bubalo – the Text: direct//Modern problems of toxicology.
– 2013. – No. 1-2. – Page 17-31. |
Title: | ASSESSMENT OF TOTAL TOXICITY OF FEED REPUBLIC OF TATARSTAN |
Authors: | Sagdeeva Z.H., Ermolaeva O.K. - candidate of biological Sciences; Valiev A.R - candidate of biological Sciences; Sagdeev D.R., Potekhina R. M. - candidate of biological Sciences; Matrosova L.E. - doctor of biological Sciences |
Address: | FSBSI «Federal Center for Toxicological, Radiation and Biological Safety» (420075,
Kazan, Nauchny gorodok-2, e-mail: vnivi@mail.ru) |
Abstract: | The main requirements for feeds are their safety and nutrition, ensuring
the physiological needs of animals. Safety indicators include toxicity, the
content of toxic elements, mycotoxins, pesticides, nitrates and nitrites,
conditionally pathogenic and pathogenic microflora. The purpose of the research
is to assess the general (integral) toxicity of feed received from the Republic
of Tatarstan (Zelenodolsky, Nurlatsky, Buinsky, Baltasinsky, Arsky, Laishevsky
districts, Naberezhnye Chelny). The experiments were carried out in the
laboratory of mycotoxins «FTSTRB-VNIVI». In the studied feeds, the general
toxicity was determined according to GOST 31674-2012 for 3 types of test objects
(stylonichia, white mice, rabbits). 89.8% of feed samples by bioassay on
protozoa showed no toxicity; 4.1% were toxic. In all cases, the bioassay on
white mice was negative (no death of the animals and no visible changes in the
internal organs during the autopsy of the animals killed were observed). In two
cases, dermatonecrotic effect was revealed when applying extracts of the
studied feed to the skin of rabbits. It is worth considering the fact that the
official interpretation of the research results requires adjustment depending
on the type of animals. In the case of toxicity on protozoa and the absence of
toxicity on white mice and rabbits, the food is officially considered
non-toxic, but given that the cicatricial microflora, including ciliates, plays
a special role in digestion in ruminants, it must be considered toxic. Most of
the studied samples of feed from livestock farms of the Republic of Tatarstan
by the bioassay method were recognized as benign and recommended for use
without restriction. For livestock farms where toxic feed has been discovered,
recommendations have been issued for their further use. |
Keywords: | agricultural feed, bioassay, toxicity, stilonychia, white mice, rabbits |
References: | 1. Biotesting of products forages and objects of the environment/VA. Debts,
S.A. Lavin, S.S. Kazak, etc. – the Text: direct//RUDN Bulletin. – 2014. - No.
3. – Page 63-72. 2. Influence of a complex of zeolite and the shungit for resistance and
efficiency of broilers at the mixed mikotoksikoz / N.N. Mishina, E.I. Semyonov,
K.H. Papunidi, R.M. Potekhina, S.A. Tanaseva, O.K. Yermolaeva, Z.H. Sagdeeva,
D.H. Gataullin – the Text: direct//Veterinarian. - 2018. - No. 6. - Page
3-9. 3. Studying of hematologic and biochemical indicators of blood of sheep at
introduction of a moniliformin and application of sorbents / O.K. Yermolaeva,
S.A. Tanaseva – the Text: direct//Veterinarna medicine. – 2013. - No. 97.
– Page 324-326. 4. Immunosuppression in pathogenesis of T-2 of a mikotoksikoz and its
pharmakokorrektion / A.R. Valiyev, E.I. Semyonov, F.G. Akhmetov – the
Text: direct//Veterinarian. - 2011. - No. 2. - Page 4-6. 5. Clinical and patomorfologichesky changes at the spontaneous associated
T-2 and an aspergillotoksikoza of hens / N.V. Saprinskaya, I.Yu. Domnitsky,
V.A. Agoltsov, O.M. Popova – the Text: direct//Scientific life. – 2016. -
No. 8. – Page 27-39. 6. Mikotoksikologichesky assessment of forages in the Republic of
Tatarstan / E.I. Semyonov, N.N. Mishina, S.A. Tanaseva, R.M. Potekhina,
Z.H. Sagdeeva – the Text: direct//Works of the Federal Center of health
protection of animals. – 2018. - T.16. – Page 512-523. 7. Mycotoxins - real threat of food security / M.Ya. Tremasov, A.V. Ivanov,
E.Yu. Tarasova – the Text: direct//Messenger of veterinary science. – 2013.
- No. 2. – Page 78-80. 8. Monitoring of aflatoxins in sterns from the Ryazan region / S.A.
Tanaseva, I.V. Bosyakov – the Text: direct//Materials of the international
scientific and practical conference devoted to the 90 anniversary since the
birth of professor V.A. Kirshin "Current problems of veterinary
medicine". – 2018. – Page 191-193. 9. Morphological and biochemical indicators of blood of rabbits at an
experimental aflatoksikoz against the background of use of Retinolum of acetate
and zeolite / A.Z. Mukharlyamova, A.M. Tremasova, S.A. Tanaseva, N.G.
Shangarayev, P.V. Sofronov, E.I. Semyonov – the Text: direct//Scientific
notes of the Kazan state academy of veterinary medicine of N.E. Bauman. - 2019.
- T. 238. - No. 2. - Page 133-138. 10.
About results of the analysis of
forages on the content of mycotoxins/L. G. Burdov, L.E. Matrosova – the
Text: direct//Veterinarian. - 2011. - No. 2. – Page 7-9. 11. The combined effect of dioxine, mycotoxins and toxic elements on animals
/ I.R. Kadikov, V.R. Saitov, K.H. Papunidi, M.Ya. Tremasov, I.I.
Idiyatov – the Text: direct//Veterinary science. 2014. No. 9. Page 47-51. 12.
T-2 toxin biotesting for
Stylonychia mytilis and daphnia magna straus / E.A. Shuralev, L.R. Valiullin,
O.V. Nikitin, E.I. Semyonov – the Text: direct//Progress of a medical
mycology. – T.17. - 2017. - Page 457-452. 13.
Toxicological assessment of
forages from the Republic of Mordovia on availability of pesticides and
nitrogen-containing connections / A.V. Malanyev, D.V. Aleev, G.G.
Galyautdinova, V.I. Egorov, etc. – the Text: direct//Veterinarian. – 2019. -
No. 2. – Page 43-49. 14.
Toxicological assessment of the
combined influence of a detsis, T-2 of toxin and cadmium on an organism of
calfs at the level of maximum allowable concentrations / of Galyautdinov, V.I.
Egorov – the Text: direct//Veterinary medicine. – 2013. - No. 97. – Page
418-419. 15.
The toxicological analysis of some
forages on reaction of survival of an infusorian of Paramecium caudatum / I.A.
Shadrin – the Text: direct//KRASGAU Bulletin. - 2008. - No. 2. - Page 128-134. 16.
Ecotoxicological monitoring of
agricultural and food products in the Republic of Tatarstan/VA. Konyukhov, I.R.
Kadikov, A.A. Korchemkin, I.F. Vafin – the Text: direct//Materials of the
international scientific and practical conference devoted to the 90 anniversary
since the birth of professor V.A. Kirshin "Current problems of veterinary
medicine". – 2018. – Page 145-148. 17.
Joint effect of the
mycotoxins T-2 toxin, deoxynivalenol and zearalenone on the weaner pigs against
a background of the infection load / E.I. Semenov, L.E. Matrosova, M.Ya.
Tremasov, E.Yu. Tarasova, M.A., Kryuchkova et al. – Text: direct // Research journal of pharmaceutical, biological and
chemical sciences. – 2016. - 7(1). – Ð.1860–1868. |
Radiobiology | |
---|---|
Title: | USING AN ENZYME IMMUNOASSAY TO DETERMINE HUMORAL FACTORS OF NATURAL RESISTANCE IN IRRADIATED RATS |
Authors: | Kobyalko V.O. - Candidate of Biology, Sarukhanov V.Ya. – Candidate of Biology, Polyakova I.V., Gubina O.A. |
Address: | FSBSI "Russian Research Institute of Radiology and
Agroecology", (249032, Kaluga region,
Obninsk, Kiyevskoye Highway, 109 km, e-mail:
nar@obninsk.org, sarukhanov.vladimir@yandex.ru) |
Abstract: | Determining the level of
natural resistance is essential for assessing the state of the human body and
animals, the severity of the development of negative processes and the forecast
of consequences when exposed to man-made environmental factors. The available
methods for determining humoral factors of natural resistance are
labor-intensive and require large amounts of test serum or plasma, reagents,
and test culture. This makes it difficult to use them for a large number of
samples in model Toxicological and radiobiological studies, as well as for mass
inspection of farm animals in technogenically polluted territories. Automation
of the detection process with the help of technical solutions used for enzyme
immunoassay can help solve this problem successfully. Studies have shown that
the values of lysozyme, β-lytic and bactericidal activity of the blood of
irradiated rats, determined using standard methods, and ELISA do not differ
significantly. The dynamics of the studied humoral factors of natural
resistance in animals after radiation exposure in the dose range from 3 to 12
Gy depended on the radiation dose, and not on the method of determination. It
was demonstrated that the use of ELISA to determine the level of natural
resistance of animals allows to: decrease the volume of the studied serum or
blood plasma test and culture; to increase the number of simultaneously
analyzed samples, to measure parallel samples to maximize the accuracy of the
studied parameters. |
Keywords: | natural resistance, laboratory animals, blood, lysozyme, β-lithionic, activity, detection methods, enzyme immunoassay |
References: | 1.
Skopichev, V.G. Animal physiology
and ethology / V.G. Skopichev. T.A. Eysymont, N.P. Alekseev, I.O. Bogolyubova,
A.I. Enukashvili, L.Yu. Karpenko – M.: Ear, 2013. - 720 pages – the Text: direct.
2.
Skopichev, V.G. Animal physiology
and V.'s ethology of / Skopichev. T.A. Eysymont, N.P. Alekseev, I.O.
Bogolyubova, A.I. Enukashvili, L.Yu. Karpenko – M.: An ear, 2013.-720 pages – the Text:
direct.
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Bukharin, O.V. Sistema of a beta
lysine and her role in clinical and experimental medicine / O.V. Bukharin, N.V.
Vasilyev - Tomsk, 1977. - 187 pages – the Text: direct.
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Bukharin, O.V. Lizotsim and his
role in biology and medicine / O.V. Bukharin, N.V. Vasilyev - Tomsk, 1974. -
209 pages – the
Text: direct.
5.
Symptomatology of radiation
sickness and natural resistance of calfs after total radiation / V.Ya.
Sarukhanov, V.O. Kobyalko – the Text: direct//Veterinarian. – 2019. - No. 4. -
Page 36-40.
6.
Features of a course of sharp radiation sickness of
sheep against the background of respiratory diseases / V.Ya. Sarukhanov,
N.N. Isamov, L.A. Bastrakova, V.O. Kobyalko – the Text: direct//Radiation and
risk. - 2017. - T. 26 - No. 4. - Page 124-132. 7.
Method of determination of beta and lytic activity of
blood of farm animals
/ V.Ya. Sarukhanov,
N.N. Isamov, P.G. Tsarin – the Text: direct//Agricultural biology. - T. 42. -
No. 4 - 2007. - Page 123-125. 8.
Method of determination of activity of lysozyme of
blood of farm animals /V.Ya. Sarukhanov, N.N. Isamov, I.M. Kolganov – the Text:
direct//Agricultural biology. - 2012. - No. 2. - With 119-123. 9.
Modification of a method of determination of
bactericidal activity of blood of farm animals / V.Ya. Sarukhanov, N.N. Isamov,
E.B. Mirzoyev, V.O. Kobyalko – the Text: direct//Agricultural biology - 2007. -
No. 2. – Page 119-122. 10.
Assessment of
consequences of external γ-radiation and the
early forecast of an outcome of sharp radiation sickness of sheep / V.Ya.
Sarukhanov, N.N. Isamov, V.G. Epimakhov – the Text: direct//Veterinarian. -
2015. - No. 1. - Page 29-33 11.
Beta and lytic
properties of blood, at action of ionizing radiation / V.Ya. Sarukhanov, N.N.
Isamov – the Text: direct//Agricultural biology – 2005 - No. 2 - Page 116-118. 12.
Influence of bacterial medicines on survival
of the irradiated animals / V.N. Maltsev, K.K. Gutsenko, N.V.
Emchenko, A.G. Shvachko – the Text: direct//Radiation
biology. Radio ecology. - 1994. - T. 34. - Issue 4-5. - Page 578-581. 13.
European
convention for the protection of vertebrate animals used for experimental and
other scientific purpose. Cet ¹:123. Date 18.03.1986. Status as of:15.03.2010 |
Title: | RADIATION TECHNOLOGIES AS A WAY TO ENSURE MICROBIOLOGICAL SAFETY OF FOOD PRODUCTS FOR CONSUMERS WITH IMMUNE DEFICIENCIES |
Authors: | Sarukhanov V.Ya. - Candidate of Biology, Kobyalko V.O. - Candidate of Biology, Polyakova I.V. |
Address: | FSBSI "Russian Research Institute of Radiology and Agroecology" (249032, Kaluga region, Obninsk, Kiyevskoye Highway, 109 km. e-mail: nar@obninsk.org) |
Abstract: | Food products, ready-to-eat and semi-finished
products of animal origin, due to the peculiarities of manufacture and storage,
can be a source of intestinal infections and such dangerous diseases as
listeriosis and erisipiloid (erysipelas). The most at risk are consumers with
immunodeficiency during the rehabilitation period, after the use of
immunosuppressants, cytotoxic drugs and ionizing radiation during the treatment
of cancer. If infection with banal intestinal infections and listeriosis occurs
alimentary, then an erisipeloid can develop after contact with the causative
agent of the disease during cutting and preparation of products from pork
obtained from animal carriers of bacteria. A study of the possibility of using
radiation technologies to ensure microbiological safety of problematic food
products showed that irradiation of minced meat in a vacuum package at a dose
of 1.5 kGy leads to complete inactivation of the listeriosis pathogen. A
positive effect was observed during the radiation processing of minced meat
(dose of 0.5 kGy) in a modified gas environment (75% O2, 25% CO2).
It was shown that the magnitude of the effective dose depends on the
concentration of Listeria monocytogenes in production. The fact that the
pathogen of erisipeloid does not form spores and capsules (radioresistant
forms) suggests that the inactivating dose will not exceed 3 kGy. According to
numerous data, radiation treatment in this dose range does not reduce the
quality of food products. Thus, radiation technologies can be considered as the
best way to provide people with immunodeficiencies with safe and quality food
products. |
Keywords: | radiation technologies, listeriosis pathogens, erysipeloid, immunodeficiency, radiation treatment, food products, semi-finished products, minced meat, quality indicators |
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VETERINARY VIROLOGY, EPIZOOTOLOGY AND ANIMAL THERAPY | |
---|---|
Title: | DYNAMICS OF THE FUNCTIONAL ACTIVITY OF THE PHAGOCYTIC CELLS OF ANIMALS VACCINATED AGAINST ANTHRAX. |
Authors: | Ivanova S. V. - Candidate of Biological Sciences, Melnikova L. A. – Candidate of Veterinary Sciences, Rodionov A. P |
Address: | FSBSI "Federal Center for Toxicological, Radiation and Biological Safety" (420075, Kazan, Nauchny gorodok – 2, e-mail:
vnivi@mail.ru) |
Abstract: | The leading role in elimination of infectious agents
from the body, such as anthrax, belongs to cells with phagocytic properties,
immunoglobulins and proteins of the complement system. The paper presents the
materials of studying the dynamics of the functional activity of phagocytes of
animals vaccinated against anthrax. Functional activity of phagocytic cells was
evaluated by the phagocytic activity of leukocytes. Phagocytic activity (FA)
was determined in calculating the relationship of phagocytic neutrophils to the
total number identified and their absorption capacity for the phagocytic index
(PHI) – the number of microbial cells in terms of one active neutrophil. During
the study observed the increase in the values of phagocytic activity and
phagocytic index, which reached their maximum values at day 14 after
immunization, indicating activation of factors of natural resistance of the
organism. High values of the studied parameters was observed up to 150 days
after vaccination. In the further periods of the study, indicators fell to
levels identical to the control group. The decrease in the examined values to
the level of the control group determines the low efficiency of presentation of
antigens to cells of the adaptive immune system, and as a result, the actual
phagocytosis – final stage of the immune response. Reducing the number of
active phagocytes to 120 days after vaccination may be associated with complete
elimination of the introduced vaccine strain from the body by this time. |
Keywords: | anthrax, immunity, phagocyte, phagocytic number, phagocytic index |
References: | 1. Allergology and
immunology. National guide / R. M. Khaitov. – M.: GEOTAR-Media. - 2009. – 656
p. – Text: direct. 2. Gilmutdinov, R. Ya.
infectious diseases of exotic and wild animals / R. Ya. Gilmutdinov, A.V.
Ivanov, A. N. Panin. - M.: Kolos, 2010. - 668s. – Text: direct. 3.
Using
geoinformation systems to create an electronic database of anthrax burials on
the territory of the Stavropol territory / N. P. Buravtseva, V. M. Mezentsev,
A. G. Ryazanova, T. M. Golovinskaya, D. Yu.Degtyarev, A. N. Pazenko, O. I.
Semenova, A. N. Kulichenko – Text: direct //Problems oobo dangerous
infections.- 2019. - No. 4.-P. 31-36. 4. Kulichenko, A. N.
anthrax in the North Caucasus / A. N. Kulichenko, N. P. Buravtseva, A. G.
Ryazanova, E. I. Eremenko; edited by prof. – Maykop: Quality. - 2016. – 198 p.
– Text: direct. 5. Simonova, E. G.
epidemiological danger of anthrax burials: theoretical and methodological
aspects / E. G. Simonova, S. A. Kartavaya, M. N. Loktionova, V. I. Ladny –
Text: direct // Medicine in Kuzbass. – 2013. - no. 2. – Pp. 26-31. 6.
Improving
methodological approaches to the survey of anthrax graves and animal burial
grounds / Z. F. Dugarzhapova, M. V. Chesnokova, T. A. Ivanova, S. A. Kosilko,
S. V. Balakhonov – Text: direct // Problems of particularly dangerous
infections.- 2019. - No. 4.- P.
41-47. 7. Old anthrax burials -
possible sources of infection spread / A. K. Galiullin, S. A. Klimina – Text:
direct // Veterinary doctor.-2004.-¹3-4.-p. 36-38 8. Characteristics of the
epizootic situation for anthrax in the Republic of Tatarstan / A. P. Rodionov,
S. V. Ivanova, L. A. Melnikova, Kh. N. Makaev, A. G. Khisamutdinov, A. S.
Kozlov – Text: direct // Veterinary science. - 2020. - No. 3. – P. 8-11. |
Title: | OBTAINING RABIES VIRUS ANTIGEN BY THREE-PHASE EXTRACTION |
Authors: | Akhmadeev R. M. – Ñàndidate of Veterinary Sciences, Mukhamedzhanova A.G., Miftakhov N. R. – Ñàndidate of Biological Sciences; Nasyrov Sh. M. – Ñàndidate of Veterinary Sciences; Samerkhanov I. I. – Ñàndidate of Biological Sciences; Aleeva Z. Z. , Arutunjan G. S., Yarullina G. M. |
Address: | FSBSI «Federal Center for Toxicological, Radiation and Biological Safety» (420075,
Kazan, Nauchny gorodok-2, e-mail: vnivi@mail.ru) |
Abstract: | Results of research of an alternative way of release of antigen of a virus of the rage based on a method of three-phase extraction of initial suspension and scoring on a dispergator and also estimates of immunochemical properties of the received product are presented. Initial virussoderzhashchy material of organ and fabric origin was subjected to extraction by serial processing by saturated solution of sulfate of ammonium (NSA) and a butanol. For fuller disintegration the extracted suspension was subjected to scoring on an ultrasonic dispergator of UZDN - 2T then anti-gene samples processed 96% ethanol in different proportions. Anti-gene properties of the received fractions estimated by methods of an analytical disk-electrophoresis, a western - a blotting and "sandwich" - IFA. It is shown that application of ultrasonic processing of the extracted suspension of virussoderzhashchy material and further processing of 96% ethanol in the ratio 1:1 allows to get rid of ballast substances and subvirus components more effectively. The received anti-gene product is monomeric, supports one prevailing proteinaceous fraction weighing 67.2 kd that corresponds to G-protein of a virus of rage and also has a caption in "sandwich" - IFA 1:40960. These characteristics confirm high diagnostic efficiency of this antigen and its suitability for a complete set of express test systems on the basis of IFA. The described way of release of antigen by means of chemicals and ultrasonic processing can be considered as an available and economic alternative to the considered earlier zone ultracentrifugation in density gradients. |
Keywords: | rage virus glycoprotein, immunofermental analysis, designing of diagnostikum. |
References: | 1.
Results of test "A set of
medicines for diagnosis of clamidiosis of pigs by method of the immunofermental
analysis / V.V. Evstifeev – the Text: direct//Scientific notes of the Kazan
state veterinary academy of N.E. Bauman. – 2015. – No. 1. – Page 71-73. 2.
Allocation, cleaning and
assessment of serological activity of anti-gene components of a virus of rage /
M.A. Yefimova, K.S. Hayertynov, A.F. Arslanova, etc. – the Text:
direct//Problems of especially dangerous infections. – 2017. – No. 4. – Page
27-31. 3.
Assessment of suitability of
the globulins emitted the antirabicheskikh for methods of laboratory
diagnostics: IFA and MFA / M.A. Yefimova, A.G. Mukhamedzhanova, A.N. Chernov,
etc. – the Text: direct//Veterinarian. – 2018. – No. 4. – Page 3-7. 4.
Modern problems of vaccinal
prevention of rage / A.A. Movsesyants, Yu.V. Olefir – the Text:
direct//Biological products. Prevention, diagnostics, treatment. – 2019. – No.
19 (1). – Page 10 -16. 5.
Mukhamedzhanova, A. G.
Improvement of ways of receiving high cleaning antigen of a virus of rage for
express test systems on the basis of IFA and MFA: the abstract of the thesis
for a degree of the candidate of veterinary sciences / Mukhamedzhanova Antonina
Glebovna – Kazan, 2020. – 24 pages. 6.
Rakhmanin, P. V.
An immunofermental test system for determination of level the antirabicheskikh
of antibodies in serums of blood of the cats imparted against rage and dogs:
the abstract of the thesis for a degree of the candidate of veterinary sciences
/ Rakhmanin Pyotr Vladimirovich – Shchyolkovo,
2009. – 29 pages. 7.
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Federation Virus-like particles (VLP) from a rage virus glycoprotein: îïóáë.
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purification. – 2020. – 168:105567. |
Title: | SOME PRODUCTIVE AND BIOLOGICAL FEATURES OF BULL-CALVES OF VARIOUS GENOTYPES AT CULTIVATION ON THE FEEDLOT |
Authors: | 1Azhmuldinov E.A. – doctor of agricultural sciences, professor; 1Kovalenko V. P. Accusative – the candidate of agricultural sciences; 1Titov M.G. - the candidate of agricultural sciences; 1Kizayev M.A. – candidate of agricultural sciences; 2Rogozinnikova I.V. – Candidate of Biology. |
Address: | 1FSBSI "Federal Scientific Center of Biological
Systems and Agrotechnologies of the Russian Academy of Sciences" (460000, Orenburg, January 9 St., 29, tel/fax (3532) 30-81-70) 2FSBEI HE “Ural State Agricultural University (620075, Yekaterinburg, Karl Liebknecht St., 42) |
Abstract: | The experiment provides information
on a comparative study of the biological characteristics and productive traits
in bull-calves of various genotypes under the year-round feedlot conditions, OOO,
conducted a scientific and economic experiment. The scientific and economic
experiment was conducted on three groups (30 animals each) of bull-calves of
Kazakh white-headed (I group), Simmental (II group) and Kalmyk (III group)
breeds in Yubileynoye LLC, Adamovsky District, Orenburg Region to achieve
appointed goals. The test animals were kept on a feedlot combined with a light
type building for resting and feeding individuals in bad weather. All
technological processes associated with keeping and feeding animals were
mechanized. During the experiment, bulls of all experimental groups received
the same rations, consisting of mixed grass hay, haylage, corn silage,
concentrates in accordance with detailed feeding norms, taking into account the
receipt of 800 - 900 g of daily gain in body weight. The diets were balanced by
basic nutrients and adjusted according to live weight and growth rate of
individuals of various genotypes. During the experiment, the average monthly
outdoor temperature at the feedlot in autumn was from + 10.0 to -5.5 ° C, in
winter from -10.8 to -15.7 ° C, in spring from -8.8 to + 9,9 ° C. Relative
humidity ranged from 55 to 86%, wind speed from 0.3 to 9.7 m / s. As a result
of the study, it was found that Simmental bull-calves had the highest live
weight and absolute growth rate. They were ahead of their beef cattle peers in
the first indicator by 12.5 (3.06%) and 28.0 kg (7.13%), in the second - by 6,4
(3.6%) and 13.3 kg (7.9%). In general, the average daily gain over the period
of the experiment of bull-calves from groups I, II, and III was 836; 866 and
803 g with maximum performance in individuals of the Simmental breed. However,
the bull-calves of the Kazakh white-headed and Kalmyk breeds were more
resistant to the influence of environmental factors. They exceeded the
comparable peers of the Simmental breed in terms of bactericidal activity of
blood serum by 5.2 and 4.0%, serum lysozyme activity - by 1.4 and 1.6 times in
unfavorable months by climatic conditions. The greatest activity of β-lysines
was observed in individuals of all groups in the winter (15.2 - 17.6%), lower -
in fall (14.9 - 15.7%). |
Keywords: | bulls-calves, genotype, live weight, average daily gain, nonspecific resistance |
References: | 1. 1. Baktygaliyeva A.T. Productive qualities and biological features of young growth of the Kazakh white-headed breed of different genotypes: the abstract of the thesis for a degree of candidate of science / Baktygaliyev, Asemgul Temirkhanovna, Orenburg, - 2017. - 25 pages – the Text: direct 2.
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Influence of technology of contents on natural
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breeding. - 2003. – Issue 56. – Page 284-288. 4.
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fundamentals of technology of meat cattle breeding / Cherekayev A.V. – the
Text: direct//Zootechnics. - 1995.-No. 6. - Page 16-20. |
Title: | IMPROVING THE QUALITY OF FOOD EGGS BY USING WATER-SOLUBLE VITAMINS IN THE FEEDING OF LAYING HENS |
Authors: | Alekseev V.A. - Doctor of Agricultural Sciences, Nemtseva E. Yu. - Candidate of Agricultural Sciences, Terent'ev A. Yu. - Candidate of Agricultural Sciences, Andreeva S. G. - Candidate of Agricultural Sciences |
Address: | FSBE HE «Chuvash State Agricultural Academy», (428003,
Cheboksary, Karl Marx Str., 29, å-mail:
EUNemtzeva@yandex.ru) |
Abstract: | In two
scientific and economic experiments conducted by the group-analog method in the
conditions of a poultry farm in the Chuvash Republic, the effect of feeding
preparations of water-soluble vitamins C, Bc and B13 to laying hens of the
cross "Loman-brown" on their egg production and egg quality was
studied. In the first experiment, the birds of the experimental groups were
given 50 mg of ascorbic acid, 1 mg of folic acid, and 5 mg of potassium orotate
for 180-230 days as part of 1 kg of compound feed PK-1-3. In the second
experiment, we studied the effectiveness of introducing potassium orotate into
compound feeds for laying hens at doses of 5.0, 7.5 and 10.0 mg per 1 kg of
feed. Such feeding contributed to an increase in egg-laying hens of the
experimental groups in comparison with the control groups by only 3.0 – 5.8 %,
with a significant improvement in the vitamin value of food eggs. In the first
experiment, there was an increased content of thiamine, Riboflavin, ascorbic
acid and retinol in the yolk of eggs by 20% compared to the control group. When
studying the effectiveness of introducing into feed of vitamin B13, it is
established that at the dose of 5 – 7.5 mg/kg was an increase in egg yolk of
laying hens of the experimental groups compared with the control carotenoids
4.5 to 7.1 %, vitamin A – 52 – 59 %, thiamin – 14.3 – 28.6 per cent. In 1 kg of
feed, the content of ascorbic acid should be at least 75 mg, folic acid-102 mg,
potassium orotate-7.5 mg. |
Keywords: | laying hens, poultry, feed, feeding, vitamins, eggs, quality. |
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