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¹ 4, 2019

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Authors: Nizamov R.N. – Doctor of Veterinary Sciences, professor; Konyukhov G.V. – Doctor of Biological Sciences, professor; Sharifullina D.T. – Candidate of Biological Sciences; Titov A.S. – Candidate of Biological Sciences; Shakurov M.M. – Candidate of Biological Sciences.
Federal Center for Toxicological, Radiation and Biological Safety, Kazan, (e-mail: vnivi@mail.ru).

Owing to an increasing interest for the immunostimulating therapy on the background of wide spread of immunodeficiency with signs of insufficiency of the humoral or cellular component of the immune system in animals and efficacy of immune modulating drugs for the stimulation of the natural resistance at this pathology, we conducted research for the purpose of obtaining and evaluation of microbial immunotropic drugs efficacy on radioinduced immunodeficiency of animals. To study this issue, we have obtained the structural components and metabolic products of E. coli, which separately, in different combinations and in the form of a composite monopreparation was used as a stimulant of immunity against the background of radio-induced immunodeficiency. Radiation-induced immunodeficiency was modeled by a single ?-irradiation of white mice in a dose of 7.7 Gy for 7 days before and 7 days after irradiation (on the background of the development of immunological failure) to the animals once subcutaneously injected subcellular, cellular and composite microbial preparations FI, FII, FIII and FIV based microbes E.coli 2.5?109 CFU/kg of endotoxin - 2.5 mg/kg and the products of metabolism - 78 mg/kg. Of the tested microbial components, the most active was the composition FI+FIV, consisting of a mixture of microbial biomass, culture fluid and endotoxin E. coli, which contributed to the increase of natural radioresistance factors of the body, activating the activity of the Central (bone marrow stem cells) and peripheral (thymus, spleen) immune organs.

Keywords: E.coli, acute radiation sickness, metabolic products, Radiation-induced immunodeficiency, microbial substances, bioradioprotector, endotoxin, radioresistance.
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2. Novye podkhody k terapii ostroy luchevoy bolezni [New approaches to therapy of the acute radiation sickness] / G.V.Konyukhov, R.N.Nizamov, N.B.Tarasova, I.N.Nigmatullin // Veterinarny vrach. – 2005. – ¹ 1. – P. 40–43.
3. Radiozashchitnaya aktivnost kompozitsiy na osnove metabolitov E.coli, B.bifidum immunomodulyatorov novogo pokoleniya [Radioprotective activity of compositions based on metabolites E.coli, B.bifidum new aged immunomodulators] / G.V.Konyukhov, R.N.Nizamov, D.T.Sharifullina [et al.] // Veterinarny vrach. – 2016. – ¹ 6. – P. 36–38.
4. Usovershenstvovanie tekhnologii polucheniya radiozashchitnykh preparatov na osnove B.bifidum i E.coli v sochetanii s biopolimerom i otsenka ikh effektivnosti na selskokhozyaystvennykh zhivotnykh [Improvement of production technologies of radioprotective drugs B.bifidum i E.coli combined with biopolymer and evaluation of their effect on farm animals] / G.V.Konyukhov, R.N.Nizamov, D.T.Sharifullina [et al.] // Veterinarny vrach. – 2017. – ¹ 6. –P. 31–35.
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7. Radiozashhitnye kompozitsii na osnove produktov E.coli [Radioprotective compositions based on E.coli products] / R.N.Nizamov, G.V.Konyukhov, À.S.Titov [et al.] // Veterinarny vrach. – 2013. – ¹ 4. – P. 25–27.



Authors: Sarukhanov V.Ya. - Candidate of Biological Sciences; Kobyalko V.O. - Candidate of Biological Sciences.
All-Russian Research Institute of Radiology and Agroecology, Obninsk (e-mail: rirae70@gmail.com).

The purpose of this research was to study the course of radiation sickness as well as the natural resistance with external irradiation of calves. The experimental data obtained in FSBSI “RIRAE” on 5 calves of 6 month age, with an average live weight of 157.0 kg irradiated in a dose of 3Gy, and 3 non-irradiated calves were as control. The animals were irradiated on the GUL-24 unit with a 137Cs source with a dose rate of 2 Gy/h. The bactericidal and ?-lytic activity were determined by the methods of Bukharin’s OM in our modification. The autoflora of the upper respiratory tract was determined by the method of taking the examined material from the mucous membrane of the nasal cavity. To evaluate a clinical condition of the animals as well as counting the leukocytes and thrombocytes concentration common methods were used. The radiation sickness of calves is found to be clinically similar to the radiation sickness of other ruminants of sheep and goats. The death of animals is preceded by an increase in rectal body temperature till 41.20C. Radiation pathology of cattle is accompanied by reduction in leukocytes concentration by 80-90% and thrombocytes – by 30-80% as well as suppression of ?-lytic blood properties by 60% compared to control. The latent period of radiation sickness of cattle lasts for 10-12 days and accompanied only by reduction in leukocytes and thrombocytes concentration as well as suppression of ?-lytic blood activity. The research have shown that the value of the mortality index (IL) obtained on sheep can probably be used to predict the death of irradiated cattle. 

Keywords: emergency situations, cattle, calves, total irradiation, radiation sickness, natural resistance of the body, lethal index.
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6. Sarukhanov, V.Ya. Metod opredeleniya bakteritsidnoy aktivnosti krovi selskokhozyaystvennykh zhivotnykh: metodicheskiye rekomendatsii [Method for the definition of bactericidal activity of agricultural animals blood: methodological recommendations] / V.Ya.Sarukhanov, N.N.Isamov. – M.-Obninsk, 2006. – 8 p. 
7. Metod opredeleniya beta-liticheskoy aktivnosti krovi krupnogo rogatogo skota [Method for the definition of ?-lytic activity of blood of cattle] / V.Ya.Sarukhanov, N.N.Isamov, N.V.Grudina, P.G.Tsarin. – M. – Obninsk, 2006. – 7 p.
8. Mishanin, Yu.F. Prakticheskaya veterinariya [Practical veterinary medicine] / Yu.F.Mishanin, M.Yu Mishanin. – M.: Mart. 2002. – 384 p.
9. Sarukhanov, V.Ya. Otsenka posledstviy vneshnego ?-izlucheniya i ranniy prognoz ostroy luchevoy bolezni u ovets [Evaluation of external ?-irradiation consequences and early forecast of sheep radiation sickness] / V.Ya.Sarukhanov, N.N.Isamov, V.G.Epimakhov // Veterinarnyy vrach. – 2015. – ¹ 1. – P. 40–43.



Authors: 1Mingazov N.D. – Master; 2Umarova N.N. – Candidate of Chemical Sciences; 3Ismailova R.N. – Candidate of Chemical Sciences; 4 Valiev M.M. – Candidate of Agricultural Sciences.
1Veterinary Laboratory of the Republic of Tatarstan, Kazan (e-mail: rvl-rt-kazan@yandex.ru).

2Kazan National Research Technological University, Kazan.

The most important conditions for the success of the testing laboratory is the passage of the accreditation procedure, stability and reproducibility of test results, the absence of complaints from customers and, ultimately, customer satisfaction. Most quality management systems for testing laboratories operate on the basis of the international standard ISO / IEC 17025-2009 General requirements for the competence of testing and calibration laboratories. Since 2017, a new version of this international standard has entered into force - ISO / IEC 17025?2017; In this version, special attention is paid to the risk-oriented approach and management system integration processes. Laboratories in which the integrated management system is already operating will be much easier to adapt to the requirements of the new version of the international standard ISO / IEC 17025-2017 of 2017. Also, when analyzing the literature data, there is a tendency that testing laboratories in which an integrated management system operates have a number of advantages over those testing laboratories in which there is no integrated management system. In order to identify strengths, weaknesses, threats and opportunities, a SWOT analysis of the Testing Center was conducted. Also in this work, an analysis was made of the functioning of the management system of the Testing Center of the Republican Tatarstan Republican Veterinary Laboratory, the product life cycle was analyzed, environmental aspects of the activity and factors that adversely affect the Testing Center staff were identified, the Information Security System of the Testing Center was analyzed. An FMEA analysis of abnormal situations during testing was conducted. The information security system of the Testing Center and its effectiveness were analyzed after the FGIS VetIS - FGIS Vesta components were introduced into the Testing Center. Specific proposals to reduce the negative impact of environmental factors on the staff of the Testing Centerhave been developed. The evaluation of the functioning of the elements of the Management System of the Testing Center was carried out and the rationale for introducing an integrated management system into the activities of the Testing Center as a driving vector for further development was given. Specific proposals for improving the activities of the Testing Center have been developed.

Keywords: testing laboratory, integrated management systems, assessment of the effectiveness of the quality management system of the testing laboratory, environmental aspects, industrial safety and labor protection, information safety of testing laboratories.
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Authors: Papunidi K.Kh. – Doctor of Veterinary Sciences, professor; Biktashev R.U. - Doctor of Agricultural Sciences; Kadikov I.R. – Doctor of Biological Sciences; Zakirova G.Sh. – Candidate of Veterinary Sciences.
Federal Center for Toxicological, Radiation and Biological Safety, Kazan, (e-mail:vnivi@mail.ru).

The paper contains data on productivity of cross Kobb-500 chicken-broilers under background of fodder contamination by 0.5 MPC of cadmium and 0,5 MPC of lead and using shungite and zeolite as separately so in combination in correlation 1:1 in doses of 0.25% and 0.5%. Haematological parameters of blood serum are presented. The dose 0.25% of shungite and zeolite of fodder mass is found to be insufficient and it does not secure the poultry organism protection against cadmium and lead influence. Using of sorbents as separately so combinatively in dose 0.5% secures achievement of productivity of broilers from biological control group and promotes increase of phagocyte activity. Introduction of 0.5% shungite into basic diet (without heavy metals contamination) promotes increase of broilers productivity by 10.2% as compared with biological control. Using of zeolite in the same dose does not give a similar effect.

Keywords: chicken-broilers, diets, cadmium, lead, shungite, zeolite, hematological parameters, phagocytic activity.
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Authors: Ermolaeva O.K. - Candidate of Biological Sciences; Tanaseva S.A. - Candidate of Biological Sciences; Mochtarova S.L. – leading engineer; Semenov E.I. - Candidate of Biological Sciences; Papunidi E.K. - Doctor of Biological Science, professor.
Federal Center for Toxicological, Radiation and Biological Safety, Kazan (e-mail: vnivi@mail.ru).

The article presents the results of veterinary and sanitary examination of poultry meat in mycotoxicoses (T-2 toxicosis, aflatoxins and zearalenones) against the application of shungite and zeolite. The experiments were carried out on broiler chickens of the cross "COBB 500" divided by the principle of analogues into 5 groups (n=5). The first group served as a biological control (BC), received the main diet (RR); the second group received the main diet and a mixture of shungite and zeolite (70:30) at a dose of 0,5% of the diet; the third group received the toxic feed (TC) with mycotoxins in concentrations of T-2 toxin 200mkg/kg, zearalenone 500 µg/kg and aflatoxin B1 50 µg/kg; the fourth group received the toxic feed and the mixture of shungite and zeolite (70:30) at a dose of 0,5% of the diet; the fifth group received the toxic feed and the mixture of shungite and zeolite (70:30) at a dose of 0,25% of the diet. The duration of the experiment was from 21 days of age to the day of the control slaughter, which was performed at the age of 42 days. The meat of the poultry received toxin in the background of using zeolite and shungite When studying the quality and safety of samples of poultry meat treated with the toxin on the background of the application of shungite and zeolite for organoleptic, microbiological and physico-chemical parameters meets the requirements of GOST R 51944-2002, 7702.1-74 and the "Rules of veterinary inspection of slaughtered animals and veterinary-sanitary examination of meat and meat products". With mycotoxicosis of poultry, there is a deterioration of physical and chemical parameters of meat, which are normalized by the addition of enterosorbents to the toxic diet. Normalization of meat indicators is explained by the improvement of resistance of the body and metabolic processes due to the high selective sorption capacity and balanced mineral composition of zeolite and shungite.

Keywords: mycotoxicosis, poultry, shungite, zeolite, poultry meat, veterinary and sanitary examination.

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Authors: 1Papunidi K.Kh. - Doctor of Veterinary Sciences, professor; 1Semenov E.I. - Candidate of Biological Sciences; 1Mishina N.N. - Candidate of Biological Sciences; 2Ugrumov O.V. - Doctor of Engineering Sciences, professor; 3Ugrumova V.S. - Doctor of Veterinary Sciences, professor; 4Yarullin R.S. - Doctor of Chemical Sciences, professor; 5Valiev Ì.Ì. - Candidate of Agricultural Sciences.
1Federal Center for Toxicological, Radiation and Biological Safety, Kazan (e-mail: vnivi@mail.ru).

2 Public Company “Rosneft”, Kazan (e-mail: Olegugr@yandex.ru).

3CEO ZAO SPC “Himtechno”, Kazan (e-mail: vs@himtehno.ru).

4CEO OJSC “Tatneftekhiminvest-Holding”, Kazan (e-mail: jarullin@tnhi.mi.ru).

5Republican Veterinary Laboratory, Kazan (e-mail: Valiev.mars@inbox.ru).

Toxicological and hygienic assessment of the disinfectant Rekobact TU 20.20.14.-031-48680808-2018 was carried out. In accordance with GOST 12.1.007-76, the drug belongs to the 3rd hazard class - substances moderately hazardous. It does not have a pronounced sensitizing effect. The working concentration of the drug (1% aqueous solution) does not irritate the skin. In its native form, Rekobact according to MU 1.2.1105-02 “Assessment of Toxicity and Hazard of Disinfectants” refers to the 3rd class of hazard according to the severity of irritant effect on the skin. Antiseptic for hands "Rekosept" TU 20.20.14.-032-48680808-2018. In accordance with GOST 12.1.007-76, it belongs to the 4th class of hazard - low hazardous substances. It does not have a pronounced sensitizing effect. It does not irritate the skin.

Keywords: toxicological evaluation, disinfectants, antiseptics, cationic surfactants, Rekobact, Rekosept.
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Authors: Mukhammadiev Rin.S. - Candidate of Biological Sciences; Mukhammadiev Rish.S. - Candidate of Biological Sciences; Birulya V.V. - Candidate of Biological Sciences; Skvortsov E.V. - Candidate of Biological Sciences; Idiyatov I.I. - Candidate of Biological Sciences; Valiullin L.R. - Candidate of Biological Sciences.
Federal Center for Toxicological, Radiation and Biological Safety, Kazan (e-mail: vnivi@mail.ru).

Preclinical study involving new drugs based on biologically active substances produced by using biotechnological methods are determined by the requirements for existing drugs obtained by the new technology and should be based on safety studies on adequate in vivo and in vitro models. A study was made concerning the cytotoxicity in vitro of lactic and propionic acid bacteria against embryonic cow embryo lung epithelial cell lines (LEK), human skin fibroblast (HSF), and human embryo kidney (HEK-293). Strains Lactobacillus salivarus, Lactobacillus plantarum, Enterococcus faecium, Lactococcus lactis and Propionibacterium freudenreichii were used as lactic acid and propionic acid bacteria. The cytotoxic effect of lactic and propionic acid bacteria was determined by survival of cell cultures using the standard MTT assay and staining cells with trypan blue. The combined incubation of lactic and propionic acid bacteria at concentrations of total protein of 0,25-1050 µg/ml with against HEK-293 human embryonic kidney, HSF human skin fibroblast and LEK cow embryo lung epithelial cell lines did not lead to suppression of cell growth in culture. The morphology of cell cultures after their combined incubation with lactic and propionic acid bacteria was not significantly different from the morphology of control cells. The data obtained by MTT assay and staining cells with trypan blue are confirmed by the results of cell morphology, obtained by light microscopy and indicate the safety of strains of lactic acid and propionic acid bacteria and the possibility of using these microorganisms as probiotics in veterinary medicine.

Keywords: probiotics, lactic acid, propionic acid bacteria, cell culture, morphology, survival, cytotoxicity.
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Authors: Spiridonov G.N. – Doctor of Biological Sciences; Dupleva L.Sh. - Candidate of Biological Sciences; Khusainov I.T. – junior researcher; Saripov A.S. - Candidate of Biological Sciences; Makaev Kh.N. – Doctor of Veterinary Sciences, professor.
Federal Center for Toxicological, Radiation and Biological Safety, Kazan (å-mail: vnivi@mail.ru).

The article presents the research data concerning development of the associated vaccine against infectious keratoconjunctivitis of cattle based on antigens of bacteria Moraxella bovis and Moraxella bovoculi. The patented strains Moraxella bovis “G97-VNIVI” and Moraxella bovoculi “SKh-Ch ¹6-DEP” isolated from keratoconjunctivitis calves were used as production ones. 3 experimental series of inactivated vaccine have been made. Each series was tested for sterility, harmlessness and antigenic activity. The vaccine was found to be sterile, harmless for laboratory animals and for cattle having a pronounced antigenic and immunogenic activity. Double subcutaneous introduction of vaccine to rabbits in a dose of 2 cm3 causes accumulation specific antibodies in blood serum to Moraxella bovis and Moraxella bovoculi in titers 1:3733,33±1728,20 – 1:7466,67±3456,40. Production testing of the experimental series of vaccine was conducted in 6 livestock farms of the Republic of Bashkortostan and the Republic of Tatarstan to be infectious for keratoconjunctivitis of cattle. The disease rate of calves under 6 months age before using vaccine was 30-56%, young cattle under 12 months age – 27-36%, and young cattle after 1 year age and also adult livestock – till 8%. The vaccine was used double with preventive purpose before sending cattle to pasture with an interval of 21-30 days in next doses: calves under 6 months age – 3 cm3, young cattle from 6 till 12 months – 4 cm3, young cattle after 1 year and adult livestock – 5 cm3. The vaccine was found to have a pronounced immunogenic activity. Preventive use of the vaccine made it possible during the year to completely recover 4 farms from infectious keratoconjunctivitis, and in the other 2 farms – to reduce the disease till 2-3%. The vaccine is patented in the Russian Federation, the standard documents have been developed. The positive results of laboratory and production testing of the vaccine make it possible to recommend it to be implemented into the veterinary practice of the Russian Federation.

Keywords: infectious keratoconjunctivitis, cattle, Moraxella bovis, Moraxella bovoculi, specific prevention
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Authors: Evdokimov N.V. - Doctor of Agricultural Sciences, professor; Nemtseva E.Yu. - Candidate of Agricultural Sciences.
Chuvash State Agricultural Academy, Cheboksary (e-mail: EUNemtzeva@yandex.ru).

The article presents the results of the comparative characteristics of milk production of cows and bulls obtained by transplantation in the Chuvash Republic. 13 heifers and 3 bulls were obtained by embryo transplantation method, the engraftment of embryos amounted to 61.5%. Comparing the growth parameters of 18 months calves the bulls by this age were revealed to excel their peers by 8.0 kg for living weight, and the heifers – by 12.0 kg. The results of the measurements taken showed that the transplants had a difference over peers in height at the withers 1 cm, oblique body length 3 cm, the chest girth by 3 cm, width of chest, macluckie and hip joints by 1 cm, but inferior to the metacarpus 1 cm. Milk yield of heifers-trasplants and their peers during the lactation period amounted to 6948 and 6352 kg, respectively, with an equal percentage of fat content 3.7 % (difference of 14.5% in favour of the first ones). Productivity comparison of the first-calf transplants with the productivity of recipient cows showed a difference in favor of transplants by 9.4 % or 596 kg. Producer bulls were evaluated by the quality of offspring by two methods: the method of the daughter-peer and the method of the producer index. Found that of proven bulls after improver for the yield of milk and geronimoleft The servicing bull named Globus 0054 is found to be the improver for milk yield and for milk fat content from the evaluated bulls, and as for only the yield of milk the improvers are two of the bulls–brothers – Globus 0054 and Grad 0052.

Keywords: embryon, servicing bulls, donor cows, recipient cows, milk productivity.
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Authors: 1Azhmuldinov E.A. – Doctor of Agricultural Sciences, professor; 1Kizaev M.A. – Candidate of Agricultural Sciences; 1Titov M.G. – Candidate of Agricultural Sciences; 2Babicheva I.A. – Doctor of Biological Sciences.
1Federal Research Centre of Biological Systems and Agro-technologies of the Russian Academy of Sciences, Orenburg (e-mail: Titow.ru@mail.ru).

2Orenburg State Agrarian University, Orenburg (e-mail: rector@orensau.ru).

The article presents information on the comparative analysis of beef quality and the characteristics of bioconversion of feed nutrients into meat products in bull-calves with different genetic potential. Four groups were formed to study the biological potential of individuals: I - Simmental breed, II – Black-and-White, III – Red Steppe and IV – Kalmyk. During the research, the experimental animals were kept according to the technology adopted at the feedlot of Joint Stock Company named N.E.Tokarlikova in Tatarstan Republic on rations designed to obtain 900-1000 g of average daily gain. The highest rates of growth intensity were observed in Simmental bull-calves when analyzing the growth traits. They exceeded contemporaries from other studied groups by average daily gain by 4.0-9.9%. Individuals of this breed also occupied an advantageous position in terms of meat productivity parameters, in particular, by hot carcass weight - by 3.5–9.5%, flesh - by 3.8–12.0%. At the same time, bulls for the meat direction of productivity had more favorable position in slaughter products yield and its qualitative composition. They had higher hot carcass yield by 0.7-1.9%, flesh yield – by 0.7-2.4% and meat index – by 4.2-12.9% compared with animals from I, II and III group. Biological features of studied animals had a significant effect on nutrient synthesis in meat products. Thus, Simmental bull-calves were ahead of analogues from other groups by the level of protein in the edible part of the body by 2.6–9.5%, and Kalmyk animals had the predominant position in fat deposition by 16.5-40.8%. The same trend was established in its yield per 1 kg of the pre-slaughter weight, as well as bioconversion of protein and feed energy in meat products. With a deeper assessment, relatively high indicators in biological value of muscle tissue were observed in calves of Kalmyk breed. Their level of protein quality value was higher by 2.2-10.7% compared with the contemporaries from other groups.

Keywords: bull-calves, meat productivity, nutrient yield, bioconversion, biological value.
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Authors: Alekhin Yu.N. - Doctor of Veterinary Sciences; Morgunova V.I. - Candidate of Veterinary Sciences; Kashirina L.N. - Research Assistant.
All-Russian Veterinary Research Institute of Pathology, Pharmacology and Therapy, Voronezh (e-mail: exterapi@yandex.ru).

Under the conditions of a dairy farm, using standard clinical, instrumental and laboratory methods, studies were conducted to study the characteristics of the biochemical status of calves (n = 299) with different body weight at birth. It has been shown that Holstein breed calves born with a body weight of 36.5 to 29 kg do not have significant differences in protein, carbohydrate and lipid metabolism. Therefore, this weight range corresponds to the normal level of their intrauterine development. In infants with less weight, metabolic disorders were detected, which gives grounds for finding out of their antenatal hypotrophy. The decrease in body weight at birth by 1 kg causes the activation of protein and carbohydrate metabolism. At that accumulation of lactic acid and a decrease in the activity of aminotransaminases (AST by 29.8%, and ALT by 33.4%), indicating a violation of the mechanisms regulating these exchanges. A weight deficit of more than 2 kg caused an intensification of lipid metabolism. With a decrease in body weight of less than 27 kg, hypoglycemia, lactic acidosis and hypoproteinemia are observed, and a decrease in weight to 25 kg caused the development of endogenous intoxication. Thus, in newborns weighing less than 29 kg, significant metabolic disorders were detected, the severity of manifestation of which depends on the degree of weight deficit. At first, it is the activation of metabolic processes, the mobilization of reserves of energy substrates and, first of all, lipids. With the increased severity of hypotrophy, the catabolic orientation of the metabolism and the severity of its failure with the accumulation of toxic products, leading to the development of lactic acidosis, endogenous intoxication and liver pathology, increase.

Keywords: cattle, newborn calves, hypotrophy, protein metabolism, carbohydrate metabolism, lipid metabolism, endogenous intoxication
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Authors: 1Musaev A.M. - Research Assistant; 2Aliev A.A. - Doctor of Biological Sciences.
1Dagestan Agrarian Scientific Center of the Republic of Dagestan - a branch of the “Caspian Zonal Research Veterinary Institute”, Makhachkala (e-mail: Gamid-utamish@mail.ru).

2Dagestan State Agrarian University named after M.M.Dzhambulatov, Makhachkala (e-mail: daggau@list.ru).

Currently, there are lots of domestic and imported detergents and disinfectants offered by science and practice to sanitate veterinary facilities. At the same time, among them there are very few available, effective and harmless disinfectants and insectoacaricides. Among such means having both disinfecting and insectoacaricidal properties the disinfecting compounds based on neutral anolyte can be related. The purpose of the research is to study the disinfecting efficiency of harmless compounds based on neutral anolyte used to disinfect poultry facilities surfaces. The experiments were carried out at the Krasnoarmeysk poultry farm of the Republic of Dagestan in poultry facilities of 100×12×4.5 m sized with floored keeping of broiler chickens in the amount of 18 thousands heads at the age of 25 days. To test the disinfecting efficiency of the tested compound, the next test-objects were used: mechanically cleaned surfaces of the floor, walls, and feeders. Directing aerosols of the tested compounds were applied to these objects using automatic nebulizer Orion with an average median particle diameter of an aerosol of 150–200 ?m and a flow rate of 0.2-0.4 l/m2. Evaluation of the disinfecting efficiency of the tested compound was performed before and after the disinfection in 30 and 60 minutes of exposure. The disinfecting effectiveness of a neutral anolyte with a concentration of active chlorine of 0.2 mg/ml in combination with 0.1% salicylic turpentine suspension of the poultry rooms surfaces was at exposures of 30 and 60 minutes, respectively, the floor – 89.1-92.8%, the wall – 75.74-86.70%. Therefore, the harmless disinfecting compound based on a neutral anolyte is an effective disinfectant that can be recommended to be introduced into poultry farms of the Republic of Dagestan.

Keywords: neutral anolyte, broiler chickens, disinfection, exposure, CFU, E. coli, St. aureus, beef-extract agar.
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Authors: Ulitko V.E. - Doctor of Agricultural Sciences, professor; Desyatov O.A. - Candidate of Agricultural Sciences; Chernyshkova E.V. - postgraduate; Pykhtinà L.A. - Doctor of Agricultural Sciences, professor; Kornienko A.V. - Doctor of Agricultural Sciences; Lomakin A.A. - Master.
Ulyanovsk State Agrarian University named after P.A. Stolypin, Ulyanovsk (e-mail: kormlen@yandex.ru).

Experimental studies conducted on three groups of calves of the black-and-white breed found that the use of a sorption and probiotic additive Biopinular in the amount of 0.5 and 1.0% of the dietary dry matter in the diet during the milk period optimized the microbiocenosis in the rumen and the large intestine in the direction of a sharp increase in the development of Lacto- and Bifido bacteria and inhibition of the development of pathogenic microorganisms. Along with this, the studied additive contributes to a deeper fermentation of the forage mass in the process of cicatricial digestion, manifesting in an increase in the active acidity (pH), cellulolytic activity of bacteria, the content of VFA as the final product of fermentation of carbohydrates, as well as enhancement of the ammonia-binding microflora function, which determined the best energy and protein provision of their body and a significant increase in the intensity of increase in live weight, as evidenced by a large average daily (respectively 8.8 3 and 23.70%) and relative (by 1.63 and 3.86%) increment and lower costs (0.44 and 0.64) of EFU per unit of increment.

Keywords: sorption-probiotic additive Biopinular, calves, digestive tract microbiocenosis, cicatricial digestion, productivity, feed conversion.
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