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  Editorial
   
Title:

FORMATION OF THE DIGESTIVE-TRANSPORT CONVEYOR OF CARBOHYDRATES IN POSTNATALLY PHYSIOLOGICALLY IMMATURE ANIMALS

   
Authors: N.Sh. Khasnutdinov - Candidate of Biological Sciences, Associate Professor
   
Address:

FSBEI HE Volga State Academy of Physical Culture, Sports and Tourism,

(420010, Republic of Tatarstan, Kazan, Universiade Village, 35, e-mail: nahas@mail.ru)

   
Abstract:

. 170 180 . . 21 . , 8 . . - . 10 , . - (, , ). 3- . , , 1 ( 3,7) 200 % , , 30-60 60 .

   
Keywords: mucosal-serous transport, incubation of enzymatically active and transport preparations.
   
References:

References

1. Ugolev, A.M., The study of the digestive apparatus in humans. / A.M. Ugolev, N.N. Jesuit, and others. C.G. Mosevich  L.: Science. 1969.  215 .

2. Ugolev A.M. Membrane digestion. Polysubstrate processes, organization and regulation. /            A.M Ugolev L .: Science.  1972. 358.

3. Ugolev A. M. The evolution of digestion and the principles of evolution of functions. / A.M. Ugolev L .: Science. 1985.  543 .

4. Rakhimov K.R., Demidova A.I. Carbohydrates and mechanisms of their assimilation. /                           K.R. Rakhimov, A.I. Demidova  Tashkent: Fan.  1986. 132 .

5. Rakhimov K.R. Digestion and absorption of carbohydrates in the small intestine of growing rabbits / K.R. Rakhimov. // Fiziol. journal THE USSR. 1986 - 2.- .35

6. Rakhimov K.R., Demidova A.I. Enzymes of the initial and final stages of the digestion of carbohydrates in the ontogenesis of mammals // Successes sovr. biol. 1987. T. 104. Issue 1 (4). S. 22.

7. Khasnutdinov N.Sh. Postnatal ontogenesis of the digestive transport conveyor of carbohydrates. Abstract. diss. ... cand. biol. Sciences: 03.07.02. / N.Sh. Khasnutdinov. Kazan, 2002. 18 p.

8. Khasnutdinov N.Sh. Formation of the digestive transport conveyor of carbohydrates in postnatally physiologically immature animals // N.Sh. Khasnutdinov. Veterinarian. 2019. No. 3. C 46.

9. Anderson C.M., Messer M., Townley R.R. W. Freeman M., Robinson M.J. / C.M., Anderson,                  M. Messer, R.R.W. Townley, M. Freeman, M.J. Robinson // Intestinal isomaltase deficiency in patients with hereditary sucrose and starch intolerance. Lancet. 1962. N. 7255. P. 556.

10. Wilson T.H., Wiseman G. The use of sacs everted small intestine for the study of the transference of substances from the mucosal to the serosal surface. / T.H. Wilson, G. Wiseman. // J. Physiol. 1954. V. 123. P. 116.

 

 

 

 

  Toxicology
   
Title:

STUDY OF THE FIELD ISOLATE FUSARIUM SPOROTRICHIOIDES RM+

   
Authors: Potekhina R. M. - andidate of Biological Sciences
   
Address:

FSBSI Federal Center for Toxicological, Radiation and Biological Safety,

(420075, Kazan, Nauchny gorodok-2, e-mail: vnivi@mail.ru)

   
Abstract:

Field isolates of fungi of the genus Fusarium are widely distributed in the environment, producing under certain conditions highly toxic metabolites, in particular T-2 toxin. The aim of the research is to study the effect of ultraviolet irradiation on the production of the t-2 toxin Fusarium sporotrichioides. The experiments were carried out in the laboratory of the Federal State Budgetary Institution "FCTRBS-RRVI". The object of the study was a field isolate Fusarium sporotrichioides RM+, isolated from a sample of feed containing T-2 toxin above the maximum permissible concentration (185 mcg/kg). The isolated Fusarium sporotrichioides RM+ isolate had morphological properties typical for this species. Micromycete colonies are fast-growing, abundant aerial mycelium on simple or branched conidiophores. Microconidia pear-shaped, abundant-oval, pointed, 0-1 septum; macroconidia fusiform-Crescent-shaped, fusiform or Crescent-shaped with a gradually narrowing upper cell. The effect of ultraviolet irradiation on the morphological properties and production of a toxic metabolite (T-2 toxin) by the Fusarium sporotrichioidesRM+field isolate was investigated. It is shown that exposure to ultraviolet radiation contributes to changes in the mycelium of microscopic fungi, growth tubes, chlamydospores, the shape and structure of macro and microconidia. After 48 hours of irradiation of the field isolate, chlamydospores bloated and intense division, micro and macroconidia were observed. Exposure of 72 hours contributed to the change of mushroom spores. Long-term ultraviolet irradiation was characterized by mutational changes in the micromycete. The optimal condition for increasing the t-2 toxin content was exposure to UV radiation for 72 hours. The toxin content was 7823.17 mcg / kg, which is 4.2 times higher than the initial level (P<0.001). After 96 hours of ultraviolet irradiation, there was a decrease in toxin formation.

Field isolates of fungi of the genus Fusarium are widely distributed in the environment, producing under certain conditions highly toxic metabolites, in particular T-2 toxin. The aim of the research is to study the effect of ultraviolet irradiation on the production of the t-2 toxin Fusarium sporotrichioides. The experiments were carried out in the laboratory of the Federal State Budgetary Institution "FCTRBS-RRVI". The object of the study was a field isolate Fusarium sporotrichioides RM+, isolated from a sample of feed containing T-2 toxin above the maximum permissible concentration (185 mcg/kg). The isolated Fusarium sporotrichioides RM+ isolate had morphological properties typical for this species. Micromycete colonies are fast-growing, abundant aerial mycelium on simple or branched conidiophores. Microconidia pear-shaped, abundant-oval, pointed, 0-1 septum; macroconidia fusiform-Crescent-shaped, fusiform or Crescent-shaped with a gradually narrowing upper cell. The effect of ultraviolet irradiation on the morphological properties and production of a toxic metabolite (T-2 toxin) by the Fusarium sporotrichioidesRM+field isolate was investigated. It is shown that exposure to ultraviolet radiation contributes to changes in the mycelium of microscopic fungi, growth tubes, chlamydospores, the shape and structure of macro and microconidia. After 48 hours of irradiation of the field isolate, chlamydospores bloated and intense division, micro and macroconidia were observed. Exposure of 72 hours contributed to the change of mushroom spores. Long-term ultraviolet irradiation was characterized by mutational changes in the micromycete. The optimal condition for increasing the t-2 toxin content was exposure to UV radiation for 72 hours. The toxin content was 7823.17 mcg / kg, which is 4.2 times higher than the initial level (P<0.001). After 96 hours of ultraviolet irradiation, there was a decrease in toxin formation.

Field isolates of fungi of the genus Fusarium are widely distributed in the environment, producing under certain conditions highly toxic metabolites, in particular T-2 toxin. The aim of the research is to study the effect of ultraviolet irradiation on the production of the t-2 toxin Fusarium sporotrichioides. The experiments were carried out in the laboratory of the Federal State Budgetary Institution "FCTRBS-RRVI". The object of the study was a field isolate Fusarium sporotrichioides RM+, isolated from a sample of feed containing T-2 toxin above the maximum permissible concentration (185 mcg/kg). The isolated Fusarium sporotrichioides RM+ isolate had morphological properties typical for this species. Micromycete colonies are fast-growing, abundant aerial mycelium on simple or branched conidiophores. Microconidia pear-shaped, abundant-oval, pointed, 0-1 septum; macroconidia fusiform-Crescent-shaped, fusiform or Crescent-shaped with a gradually narrowing upper cell. The effect of ultraviolet irradiation on the morphological properties and production of a toxic metabolite (T-2 toxin) by the Fusarium sporotrichioidesRM+field isolate was investigated. It is shown that exposure to ultraviolet radiation contributes to changes in the mycelium of microscopic fungi, growth tubes, chlamydospores, the shape and structure of macro and microconidia. After 48 hours of irradiation of the field isolate, chlamydospores bloated and intense division, micro and macroconidia were observed. Exposure of 72 hours contributed to the change of mushroom spores. Long-term ultraviolet irradiation was characterized by mutational changes in the micromycete. The optimal condition for increasing the t-2 toxin content was exposure to UV radiation for 72 hours. The toxin content was 7823.17 mcg / kg, which is 4.2 times higher than the initial level (P<0.001). After 96 hours of ultraviolet irradiation, there was a decrease in toxin formation.

Field isolates of fungi of the genus Fusarium are widely distributed in the environment, producing under certain conditions highly toxic metabolites, in particular T-2 toxin. The aim of the research is to study the effect of ultraviolet irradiation on the production of the t-2 toxin Fusarium sporotrichioides. The experiments were carried out in the laboratory of the Federal State Budgetary Institution "FCTRBS-RRVI". The object of the study was a field isolate Fusarium sporotrichioides RM+, isolated from a sample of feed containing T-2 toxin above the maximum permissible concentration (185 mcg/kg). The isolated Fusarium sporotrichioides RM+ isolate had morphological properties typical for this species. Micromycete colonies are fast-growing, abundant aerial mycelium on simple or branched conidiophores. Microconidia pear-shaped, abundant-oval, pointed, 0-1 septum; macroconidia fusiform-Crescent-shaped, fusiform or Crescent-shaped with a gradually narrowing upper cell. The effect of ultraviolet irradiation on the morphological properties and production of a toxic metabolite (T-2 toxin) by the Fusarium sporotrichioidesRM+field isolate was investigated. It is shown that exposure to ultraviolet radiation contributes to changes in the mycelium of microscopic fungi, growth tubes, chlamydospores, the shape and structure of macro and microconidia. After 48 hours of irradiation of the field isolate, chlamydospores bloated and intense division, micro and macroconidia were observed. Exposure of 72 hours contributed to the change of mushroom spores. Long-term ultraviolet irradiation was characterized by mutational changes in the micromycete. The optimal condition for increasing the t-2 toxin content was exposure to UV radiation for 72 hours. The toxin content was 7823.17 mcg / kg, which is 4.2 times higher than the initial level (P<0.001). After 96 hours of ultraviolet irradiation, there was a decrease in toxin formation.

Field isolates of fungi of the genus Fusarium are widely distributed in the environment, producing under certain conditions highly toxic metabolites, in particular T-2 toxin. The aim of the research is to study the effect of ultraviolet irradiation on the production of the t-2 toxin Fusarium sporotrichioides. The experiments were carried out in the laboratory of the Federal State Budgetary Institution "FCTRBS-RRVI". The object of the study was a field isolate Fusarium sporotrichioides RM+, isolated from a sample of feed containing T-2 toxin above the maximum permissible concentration (185 mcg/kg). The isolated Fusarium sporotrichioides RM+ isolate had morphological properties typical for this species. Micromycete colonies are fast-growing, abundant aerial mycelium on simple or branched conidiophores. Microconidia pear-shaped, abundant-oval, pointed, 0-1 septum; macroconidia fusiform-Crescent-shaped, fusiform or Crescent-shaped with a gradually narrowing upper cell. The effect of ultraviolet irradiation on the morphological properties and production of a toxic metabolite (T-2 toxin) by the Fusarium sporotrichioidesRM+field isolate was investigated. It is shown that exposure to ultraviolet radiation contributes to changes in the mycelium of microscopic fungi, growth tubes, chlamydospores, the shape and structure of macro and microconidia. After 48 hours of irradiation of the field isolate, chlamydospores bloated and intense division, micro and macroconidia were observed. Exposure of 72 hours contributed to the change of mushroom spores. Long-term ultraviolet irradiation was characterized by mutational changes in the micromycete. The optimal condition for increasing the t-2 toxin content was exposure to UV radiation for 72 hours. The toxin content was 7823.17 mcg / kg, which is 4.2 times higher than the initial level (P<0.001). After 96 hours of ultraviolet irradiation, there was a decrease in toxin formation.

Field isolates of fungi of the genus Fusarium are widely distributed in the environment, producing under certain conditions highly toxic metabolites, in particular T-2 toxin. The aim of the research is to study the effect of ultraviolet irradiation on the production of the t-2 toxin Fusarium sporotrichioides. The experiments were carried out in the laboratory of the Federal State Budgetary Institution "FCTRBS-RRVI". The object of the study was a field isolate Fusarium sporotrichioides RM+, isolated from a sample of feed containing T-2 toxin above the maximum permissible concentration (185 mcg/kg). The isolated Fusarium sporotrichioides RM+ isolate had morphological properties typical for this species. Micromycete colonies are fast-growing, abundant aerial mycelium on simple or branched conidiophores. Microconidia pear-shaped, abundant-oval, pointed, 0-1 septum; macroconidia fusiform-Crescent-shaped, fusiform or Crescent-shaped with a gradually narrowing upper cell. The effect of ultraviolet irradiation on the morphological properties and production of a toxic metabolite (T-2 toxin) by the Fusarium sporotrichioidesRM+field isolate was investigated. It is shown that exposure to ultraviolet radiation contributes to changes in the mycelium of microscopic fungi, growth tubes, chlamydospores, the shape and structure of macro and microconidia. After 48 hours of irradiation of the field isolate, chlamydospores bloated and intense division, micro and macroconidia were observed. Exposure of 72 hours contributed to the change of mushroom spores. Long-term ultraviolet irradiation was characterized by mutational changes in the micromycete. The optimal condition for increasing the t-2 toxin content was exposure to UV radiation for 72 hours. The toxin content was 7823.17 mcg / kg, which is 4.2 times higher than the initial level (P<0.001). After 96 hours of ultraviolet irradiation, there was a decrease in toxin formation.

Field isolates of fungi of the genus Fusarium are widely distributed in the environment, producing under certain conditions highly toxic metabolites, in particular T-2 toxin. The aim of the research is to study the effect of ultraviolet irradiation on the production of the t-2 toxin Fusarium sporotrichioides. The experiments were carried out in the laboratory of the Federal State Budgetary Institution "FCTRBS-RRVI". The object of the study was a field isolate Fusarium sporotrichioides RM+, isolated from a sample of feed containing T-2 toxin above the maximum permissible concentration (185 mcg/kg). The isolated Fusarium sporotrichioides RM+ isolate had morphological properties typical for this species. Micromycete colonies are fast-growing, abundant aerial mycelium on simple or branched conidiophores. Microconidia pear-shaped, abundant-oval, pointed, 0-1 septum; macroconidia fusiform-Crescent-shaped, fusiform or Crescent-shaped with a gradually narrowing upper cell. The effect of ultraviolet irradiation on the morphological properties and production of a toxic metabolite (T-2 toxin) by the Fusarium sporotrichioidesRM+field isolate was investigated. It is shown that exposure to ultraviolet radiation contributes to changes in the mycelium of microscopic fungi, growth tubes, chlamydospores, the shape and structure of macro and microconidia. After 48 hours of irradiation of the field isolate, chlamydospores bloated and intense division, micro and macroconidia were observed. Exposure of 72 hours contributed to the change of mushroom spores. Long-term ultraviolet irradiation was characterized by mutational changes in the micromycete. The optimal condition for increasing the t-2 toxin content was exposure to UV radiation for 72 hours. The toxin content was 7823.17 mcg / kg, which is 4.2 times higher than the initial level (P<0.001). After 96 hours of ultraviolet irradiation, there was a decrease in toxin formation.

Field isolates of fungi of the genus Fusarium are widely distributed in the environment, producing under certain conditions highly toxic metabolites, in particular T-2 toxin. The aim of the research is to study the effect of ultraviolet irradiation on the production of the t-2 toxin Fusarium sporotrichioides. The experiments were carried out in the laboratory of the Federal State Budgetary Institution "FCTRBS-RRVI". The object of the study was a field isolate Fusarium sporotrichioides RM+, isolated from a sample of feed containing T-2 toxin above the maximum permissible concentration (185 mcg/kg). The isolated Fusarium sporotrichioides RM+ isolate had morphological properties typical for this species. Micromycete colonies are fast-growing, abundant aerial mycelium on simple or branched conidiophores. Microconidia pear-shaped, abundant-oval, pointed, 0-1 septum; macroconidia fusiform-Crescent-shaped, fusiform or Crescent-shaped with a gradually narrowing upper cell. The effect of ultraviolet irradiation on the morphological properties and production of a toxic metabolite (T-2 toxin) by the Fusarium sporotrichioidesRM+field isolate was investigated. It is shown that exposure to ultraviolet radiation contributes to changes in the mycelium of microscopic fungi, growth tubes, chlamydospores, the shape and structure of macro and microconidia. After 48 hours of irradiation of the field isolate, chlamydospores bloated and intense division, micro and macroconidia were observed. Exposure of 72 hours contributed to the change of mushroom spores. Long-term ultraviolet irradiation was characterized by mutational changes in the micromycete. The optimal condition for increasing the t-2 toxin content was exposure to UV radiation for 72 hours. The toxin content was 7823.17 mcg / kg, which is 4.2 times higher than the initial level (P<0.001). After 96 hours of ultraviolet irradiation, there was a decrease in toxin formation.

Field isolates of fungi of the genus Fusarium are widely distributed in the environment, producing under certain conditions highly toxic metabolites, in particular T-2 toxin. The aim of the research is to study the effect of ultraviolet irradiation on the production of the t-2 toxin Fusarium sporotrichioides. The experiments were carried out in the laboratory of the Federal State Budgetary Institution "FCTRBS-RRVI". The object of the study was a field isolate Fusarium sporotrichioides RM+, isolated from a sample of feed containing T-2 toxin above the maximum permissible concentration (185 mcg/kg). The isolated Fusarium sporotrichioides RM+ isolate had morphological properties typical for this species. Micromycete colonies are fast-growing, abundant aerial mycelium on simple or branched conidiophores. Microconidia pear-shaped, abundant-oval, pointed, 0-1 septum; macroconidia fusiform-Crescent-shaped, fusiform or Crescent-shaped with a gradually narrowing upper cell. The effect of ultraviolet irradiation on the morphological properties and production of a toxic metabolite (T-2 toxin) by the Fusarium sporotrichioidesRM+field isolate was investigated. It is shown that exposure to ultraviolet radiation contributes to changes in the mycelium of microscopic fungi, growth tubes, chlamydospores, the shape and structure of macro and microconidia. After 48 hours of irradiation of the field isolate, chlamydospores bloated and intense division, micro and macroconidia were observed. Exposure of 72 hours contributed to the change of mushroom spores. Long-term ultraviolet irradiation was characterized by mutational changes in the micromycete. The optimal condition for increasing the t-2 toxin content was exposure to UV radiation for 72 hours. The toxin content was 7823.17 mcg / kg, which is 4.2 times higher than the initial level (P<0.001). After 96 hours of ultraviolet irradiation, there was a decrease in toxin formation.

Field isolates of fungi of the genus Fusarium are widely distributed in the environment, producing under certain conditions highly toxic metabolites, in particular T-2 toxin. The aim of the research is to study the effect of ultraviolet irradiation on the production of the t-2 toxin Fusarium sporotrichioides. The experiments were carried out in the laboratory of the Federal State Budgetary Institution "FCTRBS-RRVI". The object of the study was a field isolate Fusarium sporotrichioides RM+, isolated from a sample of feed containing T-2 toxin above the maximum permissible concentration (185 mcg/kg). The isolated Fusarium sporotrichioides RM+ isolate had morphological properties typical for this species. Micromycete colonies are fast-growing, abundant aerial mycelium on simple or branched conidiophores. Microconidia pear-shaped, abundant-oval, pointed, 0-1 septum; macroconidia fusiform-Crescent-shaped, fusiform or Crescent-shaped with a gradually narrowing upper cell. The effect of ultraviolet irradiation on the morphological properties and production of a toxic metabolite (T-2 toxin) by the Fusarium sporotrichioidesRM+field isolate was investigated. It is shown that exposure to ultraviolet radiation contributes to changes in the mycelium of microscopic fungi, growth tubes, chlamydospores, the shape and structure of macro and microconidia. After 48 hours of irradiation of the field isolate, chlamydospores bloated and intense division, micro and macroconidia were observed. Exposure of 72 hours contributed to the change of mushroom spores. Long-term ultraviolet irradiation was characterized by mutational changes in the micromycete. The optimal condition for increasing the t-2 toxin content was exposure to UV radiation for 72 hours. The toxin content was 7823.17 mcg / kg, which is 4.2 times higher than the initial level (P<0.001). After 96 hours of ultraviolet irradiation, there was a decrease in toxin formation.

Field isolates of fungi of the genus Fusarium are widely distributed in the environment, producing under certain conditions highly toxic metabolites, in particular T-2 toxin. The aim of the research is to study the effect of ultraviolet irradiation on the production of the t-2 toxin Fusarium sporotrichioides. The experiments were carried out in the laboratory of the Federal State Budgetary Institution "FCTRBS-RRVI". The object of the study was a field isolate Fusarium sporotrichioides RM+, isolated from a sample of feed containing T-2 toxin above the maximum permissible concentration (185 mcg/kg). The isolated Fusarium sporotrichioides RM+ isolate had morphological properties typical for this species. Micromycete colonies are fast-growing, abundant aerial mycelium on simple or branched conidiophores. Microconidia pear-shaped, abundant-oval, pointed, 0-1 septum; macroconidia fusiform-Crescent-shaped, fusiform or Crescent-shaped with a gradually narrowing upper cell. The effect of ultraviolet irradiation on the morphological properties and production of a toxic metabolite (T-2 toxin) by the Fusarium sporotrichioidesRM+field isolate was investigated. It is shown that exposure to ultraviolet radiation contributes to changes in the mycelium of microscopic fungi, growth tubes, chlamydospores, the shape and structure of macro and microconidia. After 48 hours of irradiation of the field isolate, chlamydospores bloated and intense division, micro and macroconidia were observed. Exposure of 72 hours contributed to the change of mushroom spores. Long-term ultraviolet irradiation was characterized by mutational changes in the micromycete. The optimal condition for increasing the t-2 toxin content was exposure to UV radiation for 72 hours. The toxin content was 7823.17 mcg / kg, which is 4.2 times higher than the initial level (P<0.001). After 96 hours of ultraviolet irradiation, there was a decrease in toxin formation.

Field isolates of fungi of the genus Fusarium are widely distributed in the environment, producing under certain conditions highly toxic metabolites, in particular T-2 toxin. The aim of the research is to study the effect of ultraviolet irradiation on the production of the t-2 toxin Fusarium sporotrichioides. The experiments were carried out in the laboratory of the Federal State Budgetary Institution "FCTRBS-RRVI". The object of the study was a field isolate Fusarium sporotrichioides RM+, isolated from a sample of feed containing T-2 toxin above the maximum permissible concentration (185 mcg/kg). The isolated Fusarium sporotrichioides RM+ isolate had morphological properties typical for this species. Micromycete colonies are fast-growing, abundant aerial mycelium on simple or branched conidiophores. Microconidia pear-shaped, abundant-oval, pointed, 0-1 septum; macroconidia fusiform-Crescent-shaped, fusiform or Crescent-shaped with a gradually narrowing upper cell. The effect of ultraviolet irradiation on the morphological properties and production of a toxic metabolite (T-2 toxin) by the Fusarium sporotrichioidesRM+field isolate was investigated. It is shown that exposure to ultraviolet radiation contributes to changes in the mycelium of microscopic fungi, growth tubes, chlamydospores, the shape and structure of macro and microconidia. After 48 hours of irradiation of the field isolate, chlamydospores bloated and intense division, micro and macroconidia were observed. Exposure of 72 hours contributed to the change of mushroom spores. Long-term ultraviolet irradiation was characterized by mutational changes in the micromycete. The optimal condition for increasing the t-2 toxin content was exposure to UV radiation for 72 hours. The toxin content was 7823.17 mcg / kg, which is 4.2 times higher than the initial level (P<0.001). After 96 hours of ultraviolet irradiation, there was a decrease in toxin formation.

Field isolates of fungi of the genus Fusarium are widely distributed in the environment, producing under certain conditions highly toxic metabolites, in particular T-2 toxin. The aim of the research is to study the effect of ultraviolet irradiation on the production of the t-2 toxin Fusarium sporotrichioides. The experiments were carried out in the laboratory of the Federal State Budgetary Institution "FCTRBS-RRVI". The object of the study was a field isolate Fusarium sporotrichioides RM+, isolated from a sample of feed containing T-2 toxin above the maximum permissible concentration (185 mcg/kg). The isolated Fusarium sporotrichioides RM+ isolate had morphological properties typical for this species. Micromycete colonies are fast-growing, abundant aerial mycelium on simple or branched conidiophores. Microconidia pear-shaped, abundant-oval, pointed, 0-1 septum; macroconidia fusiform-Crescent-shaped, fusiform or Crescent-shaped with a gradually narrowing upper cell. The effect of ultraviolet irradiation on the morphological properties and production of a toxic metabolite (T-2 toxin) by the Fusarium sporotrichioidesRM+field isolate was investigated. It is shown that exposure to ultraviolet radiation contributes to changes in the mycelium of microscopic fungi, growth tubes, chlamydospores, the shape and structure of macro and microconidia. After 48 hours of irradiation of the field isolate, chlamydospores bloated and intense division, micro and macroconidia were observed. Exposure of 72 hours contributed to the change of mushroom spores. Long-term ultraviolet irradiation was characterized by mutational changes in the micromycete. The optimal condition for increasing the t-2 toxin content was exposure to UV radiation for 72 hours. The toxin content was 7823.17 mcg / kg, which is 4.2 times higher than the initial level (P<0.001). After 96 hours of ultraviolet irradiation, there was a decrease in toxin formation.

Field isolates of fungi of the genus Fusarium are widely distributed in the environment, producing under certain conditions highly toxic metabolites, in particular T-2 toxin. The aim of the research is to study the effect of ultraviolet irradiation on the production of the t-2 toxin Fusarium sporotrichioides. The experiments were carried out in the laboratory of the Federal State Budgetary Institution "FCTRBS-RRVI". The object of the study was a field isolate Fusarium sporotrichioides RM+, isolated from a sample of feed containing T-2 toxin above the maximum permissible concentration (185 mcg/kg). The isolated Fusarium sporotrichioides RM+ isolate had morphological properties typical for this species. Micromycete colonies are fast-growing, abundant aerial mycelium on simple or branched conidiophores. Microconidia pear-shaped, abundant-oval, pointed, 0-1 septum; macroconidia fusiform-Crescent-shaped, fusiform or Crescent-shaped with a gradually narrowing upper cell. The effect of ultraviolet irradiation on the morphological properties and production of a toxic metabolite (T-2 toxin) by the Fusarium sporotrichioidesRM+field isolate was investigated. It is shown that exposure to ultraviolet radiation contributes to changes in the mycelium of microscopic fungi, growth tubes, chlamydospores, the shape and structure of macro and microconidia. After 48 hours of irradiation of the field isolate, chlamydospores bloated and intense division, micro and macroconidia were observed. Exposure of 72 hours contributed to the change of mushroom spores. Long-term ultraviolet irradiation was characterized by mutational changes in the micromycete. The optimal condition for increasing the t-2 toxin content was exposure to UV radiation for 72 hours. The toxin content was 7823.17 mcg / kg, which is 4.2 times higher than the initial level (P<0.001). After 96 hours of ultraviolet irradiation, there was a decrease in toxin formation.

Field isolates of fungi of the genus Fusarium are widely distributed in the environment, producing under certain conditions highly toxic metabolites, in particular T-2 toxin. The aim of the research is to study the effect of ultraviolet irradiation on the production of the t-2 toxin Fusarium sporotrichioides. The experiments were carried out in the laboratory of the Federal State Budgetary Institution "FCTRBS-RRVI". The object of the study was a field isolate Fusarium sporotrichioides RM+, isolated from a sample of feed containing T-2 toxin above the maximum permissible concentration (185 mcg/kg). The isolated Fusarium sporotrichioides RM+ isolate had morphological properties typical for this species. Micromycete colonies are fast-growing, abundant aerial mycelium on simple or branched conidiophores. Microconidia pear-shaped, abundant-oval, pointed, 0-1 septum; macroconidia fusiform-Crescent-shaped, fusiform or Crescent-shaped with a gradually narrowing upper cell. The effect of ultraviolet irradiation on the morphological properties and production of a toxic metabolite (T-2 toxin) by the Fusarium sporotrichioidesRM+field isolate was investigated. It is shown that exposure to ultraviolet radiation contributes to changes in the mycelium of microscopic fungi, growth tubes, chlamydospores, the shape and structure of macro and microconidia. After 48 hours of irradiation of the field isolate, chlamydospores bloated and intense division, micro and macroconidia were observed. Exposure of 72 hours contributed to the change of mushroom spores. Long-term ultraviolet irradiation was characterized by mutational changes in the micromycete. The optimal condition for increasing the t-2 toxin content was exposure to UV radiation for 72 hours. The toxin content was 7823.17 mcg / kg, which is 4.2 times higher than the initial level (P<0.001). After 96 hours of ultraviolet irradiation, there was a decrease in toxin formation.

Field isolates of fungi of the genus Fusarium are widely distributed in the environment, producing under certain conditions highly toxic metabolites, in particular T-2 toxin. The aim of the research is to study the effect of ultraviolet irradiation on the production of the t-2 toxin Fusarium sporotrichioides. The experiments were carried out in the laboratory of the Federal State Budgetary Institution "FCTRBS-RRVI". The object of the study was a field isolate Fusarium sporotrichioides RM+, isolated from a sample of feed containing T-2 toxin above the maximum permissible concentration (185 mcg/kg). The isolated Fusarium sporotrichioides RM+ isolate had morphological properties typical for this species. Micromycete colonies are fast-growing, abundant aerial mycelium on simple or branched conidiophores. Microconidia pear-shaped, abundant-oval, pointed, 0-1 septum; macroconidia fusiform-Crescent-shaped, fusiform or Crescent-shaped with a gradually narrowing upper cell. The effect of ultraviolet irradiation on the morphological properties and production of a toxic metabolite (T-2 toxin) by the Fusarium sporotrichioidesRM+field isolate was investigated. It is shown that exposure to ultraviolet radiation contributes to changes in the mycelium of microscopic fungi, growth tubes, chlamydospores, the shape and structure of macro and microconidia. After 48 hours of irradiation of the field isolate, chlamydospores bloated and intense division, micro and macroconidia were observed. Exposure of 72 hours contributed to the change of mushroom spores. Long-term ultraviolet irradiation was characterized by mutational changes in the micromycete. The optimal condition for increasing the t-2 toxin content was exposure to UV radiation for 72 hours. The toxin content was 7823.17 mcg / kg, which is 4.2 times higher than the initial level (P<0.001). After 96 hours of ultraviolet irradiation, there was a decrease in toxin formation.

  Radiobiology
   
Title:

EXPERIMENTAL SELECTION OF DOSES OF IONIZING RADIATION CAUSING GROWTH INHIBITION AND FULL INACTIVATION OF GOLDEN STAFILOKOK

   
Authors: Gaynutdinov T.R. - andidate of Biological Sciences
   
Address:

FSBSI Federal Center for Toxicological, Radiation and Biological Safety,

(420075, Kazan, Nauchny gorodok-2, e-mail: vnivi@mail.ru)

   
Abstract:

Experiments to study the radioresistance of Staphylococcus aureus culture were carried out in the Department of Radiobiology of the Federal State Budgetary Scientific Institution FCTRBS-RRVI. Gamma irradiation of the test material was carried out on the Researcher installation with a 60Co radiation source and an exposure dose of 3.7 kGy/hour, at doses of 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70 kGy . The degree of inactivation of gamma-irradiated microorganisms was determined by plating them on Kitt-Tarozzi medium with the addition of cattle serum and salt meat and peptone agar, followed by incubation for 7 days and recording the presence or absence of microorganism growth. Studies have shown that gamma irradiation of a suspension of Staphylococcus aureus microorganisms in dose ranges from 20 to 65 kGy causes growth retardation, complete inactivation of the culture occurs when irradiated at a dose of 70 kGy.

  Biotechnology
   
Title:

DETERMINATION OF SPECIFIC ANTIBODIES TO MORAXELLA BOVOCULI BACTERIA IN BLOOD SERUM OF CATTLE BY ELISA

   
Authors: Dupleva L.Sh. - Candidate of Biological Sciences, Spiridonov G.N. - Doctor of Biological Sciences, Khusainov I.T. - junior research.
   
Address:

FSBSI Federal Center for Toxicological, Radiation and Biological Safety,

(420075, Kazan, Nauchny gorodok-2, e-mail: vnivi@mail.ru)

   
Abstract:

The results of a study on the creation of an enzyme immunoassay system for the serological diagnosis of infectious keratoconjunctivitis in cattle and the control of post-vaccination immunity are presented. The antigen made from the SH-CH6  DEP   strain of Mraxella bovoculi bacteria by destroying bacterial cells on an ultrasonic disintegrator, as well as cattle serum positive and negative for Moraxella bovoculi bacteria, were used as the main components in the enzyme immunoassay system. According to the results of chess titration of antigen, control sera and peroxidase conjugate, their optimal working doses were established. A laboratory test of the specificity and sensitivity of the enzyme-linked immunosorbent assay system was carried out. As a result of studies, it was found that all components of the kit are sensitive, specific and active. Using the Drug Kit for the Detection of Specific Antibodies to Moraxella bovoculi Bacteria by Enzyme Immunoassay (ELISA), a study was carried out of blood serum samples from cattle from various epizootic infectious keratoconjunctivitis cattle farms in the Republic of Tatarstan. An enzyme-linked immunosorbent assay system was found to detect specific antibodies to Moraxella bovoculi bacteria in 97,3% of healthy vaccinated and 95,8% of patients with infectious keratoconjunctivitis animals. At the same time, it was found that the components of the kit do not react with blood sera obtained from intact animals from farms that are successful in infectious keratoconjunctivitis. Thus, the proposed test system allows the titer of specific antibodies in the blood serum to identify sick animals among unvaccinated livestock and determine the intensity of immunity in vaccinated animals. Based on the results of studies on the enzyme immunoassay test system, regulatory documents for its manufacture, control and use were developed, and a RF patent was obtained.

   
Keywords: Keywords: infectious keratoconjunctivitis, bacteria Moraxella bovoculi, cattle, antigen, enzyme-linked immunosorbent assay.
   
References:

References 

1.Vasilev, D.A. Sovremennyie metodyi immunodiagnostiki infektsionnyih bolezney [Modern methods of immunodiagnostics of infectious diseases] / D.A. Vasilev, P.I. Baryishnikov, B.V. Novikov /  Ulyanovsk. - 1998. - . 15.

2.Gaffarov, H.Z. Immunofermentnyiy analiz v diagnostike reovirusnoy infektsii krupnogo rogatogo skota [Enzyme-linked immunosorbent assay for the diagnosis of rheovirus infection in cattle] / H.Z. Gaffarov, M.A. Efimova, O.V. Moskvichev // Veterinarnyiy vrach. - 2011. - 5. - . 14.

3.Karaychentsev, D.V. Sovershenstvovanie laboratornoy diagnostiki infektsionnogo keratokon'yunktivita krupnogo rogatogo skota: avtoref. dis. k. vet. nauk. [Improving the laboratory diagnosis of infectious keratoconjunctivitis in cattle] / D.V. Karaychentsev / M. - 2016. - 23 .

4.         Patent 2521651 Ros. Federatsiya, MPK S12N 1/20, A61K 39/00. Shtamm bakteriy Moraxella bovoculi SH-CH6  DEP, ispolzuemyiy dlya izgotovleniya diagnostikumov i vaktsin protiv infektsionnogo keratokonyunktivita krupnogo rogatogo skota [The bacterial strain Moraxella bovoculi  SH-CH6  DEP used for the manufacture of diagnosticums and vaccines against infectious keratoconjunctivitis in cattle] / A.V. Ivanov, G.N. Spiridonov, A.A. Ivanov [i dr.]; zayavitel FGBU FTsTRB-VNIVI. - 2013117096/10; opubl. 10.07.2014, Byul. 19.

5.         Spiridonov, A.G. Immunofermentnyiy metod diagnostiki anaerobnoy enterotoksemii zhivotnyih [Enzyme immunoassay for the diagnosis of animal anaerobic enterotoxemia] / A.G. Spiridonov // Veterinarnyiy vrach. - Kazan. - 2018. - 6. - . 26.

6. Spiridonov, G.N. Biologicheskie svoystva bakteriy Moraxella bovoculi - vozbuditelya infektsionnogo keratokonyunktivita krupnogo rogatogo skota [Biological properties of bacteria Moraxella bovoculi - the causative agent of infectious keratoconjunctivitis in cattle] / G.N. Spiridonov, L.V. Valebnaya, L.Sh. Dupleva [i dr.] // Veterinarnyiy vrach. - 2017. - 3. - .  8.

7. Angelos, J.A. Cloning and characterization of a Moraxella bovis cytotoxin gene / J.A. Angelos, J.F. Hess, L.W. George // Am. J. Vet. Res. - 2001. - V. 62. - P. 1222.

8. Angelos, J.A. Differentiation of Moraxella bovoculi sp. nov. from other coccoid moraxellae by the use of polymerase chain reaction and restriction endonuclease analysis of amplified DNA / J.A. Angelos,        L.M. Ball // J. Vet. Diagn. Invest. - 2007. - V. 19 - P. 532.

9. Angelos, J.A Recombinant Moraxella bovoculi cytotoxin-ISCOM matrix adjuvanted vaccine to prevent naturally occurring infectious bovine keratoconjunctivitis / J.A. Angelos, V.M. Lane, L.M. Ball,             J. F. Hess // Vet. Res. Commun. - 2010. - V. 34(3). - P. 229.

10.       Bishop, B. Enzyme - linked immunosorbent assay for measurement of anti - Moraxella bovis antibodies / B. Bishop, G.G. Schurig, H.F. Troutt // Am. J. Vet. Res. - 1982. - V. 43. - P. 1443.

11.       Galvão, K.N. Ulcerative blepharitis and conjunctivitis in adult dairy cows and association with Moraxella bovoculi / N. K. Galvão, J. A. Angelos // Can Vet J. - 2010. - V. 51(4). - P. 400.

12.       Lapper, A.W.D. Vaccination against infectious bovine keratoconjunctivitis: protective efficacy and antibody response induced by by pili of homologous und heterologous Strains of Moraxella bovis / A.W.D. Lapper. // Austr. Vet. J.  - 1988. - 65-10- P. 129.

13.       McConnel, C.S. Serologic cross-reactivity of Australian Moraxella bovis to vaccinal bacterin trains as determined by competitive ELISA / C.S McConnel, L. Shum, B.L. Gleeson, J.K. House // J. Aust. Vet. 2008. -V.86.- P. 124 -129.

14.       Postma, G.C. Moraxella bovis pathogenicity: an update / G.C. Postma, J.C. Carfagnini,           L. Minatel // Comp. Immunol. Microbiol. Infect. Dis. 2008. - V. 31 (6). - P. 449.

 

  VETERINARY PHARMACOLOGY WITH TOXICOLOGY
   
Title:

COMPLEX CONIFEROUS BALSAMIC FIR FOR DISINFECTION OF HATCHING EGGS

   
Authors: Suntsova O.A. - Candidate of Veterinary Sciences, Zadorozhnaya M.V. - Candidate of Veterinary Sciences, Lysko S.B. - Candidate of Veterinary Sciences
   
Address:

Siberian Research Institute of Poultry Farming is a branch of

the Federal State Budgetary Scientific Institution "Omsk Agricultural Research Center"

(644555, Omsk Region, Omsk District, p. Morozovka,

st. 60 years of Victory, 1, sibniip@mail.ru)

   
Abstract:

In the technological process of poultry production, the most vulnerable is the incubator, in which favorable conditions are created not only for the developing embryo, but also for microorganisms. The task of disinfection is to prevent the accumulation of microflora to critical levels and to prevent overgrowth of young animals at the conclusion. However, not all products used to process hatching eggs are effective and safe for future offspring, staff and the environment. The aim of the study was to develop an effective scheme for the disinfection of hatching eggs using a complex of coniferous balsamic fir. The studies were carried out in the Department of Veterinary Poultry Siberian Research Institute of poultry farming - is a branch of the Federal State Budgetary Scientific Institution "Omsk Agricultural Research Center" and in the poultry farm on incubatory eggs of the quail of the Pharaon breed. In experiments in vitro, it was found that the optimal effect on microorganisms is a 10% aqueous solution of the complex coniferous balsamic fir for 60 minutes, which caused the death of all test cultures. When studying different patterns of application of the test drug under experimental conditions, double-disinfection of incubatory eggs was found to be most effective, which reduces insemination by 31.4-75.0%, the frequency of isolation of staphylococci and enterococci by 20-40%, enterobacteria by 20%, which increases the hatchability of eggs by 4.0% and young hatching by 3.7%. Testing this circuit in a production experiment confirmed the experimental data. Processing incubation eggs of quail eggs with a 10% aqueous solution of a complex of coniferous balsamic fir before incubation and for 15.5 days reduces the microbial insemination of the shell by 41.5-90.5%, increases the hatchability of eggs by 3.9%, the output is quail by 2.7%, the safety of hatched young quails for the first 14 days of life by 2% does not have a negative effect on both the developing embryo and the maintenance staff and can be recommended for use in poultry farms.

   
Keywords: complex coniferous balsamic fir, microbial contamination, incubatory egg of the quail, hatchability of eggs, young hatching, egg disinfection
   
References:

References

1. Bushina O.A. Primeneniye nekotorykh sovremennykh khimicheskikh preparatov dlya dezinfektsii inkubatsionnykh yaits kur [The use of some modern chemicals for the disinfection of hatching chicken eggs] / O.A. Bushina // Veterinarnaya meditsina. - 2009. - Vol. 1-2. - P. 90.

2. Donskov A.P. Sposoby dezinfektsii inkubatsionnykh yaits  [Methods for disinfection of hatching eggs] / A.P. Donskov, D.D. Krivchik, A.P. Voloshin // Mezhdunar. nauch. periodich. izd. po itogam Mezhdunar. nauch.-praktich. konf.: Novaya nauka: strategii i vektory razvitiya : v 2 ch. Sterlitamak: RITS AMI, 2016. - Part 1. - P. 9.

3. Zadorozhnaya, M. Laboratornyye ispytaniya novykh rastitelnykh preparatov dlya obezzarazhivaniya ob"yektov ptitsevodstva [Laboratory tests of new herbal preparations for disinfection of poultry facilities] / M. Zadorozhnaya, S. Lysko, A. Portyanko i dr. // Glavnyy zootekhnik. - 2019. - Vol. 9. - P. 9.

4. Zelenskaya, K.L. Uglekislotnyye ekstrakty pikhty i spirtovyye ekstrakty delfiniuma i boligolova v kompleksnom lechenii i profilaktike vospalitel'nykh zabolevaniy verkhnikh dykhatelnykh putey  [Carbon dioxide extracts of fir and alcoholic extracts of delphinium and hemlock in the complex treatment and prevention of inflammatory diseases of the upper respiratory tract] / K.L. Zelenskaya, YU.V. Nesterova, T.N. Povetyeva i dr. // Sovremennyye problemy fitoterapii: Mat. I Mezhdunar. s"yezda fitoterapevtov. - M., 2006. - P. 84.

5. Krasnobayev, YU. Dezinfektsiya inkubatsionnykh yaits [Disinfection of hatching eggs ] /                     Y.U. Krasnobayev, O. Krasnobayeva, A. Krykanov i dr. // Ptitsevodstvo. - 2011. - Vol. 9. - P. 63.

6. Krasnobayeva, O.A. Na chto sleduyet obratitvnimaniye pri dezinfektsii inkubatsionnykh yaits [What should I look for when disinfecting hatching eggs] / O.A. Krasnobayeva, YU.V. Krasnobayev,             L.P. Gontsova i dr. // Veterinariya Kubani. - 2012. - Vol. 4. - P. 13.

7. Kulikova A.V. Vliyaniye pikhtovita na produktivnosti antioksidantnyy status broylero [The effect of fir on the productivity and antioxidant status of broilers] / A.V. Kulikova, A.V. Khokhlova // Veterinariya. - 2007. - Vol. 2. - P. 12-15.

8. Lakin, G.F. Biometriya  [Biometry] / G.F. Lakin. - M.: Vysshaya shkola, 1973. - 343 p.

9. Maymeskulova L.A. Issledovaniye nekotorykh vidov biologicheskoy aktivnosti izvlecheniy iz pikhty [Elektronnyy resurs] [The study of some types of biological activity of extracts from fir] /                          L.A. Maymeskulova, I.M. Koshkarev, V.I. Karpitskiy. - (https://cow-leech.ru/docs/index-8264.html)

10. Metodicheskiye rekomendatsii po inkubatsii yaits selskokhozyaystvennoy ptitsy . [Guidelines for the incubation of eggs of poultry] Sergiyev Posad: FGBNU VNITIP, 2011. 46 p.

11. Metodicheskiye ukazaniya o poryadke ispytaniya novykh dezinfitsiruyushchikh sredstv dlya veterinarnoy praktiki  [Guidelines on the test procedure for new disinfectants for veterinary practice ] / Gosagroprom SSSR. M., 1987 45 p.

12. Polyanchikov A.A. Populyatsionnaya genetika v ptitsevodstve  [Population genetics in poultry farming] / A.A. Polyanchikov. - M.: Kolos, 1980. - 44 p.

13. Pravila provedeniya dezinfektsii i dezinvazii ob"yektov veterinarnogo nadzora [Tekst] [Rules for the disinfection and disinfestation of objects of veterinary surveillance]: 13-5-2/0525 Utv. 15.07.2002

14. Smirnov A.A. Eksperimental'nyye issledovaniya po dezinfektsii ozonom perepelinykh inkubatsionnykh yaits [Tekst] [Experimental studies on ozone disinfection of quail hatching eggs] / A.A. Smirnov // Innovatsii v sel'skom khozyaystve. - 2015. - Vol. 5 (15). - P. 53-55.

15. Khvoynyye substantsii dlya sel'skogo khozyaystva [Elektronnyy resurs] [Coniferous substances for agriculture]. / - (http://solagift.ru/?page_id=403)

  VETERINARY VIROLOGY, EPIZOOTOLOGY AND ANIMAL THERAPY
   
Title:

ELISA METHOD FOR DETERMINING SPECIFIC ANTIBODIES TO MYCOBACTERIUM BOVIS IN BLOOD SERUM OF CATTLE

   
Authors: 1Spiridonov G.N. - Doctor of Biological Sciences, 1Gaffarov Kh.Z. - Doctor of Veterinary Sciences, Professor, 2Gilmutdinov R.Ya. - Doctor of Biological Sciences, Professor, 1Spiridonov A.G. - Candidate of Biological Sciences.
   
Address:

1 FSBSI Federal Center for Toxicological, Radiation and Biological Safety,

(420075, Kazan, Nauchny gorodok-2,  e-mail: vnivi@mail.ru)

 

2 Kazan state Academy of veterinary medicine Bauman Institute of medicine,

(420074, Kazan, Sibirskiy trakt 35,)


   
Abstract:

The research presented the results of a study on the development of an enzyme immunoassay system for determining specific antibodies to Mycobacterium bovis. The main components of the immunoassay test system are an antigen obtained from the BCG vaccine strain by water-phenol extraction, as well as positive and negative bovine serum for Mycobacterium bovis bacteria. Positive serum was obtained on clinically healthy producing bulls by hyperimmunizing them with a BCG vaccine strain. We conducted a production test of an enzyme immunoassay system to assess the epizootic situation in           6 dysfunctional farms for bovine tuberculosis. Studies have found that up to 92.7% of cows that respond positively to tuberculin from tuberculosis-affected farms give a positive reaction in the ELISA. The titers of anti-TB antibodies in their blood serum ranged from 1: 120 to 1: 7680. During post-slaughter examination, 18-55% of animals showed changes in internal parenchymal organs and regional lymph nodes characteristic of tuberculosis. During the bacteriological study of pathological material from these animals, virulent isolates of Mycobacterium bovis were isolated. We conducted an ELISA study of 58 samples of blood serums obtained from cows that responded positively to tuberculin from tuberculosis-free farms. At the same time, 54 (93.1%) of them gave a negative reaction, 4 samples (6.9%) a positive reaction in dilutions of serums 1:40 1:80. Post-mortem examination of internal parenchymal organs and regional lymph nodes did not reveal changes characteristic of tuberculosis. 8 cows from the private sector that respond positively to tuberculin were slaughtered. During post-slaughter examination, 6 heads were found to have dicroceliosis. Bacteriological studies of parenchymal organs and lymph nodes, serological studies of blood serums in ELISA from all 8 animals gave negative results. The results of the research allow us to assert the possibility of using ELISA for the lifetime diagnosis of bovine tuberculosis.

   
Keywords: tuberculosis, cattle, diagnostics, antigen, Mycobacterium bovis, enzyme immunoassay References
   
References:

1. Arakelov, A.B. Polucheniye antigena MRV83 Mycobacterium bovis dlya diagnostiki tuberkuleza zhivotnykh metodom IFA: dis. kand. biol. nauk /A.B. Arakelov. M., 2006. P. 152.

2. Ivanov, A.V. Razrabotka i ispytanie test-sistemy dlya diagnostiki hlamidioza krupnogo rogatogo skota metodom IFA / A.V. Ivanov, V.V. Evstifeev, F.M. Husainov i dr. // Veterinarnyj vrach. - 2011. - 2. -  P. 10.

3. Kosarev, M.A. Differentsial'naya serologicheskaya diagnostika brutselleza u krupnogo rogatogo skota, privitogo vaktsinoy iz shtamma 82, i yeye znacheniye v obshchey sisteme mer bor'by s dannym zabolevaniyem / M.A. Kosarev, A.M. Fomin, G.M. Safina i dr. // Veterinarnyy vrach. - 2019. - 5. - P. 23.

4. Metodicheskiye rekomendatsii po differentsial'noy diagnostike nespetsificheskikh reaktsiy u krupnogo rogatogo skota pri diagnosticheskom issledovanii na tuberkulez / A. T. Tatarchuk [i dr.]. - Yekaterinburg: IRA UTK, 2004. P. 24.

5. Naymanov, A. KH. Mikobakterialnyye infektsii krupnogo rogatogo skota / A.KH. Naymanov, M.I. Gulyukin. - M.: Zoovetkniga, 2014. P. 235.

6. Patent RF 2416428, MPK A61K39/00 S12N1/20. Sposob polucheniya antigena dlya diagnostiki tuberkuleza krupnogo rogatogo skota / T.R. Yakupov, K.S. Khayertdinov, R.A. Khamzin, I.K. Fakhrtdinov; zayavitel i patentoobladatel FGBOU VPO KGAVM im. N.E. Baumana; opubl. 20.04.2011.

7. Spiridonov, A.G. Immunofermentnyy metod diagnostiki anaerobnoy enterotoksemii zhivotnykh / A.G. Spiridonov // Veterinarnyy vrach. - Kazan'. - 2018. - 6. - P. 26.

8. Khazipov, N.Z. Sposob polucheniya antigena dlya diagnostiki tuberkuleza krupnogo rogatogo skota / N.Z. Khazipov, A.A. Nurullin, V.P. Koksin, R.P. Tyurikova // Avtorskoye svidetelstvo 1638853, 1990.

9. Khamzin, R.A. Immunofermentnyy analiz dlya vyyavleniya protivotuberkuleznykh antitel u krupnogo rogatogo skota /R.A. Khamzin, M.A. Safin, K.G. Idrisova // Voprosy borby s tuberkulezom lyudey i zhivotnykh. Tashkent, 1990. P. 112-114.

10. Khodun, L.M. Test-sistema Elisa dlya diagnostiki tuberkuleza krupnogo rogatogo skota i kriterii otsenki rezul'tatov metoda / L.M. Khodun, N.I. Tsunskaya // Razrabotka sredstv i metodov borby s tuberkulezom zhivotnykh. Novosibirsk, 1990. P. 10.

11. Tsunskaya, N.I. Diagnostika tuberkuleza krupnogo rogatogo skota metodom IFA / N.I. Tsunskaya, L.T. Appelgants // Sistema mer borby s tuberkulezom s.-kh. zhivotnykh. Novosibirsk, 1991. P. 35.

12. Moudgil, K.D. Evaluation of an enzyme immunoassay based on sonicate supernation antigens of Mycobacterium W for immunodiagnostic of leprosy / K.D. Moudgil, S.K. Gupta // Indian J. Lepr. 1988. 60. - 2. P. 159.

13. Ritacco, V. Further evaluation of an indirect enzyme-linked immunosorpent assay for the diagnosis of bovine tuberculosis / V. Ritacco, B. Lopez, L. Barrera / J. Veter. Med. Ser. B. 1990, 37. P. 19.

14. Tacco, R. Assessment of the sensitivitiand specificity of indirect enzyme-linked immunosorpent / R. Tacco, V. Kantor / J. Veter. Med. Ser. B. 1987, vol. 34. P. 119.

15. Immunofermentnyy analiz krovi pri tuberkuleze. https://siestafit.ru/analiz-krovi/immunofermentnyy-analiz-krovi-pri-tuberkuleze.

16. IFA na tuberkulez ili immunofermentnyy analiz na tuberkulez http://tbdoc.ru/analysis/krovi.html.

17. Patomorfologicheskiye izmeneniya v organizme zhivotnykh pri tuberkuleze https://studfiles.net/preview/2901312/page:2.

18. Tuberkulez. https://vetvo.ru/tuberkulez.html.

19.Tuberkulez. https://nsau.edu.ru/images/vetfac/images/ebooks/microbiology/stu/micro/tuberk.htm.

20. Osobennosti provedeniya testa IFA na tuberkulez. https://pulmonologi.ru/tuberkulez/ifa.html.

   
   
Title:

METHODS OF COMBATING RABIES IN WILD ANIMALS USING SMALL AIRCRAFT.

   
Authors: Melnik R.N. - candidate of biological sciences
   
Address:

Federal State Budgetary Institution All-Russian Scientific Research

and Technological Institute of Biological Industry

(141142, Moscow Region, Biocombinat, e-mail: vnitibp@mail.ru)

   
Abstract:

The article describes the state of the country's wild fauna in relation to rabies of animals (especially wild carnivores), which is one of the most important international criteria (WHO, FAO) for assessing the biological and environmental safety of a human environment. Due to the natural and territorial features of Russia, measures to improve the territory from rabies should be carried out on the basis of our own program, taking into account the experience and performing culture of the European society. In this situation, in order to avoid the extremely serious consequences associated with the further spread of rabies in the country, it is urgently necessary: a) Improving and creating new drugs and methods; b) Organization of production of a new generation of vaccines; c) Organization of transportation and storage of drugs; d) Organization of unfolding measures (in all possible ways: manual layout, auto-moto layout, layout on snowmobiles, aviation layout-scattering) bait with a vaccine for oral vaccination of wild carnivores; e) Organization of the production of test systems for monitoring the rabies of wild carnivores, unproductive and farm animals; f) To draw the attention of the leaders of the constituent entities of the Russian Federation, especially in regions of extremely unfavorable rabies, to the social and international significance of this problem.

The rise and decrease in the number of cases of rabies in our country is closely related to the volume of government procurement of drugs for the prevention of rabies and their local use. We have developed a formulation of a pressed solid form of bait, which did not collapse when thrown from a height, and was stored without signs of bacterial growth for 30 days at a temperature of 13-15 C (observation period). The briquette bait for wild animals, includes a vaccine in a polypropylene shell. The aviation method for distributing vaccine decoys is used in most European countries. The quality of the spread of baits when using aviation is controlled by a computer and a satellite navigation system (GPS or equivalent), which allows you to accurately document the coordinates and frequency of the baits discharge. To increase the efficiency and automation of the spread of rabies vaccine baits over large areas, we developed and manufactured a model of a universal aviation bait spreader. The control unit software includes a spreader control program (depending on the flight characteristics of the aircraft or unmanned aerial vehicle), as well as a report program that includes marking the bait unloading points by GPS coordinates attached to the terrain and generating a report on the flight results indicating discharge points, which is controlled by an LED bait unloading control system. The number of vaccine baits per 1 km is 25-30 baits / km2. [2,3,5].

   
Keywords: rabies, small aircraft, vaccine, drones, bait, satellite navigation, oral immunization.
   
References:

References

1. Melnik, N.V. The current state and development trends of animal husbandry in the Moscow region / N.V. Melnik, A.Ya. Samuilenko, M.G. Vinnikov and others // Veterinary medicine. - 2019. - No. 2. - p. 3.

2. Patent PF No. 187545 Spreader / N.V. Melnik, A.Ya. Samuilenko, S.A. Grin et al. 03/12/2019.

3. Patent PF No. 184699 Spreader / N.V. Melnik, A.Ya. Samuilenko, S.A. Grin et al., November 6, 2018.

4. Klyukina, V.I. Genetic engineering plant vaccines against rabies / materials of the international scientific-practical conference "Scientific basis for the production and quality assurance of biological products for the agricultural sector". - Schelkovo. - 2014 .- P. 385.

5. Pukhova N.M. Methods of combating rabies in carnivores / N.M. Pukhova, A.Ya. Samuilenko, N.K. Eremets // Veterinary medicine and feeding. 2014 -. No. 4. - p. 48.

6. Patent RF No. 2538617, The method of obtaining rabies vaccines / A.Ya. Samuilenko, N.I. Zenov and etc. 05.21.2014.

7. Patent RF No. 2287343The method of obtaining rabies vaccines / N.V. Melnik, N.I. Zenov,  S.N. Krasutkin and etc., 05.31.2005.

  GENETICS OF FARM ANIMALS
   
Title:

PRODUCTIVITY, MORPHOMETRIC AND INCUBATION QUALITIES OF EGGS OF LAYING HENS WHEN USING AN ANTIOXIDANT SUPPLEMENT IN THEIR FEEDING (PRODUCTION EXPERIENCE)

   
Authors: Pykhtina L.A. - Doctor of Agricultural Sciences, Professor, Ulitko V.E. - Doctor of Agricultural Sciences, Professor, Gulyaeva L.Yu. - Candidate of Agricultural Sciences, Savina E.V. - Candidate of Agricultural Sciences, Desyatov O.A. - Candidate of Agricultural Sciences, Associate, Semenova Yu.V - Candidate of Agricultural Sciences
   
Address:

Federal State Budgetary Educational Institution of Higher Professional Education

 Ulyanovsk State Agricultural University named after P.A. Stolypin

(432017, 1 Novy Venets Boulevard, e-mail: krmlen@yandex.ru)

   
Abstract:

The article experimentally substantiates the feasibility of feeding in industrial technology conditions to the young of the parent herd and laying hens of compound feed enriched with the antioxidant drug "Carcesel". It was found that it, along with a positive effect on the growth of repair young, significantly (P<0.01-0.001) affects the growth of its reproductive system, which was manifested in an increase in ovarian mass by 11.40%, weight and length of the oviduct by 16.36 and 38.02%, respectively, in relation to the poultry population of the control group. Such differences undoubtedly affected the quantitative and qualitative indicators of their egg productivity, and, consequently, significantly caused an increase in the yield of hatching and fertilized eggs. Feeding the vitamin-selenium-containing preparation "Carcesel" in the poultry diet causes its better survival and a higher level of manifestation of the potential of egg productivity and morpho biochemical composition of eggs, which is manifested in the best of their incubation qualities: fertilization, hatchability and Hatchling. Feed conversion and other economic indicators of rearing young and egg production are also improving.

The research was carried out with the financial support of the RFBR and the Ulyanovsk region in the framework of scientific project No. 19-416-730005.

   
Keywords: antioxidant preparation, egg production, morphological and biochemical parameters of eggs, reproductive organs.
   
References:

References

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Title:

DYNAMICS OF GROWTH AND DEVELOPMENT OF GLOWS DEPENDING ON THE NUMBER OF ASPARAGINATES IN THEIR DIES

   
Authors: Ziruk I.V. - andidate of Veterinary Sciences, Associate Professor; Kopchekchi M.E. - Candidate of Veterinary Sciences, Associate Professor; Egunova A.V. - Candidate of Biological Sciences, Associate Professor; Skliarov S.P. - Candidate of Veterinary Sciences; Miroshnichenko P.V. - Candidate of Veterinary Sciences
   
Address:

Saratov state agrarian University Vavilov University, Saratov

(410012, Saratov, Teatralnaya Square, 1, -mail: iziruk@yandex.ru)

   
Abstract:

Minerals, including trace elements, occupy an important place in ensuring proper animal feeding. It is important to keep in mind that mineral additives in the form of inorganic salts such as sulfates or oxides of various metals are poorly absorbed by the body. At the same time, the absorption of salts of organic amino acids, which are more similar in structure to a living cell, occurs in a much larger volume indicate that many organic forms of trace elements are more accessible than inorganic. The analysis is carried out and the effect of a different number of doses of a complex of trace elements based on L-aspartic acid on the dynamics of growth and development of gilts is studied. It was revealed that the complex used throughout the experimental period contributed to a positive trend in increasing the average live weight, organometrical indices of the internal organs, which was most clearly observed in gilts of the 2nd experimental group, which received 10% of the microelement complex based on L-aspartic acid in the diet . Therefore, the inclusion in the diet of 10% of the generally accepted norm of a complex of trace elements (zinc, iron, copper, cobalt and manganese) in connection with L-aspartic acid, was sufficient to provide the body of the gilts of the 2nd experimental group with higher protective properties, compared to their peers experience.

   
Keywords: stomach, pigs, live weight, trace elements, dynamics.
   
References:

References

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Title:

THE EXAMINATION OF POULTRY PRODUCTS WHICH WAS OBTAINED USING VITAMIN-MINERAL COMPLEXES WITH SELENIUM IN GROWING TECHNOLOGY

   
Authors: O.I. Sevostyanova1 Candidate of Biological Sciences, V.A. Orobets1 - Doctor of Veterinary Sciences, Professor, A.V. Serov2 - Doctor of Technical Sciences, Associate Professor, E.A. Sokolova1 - candidate of biological sciences, E.S. Kastarnova1 postgraduate student
   
Address:

1Federal State Budgetary Educational Establishment of Higher

 Education Stavropol State Agrarian University,

(355017 Stavropol, Zootechnical per. 12, e-mail: sevostyanova19@mail.ru)

 

2Federal State Autonomous Educational Institution of Higher

Education "North Caucasian Federal University",

(355017 Stavropol, Pushkin street, 1, -mail: sav_ncstu@mail.ru)

   
Abstract:

Aggregate-resistant vitamin-mineral complex has been created, based on selenium with a particle size of 20-60 nm with a complex of lipophilic and hydrophilic vitamins. It is used in the technology of growing broiler chickens, like the Cobb-500 cross. It was studied the effect of thise complex on the chemical composition of poultry meat, including in comparison with an analogue preparation. The ability to classify the resulting product as functional, due to enrichment with selenium, has been established. Group 1 was a control. Group 2 broilers consumed a developed aggregate-resistant vitamin-mineral complex with drinking water. Group 3 chickens used the analog drug Solvimin Selen. The use of vitamin-mineral complexes based on selenium in the diet of broiler chickens not only does not adversely affect the quality of meat products, but also has a definite positive effect: the minced experimental groups had more protein and less fat. A significant increase in dry protein content from dry matter was in the samples of the femoral muscles of group 2 - 9.84%, of group 3 - 12.70%. A significant decrease in fat content is characteristic of both the pectoral and femoral muscles. The cumulation of selenium in the thigh muscles in groups 2 and 3 exceeded the control parameters by 32% and 40%, respectively, in the liver - by 59% and 55%, in the heart - by 40% and 45%. The resulting poultry products can be classified as functional and, in accordance with GOST R 55577-2013, have the additional marking with a high content of selenium.

   
Keywords: poultry, selenium, vitamins, veterinary and sanitary examination, functional products
   
References:

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