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น 4, 2018



Authors: 1ArisovM.V. – Doctor of Veterinary Sciences; 2Belykh I.P. – Candidate of Veterinary Sciences; 1Artemov V.V. – postgraduate.
1All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K.I.Skryabin (e-mail: arisov_vet@mail.ru).

2JSC "Research and Production Company "Ekoprom" (e-mail: belykh@ekoprom.org).

The article presents the results of studying the allergenic properties of the new combined drug for veterinary use "Inspector Quadro" (praziquantel, fipronil, pyriproxifen, moxidectin) intended for the treatment and prevention of arachnoentomosis, nematodosis, cestodosis in dogs and cats. For the study, 50 guinea pigs weighing 300-320 g were taken. Epicutaneous sensitization was carried out on 40 guinea pigs by 20 repeated (5 times per week) cutaneous applications on the sides of the trunk without wool 2 x 2 cm in a dose of 0.05 ml (1 drop) solution, applying a uniform layer on the entire site. Ten days after sensitization, the cutaneous (epicutaneous), intradermal, conjunctival and nasal samples were performed; serum from sensitized and control animals was tested in an indirect mast cell degranulation (NTDT) reaction. 10 sensitized guinea pigs and 10 non-sensitized controls were used for each sample (saline was applied). When assessing the epicutaneous test, it was established that within 72 hours after the application of the preparation to the sensitized and control animals, the treated areas did not differ in the pattern of the skin reaction (absence of hyperemia, infiltration) in the experimental and control guinea pigs. When the intradermal test was performed after 48 hours, slight peeling was observed on the skin of the sensitized and control guinea pigs. Fixed deviations during 12 hours of observation, with conjunctival and nasal samples in both groups, indicate a mild effect of the drug on the mucous membranes. The indicators obtained during the reaction of indirect degranulation of mast cells did not differ in the groups of sensitized and control animals, which also indicated the absence of allergic properties in the preparation. On the basis of the obtained test results the preparation with a 20-fold cutaneous sensitization in a dose of 0.05 ml is not found to cause allergenic activity of animal’s organism.

Keywords: drug, solution, praziquantel, fipronil, pyriproxifen, moxidectin, sensitization, degranulation, guinea pigs.
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Authors: 1Mustafina E.N. – Candidate of Veterinary Sciences; 2Mustafin T.R. – Candidate of Biological Sciences; 1Sadykov N.S. – Doctor of Veterinary Sciences, professor.
1Federal Center for Toxicology, Radiation and Biological Safety, Kazan (e-mail: vnivi@mail.ru).

2The company "PromAseptika", Kazan (e-mail: swa862@mail.ru).

One of the main tasks for developing new ways of diagnosing and treating botulism is to study its toxins. For this reason, in this study we compare and evaluate two methods of obtaining and accumulating a native neurotoxic complex (toxin) Cl. Botulinum type A. The first method of obtaining and accumulating a neurotoxic complex is the toxin concentration by an inorganic binary compound, an ammonium salt of sulfuric acid. The second method follows the way of salting out the native neurotoxic complex (toxin) Cl. Botulinum type A, the same ammonium sulfate, but under other conditions, the amount and method of introducing it into the culture liquid. Along with the foregoing, the methods differ in the preparation of the culture fluid obtained from Clostridium botulinum type A. Based on the results of experiments comparing methods of production and accumulation of the native neurotoxic complex (toxin) Cl. Botulinum type A, the most effective and specific, is the method of concentration of toxin by ammonium sulfate.

Keywords: botulism, toxin, ammonium sulfate, concentration, salting out.
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Authors: Tukhfatulov M.Z. – applicant.
Federal Center for Toxicological, Radiation and Biological Safety, Kazan (e-mail: vnivi@mail.ru).

The model of the experimental radiation stress (RS) was applied on 40 white rats divided into four groups: 1 group – experimental animals; 2 group – animals exposed to radiation; 3 group – irradiated and taken stress protector apiphytopreparation “Vita-Force M” followed by exposure. The modeling of radiation stress was carried out by once external exposure of animals at a dose of 7.7 g on the gamma-installation “Puma” with radiation source of Co 60. Every day, the animals was taken 0.1 g/kg apiphytopreparation “Vita-Force M” with feed for 10 days before exposure (3 group) and for 10 days after exposure (4 group). To evaluate antioxidant efficacy of the preparation we studied degree of malonic dialdehyde accumulation as well as activity of one of the main enzymes of antioxidant protection - peroxidase. The radiogenic stress was found to induce the intensification of lipid peroxidation processes, increasing the concentration of malondialdehyde (MDA) in the blood serum by 39.8% compared to the control while inhibiting the activity of peroxidase enzyme. The use of apiphytopreparation on the background of radiogenic stress was accompanied by a favorable course of acute radiation sickness (survival rate of 66% with 100% death of lethally irradiated animals), at which peroxidase activity and MDA concentration were close to those of the control (nonirradiated) group.
Keywords: radiogenic stress, gamma-exposure, enzyme of antioxidant protection – peroxidase, the product of peroxidation malondialdehyde, apiphytopreparation “Vita-Force M”.
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Authors: 1,2Shuralev E.A. – Candidate of Veterinary Sciences; 1Faizov T.Kh. – Doctor of Veterinary Sciences, Professor; 1Khammadov N.I. – Candidate of Biological Sciences; 1Osyanin K.A. – Candidate of Biological Sciences; 1Usoltsev K.V. – Candidate of Veterinary Sciences; 1,2Aleksandrova N.M. – Candidate of Biological Sciences.
1Federal Center for Toxicological, Radiation and Biological Safety, Kazan (e-mail: vnivi@mail.ru).

2Kazan Federal University, Kazan (e-mail: public.mail@kpfu.ru).

The evaluation of the antigenic activity of the engineered synthetic polypeptide of the immunogenic epitope MPB70 for the detection of specific antibodies in cattle tuberculosis (CRS) is considered. Using the bioinformatic analysis, the antigenic determinant of the MPB70 protein, the most specific for the virulent strains of Mycobacterium bovis, was determined. At the next step, a polypeptide pMPB70m was designed and synthesized which was used as an antigen in an enzyme-linked immunosorbent assay (ELISA). The readings of the IFA response with serum samples from 54 animals from healthy farms were 0.050 ... 0.350 OD, which was interpreted as a negative. When 47 samples of blood serum from the farm with persisting tuberculosis were tested by ELISA, the antibodies to pMPB70m were detected at a high level (0.500 ... 3.500 OD) in 19 animals and were interpreted as positive, in 6 animals the results were at a low level (0.360 ... 0.470 OD) and interpreted as. Inconclusive. In 22 animals, the specific antibodies were not detected, and the ELISA results were in the range of 0.050 ... 0.350 OD, which were interpreted as negative. The ELISA mean values of the optical density in the groups were: 0.175ฑ0.102 OD for negative, 0.415ฑ0.044 OD for inconclusive, and 1.199ฑ0.788 OD for positive samples. The interpretation of the ELISA results with the antigen pMPB70m was statistically confirmed to be reliable (p<0.05) when using the criteria: ≤0.350 OD – the result was negative; 0.350 ... 0,500 OD – the result was inconclusive; ≥0.500 OD – the result was positive. Thus, the high antigenic activity of pMPB70m for the detection of specific antibodies in cattle with tuberculosis was confirmed.

Keywords: Mycobacterium bovis, tuberculosis, cattle, antigens, antibodies, serological diagnosis.
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Authors: 1Efimova M.A. – Doctor of Biological Sciences; 1Mukhamedzhanova A.G. – postgraduate; 1Chernov A.N. – Doctor of Biological Sciences; 1,2Khaertynov K.S. – Candidate of Biological Sciences; 1Akhmadeev R.M. – Candidate of Veterinary Sciences; 1Nasyrov Sh.M. – Candidate of Veterinary Sciences; 1Samerkhanov I.I. – Researcher Officer; 1Aleeva Z.Z. – Researcher Assistant; 1Arutyunyan G.S. – Researcher Assistant; 1Yarullina G.M. – Researcher Assistant.
1Federal Center for Toxicological, Radiation and Biological Safety, Kazan (e-mail: antonina-95@yandex.ru).
2Kazan State Medical Academy – Branch Campus of Russian Medical Academy of Continuous Professional Education, Kazan.

To isolate and purify antirabic immunoglobulins we used serum of sheep immunized with a highly purified rabies virus antigen from the strain “Ovechiy” of the GNKI. The globulin isolating was carried out by the method of triple reprecipitation with a saturated solution of ammonium sulfate, with subsequent dialysis in the buffer system, which resulted in the preparation of purified fractions. For the preliminary evaluation of these fractions activity we used electrophoresis in a 7.5% separating polyacrylamide gel. This characteristic of the investigated fractions was confirmed by the method of indirect immunoenzyme (ELISA) with homologous and heterologous antigens of the rabies virus. Subsequently, the obtained immunoglobulins were labelled with fluorescein-5-isothiocyanate (FITC) followed by gel filtration using gel Sefadex G-250. The activity and specificity of fluorescent immunoglobulins were assessed by the method of fluorescent antibodies (MFA) in brain prints of mice infected with rabies virus and Aujeszky's disease virus, in brain prints of intact mice as well as according to indicators of concentration of labelled protein and the molar ratio of FITC / protein. We identified the most active immunoglobulin fractions and determined their optimal working dilutions, that in prospects will make it possible their using as specific components of express test systems to diagnosing rabies based on ELISA and MFA.

Keywords: rabies virus, antirabic immunoglobulin, electrophoresis, immunoenzyme analysis, immunofluorescence.
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Authors: Kosarev M.A. – Candidate of Biological Sciences; Fomin A.M. – Doctor of Veterinary Sciences, professor; Safina G.M. – Candidate of Veterinary Science; Grigorieva S.A. – Research Assistant; Tuhvatullina L.A. – Research Assistant.
Federal Center for Toxicological, Radiation and Biological Safety, Kazan (e-mail: vnivi@mail.ru).

The purpose of the research was to study the cultural and morphological properties of Brucella in different degrees of dissociation. Cultural and morphological properties of strain of Brucella B. abortus R-1096 were studied in comparison with control strains B. abortus 19 (S-form) and 82 (SR-form) in the set of tests: speed and character of growth on nutrient elective media – liver-peptone glucose-glycerol agar (PPGA), growth on nutrient media with dyes (thionin 1:25 million, 1:50k; 1:100k; 1:50k; 1:100 thousand), hydrogen sulfide ability, the need for an increased content of carbon dioxide, color of the colonies at white-Wilson, sensitivity to penicillin (0.5; 5; 50; 100 U/ml), antigenic properties of cultures using specific Brucella S- and R-serums and minor "A" and "M" of the serum, the sample of thermoagglutination, test with trypaflavine (acriflavine). The culture of the strain R-1096 in all terms of thermoagglutination registring as well as in the sample with tripaflavin showed a positive results. The most intense release of hydrogen sulfide was registered in R-1096 in comparison with other vaccine strains of B. abortus – 19 and 82. Strain R-1096 showed the growth in all dilutions of aniline dyes and also showed continuous growth in media where the concentration of drug was 0.5 – 5.0 and 50 U/ml. The study of cultural and morphological properties of Brucella in different degrees of dissociation showed the distinctive properties of the culture of the R-1096 strain in comparison with other studied strains in all tests.

Keywords: brucellosis, dissociated form, cultural and morphological properties.
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11. Lyamkin, G. I. Epidemiologicheskaj obstanovka po brucellesu v Yuzhnom federalnom okruge [The Epidemiological situation for brucellosis in the Southern Federal district] / G.I.Lyamkin // Problemj sovremrnnoj epidemiology. Perspektivnie sredstva i metodi laboratornoi diagnostiki i profilaktiki aktualnih infectij: Tr. Vseros nauch. conf.; 19–20 Noyabr. 2009. – SPb, 2009. – P. 242. [The problems of modern epidemiology. Prospective means and methods for laboratory diagnostics and prevention of actual infections: proceedings of All-Russian conf., November 2009. – SPb, 2009. – P. 242.]
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Authors: 1Afonyushkin V.N. – Candidate of Biological Sciences; 1Donchenko N.A. – Doctor of Veterinary Sciences; 1Bushmeleva P.V. – Candidate of Veterinary Sciences; 2Barsukova M.A. – Candidate of Biological Sciences; 3Frolova ฮ.ภ. – Research Assistant.
1Siberian Federal Scientific Centre of Agro-BioTechnologies of the Russian Academy of Sciences, Krasnoobsk (e-mail: lisocim@mail.ru).

2Federal State Budget Educational Institution of Higher Education Novosibirsk State Agrarian University, Novosibirsk (e-mail: rector@nsau.edu.ru).

3Krasnoyarsk Research Institute of Animal Husbandry – Separate Division of the Federal Research Center “Krasnoyarsk Science Center” of the Siberian Branch of the Russian Academy of Sciences (e-mail: kristal_o@bk.ru).

The high intensity of production, the high concentration of animals and poultry per unit area, the large size of agricultural enterprises in the Russian Federation – all have a significant impact not only on the growth of animal infection but also create the prerequisites for the emergence of new infections, including potentially dangerous for humans. Associative genetic studies are needed to find markers of resistance to infection by vertically transmitted infectious agents or to more carefully select breeding animals that are not infected with actual infectious agents (pathogens of mycoplasmosis and salmonellosis in birds, pathogens of the reproductive and respiratory syndrome, swine fever in pigs). Modern methods for assessing genetic diversity should be used more widely, including when controlling the movement of livestock products from epizootic unsuccessful farms and vaccine preparations (for the source of origin of the appropriate batch of vaccine). The molecular genetics control of the breeding centers of birds and pigs both domestic and supplied from abroad is of great importance. Identifying the genetic heterogeneity of the party of parental forms makes us doubt that these animals really came from the corresponding tribal center, and are not rejected individuals from an epizootically unsuccessful enterprise. Molecular genetics methods for estimating the genetic diversity of agricultural animals are of more utilitarian interest. For example, the identification of counterfeit vaccines by identifying cell culture lines or lots of developing chick embryos on which vaccine strains of viruses were cultivated plays an important role in ensuring the country's infectious safety. It seems promising to adapt the SNP analysis to the minimum park of equipment available in veterinary and genetic laboratories and institutions, as well as the development of point-of-care testing for the most popular tests. The latter direction is developing especially quickly and is closely connected with informatization and application of various kinds of household mobile devices.

Keywords: selection, broiler chicken, swine, productivity, resistance to disease, PCR, DNA.
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Authors: Kononovich N.A. – Candidate of Veterinary Sciences; Popkov A.V. – Doctor of Medical Sciences, professor; Gorbach E.N. – Candidate of Biological Sciences; Stogov M.V. – Doctor of Biological Sciences; Popkov D.A. – Doctor of Medical Sciences, professor of the Russian Academy of Sciences.
Russian Ilizarov Center “Restorative Traumatology and Orthopaedics” of the RF Ministry of Health, Kurgan (e-mail: office@rncvto.ru).

The implant surface should have osteoinductive properties in order to provide the best osteointegration and, accordingly, the strength of implant-bone fixation. Calcium-phosphate compounds resemble bone tissue chemical composition best of all. There are various techniques of applying such compounds to implants. The specific characteristics of the kinetics of calcium and phosphate releasing from the bioactive layer applied to a metallic titanium-containing substrate were studied in experiments in vitro. Samples were incubated in electrolyte solution for 28 days. In Group 1 the layer was applied by microarc oxidizing method (MAO), in Group 2 – by the method of high-frequency magnetron spraying (HFMS). At the end of the experiment the calcium-phosphate layer condition was studied visually and by scanning electron microscopy technique. The higher adhesion strength of the bioactive layer to the metal substrate was determined in MAO. In Group 1 the ัเ content was 3.1-fold reduced; ะ content – 1.13-fold reduced. In Group 2 the ัเ was 6.54-fold decreased; ะ – 2.38-fold decreased. The study results had demonstrated that the mechanisms of osteoinductive action of the calcium-phosphate spraying might be related to the Ca diffusion from the implant surface into surrounding tissues. The degree of activity of these processes may depend on the technique of bioactive coating applying. In case of HFMS using the stimulating action is more short-term comparing with MAO method. These data should be taken into consideration when treating injuries and other pathological conditions of bones with using products coated with bioactive calcium-phosphate spraying. 

Keywords: experiment, bone, implant, bioactive coating, diffusion, calcium, phosphorus.
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5. Kalciyfosfatnye pokrytiya, sozdannye metodom vch-magnetronnogo raspyleniya gidroksiapatita: osteogenny potentsial in vitro i in vivo [Calcium phosphate coatings created by the HF magnetron sputtering method of hydroxyapatite: osteogenic potential] / M.A.Surmeneva, R.A.Surmenev, I.A.Xlusov [et al.] // Izvestiya Tomskogo politekhnicheskogo. universiteta. – 2010. – Vol. 317(2). – P. 101–106. 
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Authors: Zhukov M.S. – Candidate of Veterinary Sciences; Alekhin Yu.N. – Doctor of Veterinary Science; Nikulina I.R. – postgraduate.
State Scientific Institution All-Russian Veterinary Research Institute of Pathology, Pharmacology and Therapy of the Russian Academy of Agricultural Sciences, Voronezh (e-mail: maxim.zhukoff2015@yandex.ru).

The paper presents the results of a study on the effect of ketoprofen and flunexin miglumine on the hemostasis system of calves with acute respiratory diseases. The study involved 4-5 month old animals (n=24) diagnosed with acute catarrhal pneumonia and clinically healthy. From the number of these calves, according to the principle of selection of analogues, 4 groups of 6 heads each were formed. Group 1 (n=6, positive control) included clinically healthy animals that did not receive pharmacological drugs, in No.2, No.3 and No.4 – animals with bronchopneumonia receiving a course of antimicrobial drugs. In addition, calves in group 3 were injected with intramuscular injection of ketoprofen, at a dose of 3 mg / kg intramuscularly, and No. 4 (n=6) - flunixin meglumine, at a dose of 3.69 mg/kg. The data obtained showed that the use of NSAIDs in bronchopneumonia in calves had antipyretic effect, which was more pronounced after the administration of flunixin meglumine. The koprofen and flunixin meglumine was also found to have an effect on the hemostasis system of calves with bronchopneumonia. The beginning of this influence begins after 2 hours, but clinically significant changes are observed after 4 hours. However, the most pronounced and long-lasting effect was rendered by flunixin meglumine.

Keywords: calves, ketoprofen, flunixin meglumine, hemostasis, bronchopneumonia.
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Authors: 1Schitkovskaya T.R. – Candidate of Biological Sciences; 1D.G.Latypov – Doctor of Veterinary Sciences; 2Gaysina L.A. – Candidate of Veterinary Sciences.
1Bauman N.E. Kazan State Academy of Veterinary Medicine, Kazan (e-mail: gtr110285@rambler.ru).

2Kazan Cooperative Institute, Kazan (e-mail: ya40360@mail.ru).

The aim of this study was to test various helminthoscopic methods in the diagnostics of associative horse infestations. The improved Darling method with the use of trichingredient flotation fluid was the most effective for horses' nematodes. This method has an optimal combination of various factors: density (ρ-1.460), viscosity (η-2.86), transparency of the surface film, slow crystallization of droplets on the glass, which contributes to increased diagnostic efficiency. We tested flotation solutions from these same ingredients and with a higher density of p-1.480-1.520, but with increased density, the viscosity increases and the crystallization time of droplets on the glass also increases, and the surface film becomes contaminated with undigested particles of the feed, making it difficult to detect helmintThus, the improved Darling method with trichingredient flotation mixture ZnCl2, NH4NO3, C3H8O3, where the density of the flotation solution is 1.460, and the viscosity of η-2.86, makes it possible to diagnose causative agents of associative infestations of horses, even at low invasion rates. 

Keywords: diagnostics, horses, nematodes, parascarosis, oxyurosis, intestinal strongylatoses.
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Authors: Raickaya V.I. – Candidate of Veterinary Sciences.
Institute of Agrarian Problems of Khakassia, Abakan (ๅ-mail:raickaya19@mail.ru).

The article analyzes the changes of some hematological and biochemical parameters of blood of different age and sex groups cattle of Hereford breed in different seasons of the year. The spring blood parameters differ significantly from autumn and summer ones, while in the same seasons these parameters are very close to each other. Reducing the number of red blood cells in the blood of animals of different age groups may be caused by inadequate feeding in winter due to lack of proteins. In winter, the content of red blood cells in the blood of bulls was 3.7 g/l, of cows – 4.1 g/l, heifers 4.4 g / l, which were below than the physiological norm by 41.27%, 34.92%, 30.16%, respectively, and in the spring, summer and autumn seasons - stabilized. In winter, calcium decreases in all age and sex groups: in manufacturing bulls - up to 6.6 mg/%, bulls (weaning) – up to 6.1 mg/%, cows – up to 7.8 mg/%, heifers – up to 6.6 mg/%, which are below the physiological norm, respectively – by 30.32%, 36.00%, 16.95 and – 30.74%, and in the spring, summer and autumn periods  - corresponds the established norm. There are slight changes in cholesterol and alkaline phosphatase in animals blood serum, which may be caused by lack of individual minerals in feed (zinc, magnesium) and vitamins. Therefore, in winter and spring it is necessary to carry out preventive measures aimed at the normalization of carbohydrate, mineral, vitamin and protein metabolism.

Keywords: Hereford breed, hematological and biochemical parameters of blood.
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Authors: Grudina N.V. – Doctor of Biological Sciences; Bydanova V.V. – Candidate of Chemical Sciences; Grudin N.S. – Candidate of Veterinary Sciences.
All-Russian Research Institute of Agricultural Radiology and Agroecology, Obninsk (e-mail: n.grudina2014@yandex.ru).

One of the ways of solving the problem of improving meat and milk productivity of farm animals is the use of different methods and means reducing the degree of decomposition of the protein in high concentrated protein feeds of the rumen. Each of the existing methods for reducing the protein disintegration in the rumen is effective in its own way. But these means of "protecting" the protein have significant drawbacks, which limit their application. Therefore, an important and promising task is development of new drugs for the effective "protection" concentrated protein feeds from microbial decay in the rumen of ruminant animals.  This article presents the results of the study, indicating the possibility of using high molecular weight water-soluble polymers (HWP) to reduce decay of protein in the rumen. HWP had a linear molecular structure and a molecular mass of 1x107 Da. The polymer was introduced into the diet with concentrated food. The experiment also shows that the effect of HWP is accompanied by an optimization of the habitat of the bacterial flora of the rumen, which contributes to the normalization of metabolic processes in ruminant animals, especially in case of the concentrating type of feeding cattle. 

Keywords: ruminant animals, rumen, protein, "protecting" the protein, high molecular weight water-soluble polymers.
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Authors: Krupin E.O. – Candidate of Veterinary Sciences; Shakirov Sh.K. – Doctor of Agricultural Sciences, professor; Tagirov M.Sh. – Doctor of Agricultural Sciences, academic Academician of the Academy of Sciences of the RT
Tatar Research Institute of Agriculture – Subdivision of the Federal Research Center ซKazan Scientific Center of Russian Academy of Sciencesป, Kazan (e-mail: tatniva@mail.ru).

The results of a hematological study of blood, as well as the results of biochemical analysis of blood serum (protein metabolism parameters) in dry and fresh cows are presented. In studies, the effect of the use of various vitamin-mineral premixes (P60-3/2 and P60-3/P) in the diets of animal feeding in the complex with 1.2-propanediol and without it evaluated. The article is prepared within the framework of the state task of FASO Russia project ภภภภ-ภ18-118031390148-1. The methods of morphological investigation of blood, methods of biochemical study of protein metabolism parameters of blood serum, mathematical and statistical methods were used in the work. Studies have established that the use of various feeds in the rations of feeding animals and in different periods of time has an effect on the morphological and biochemical composition of blood and serum, respectively. Changes are established two weeks before calving and on the third day after calving. Using in the feeding of animals the third group of premix P60-3/P throughout the entire dry period in combination with 1.2-propanediol is accompanied by the most pronounced reliable increase in the content of erythrocytes in these individuals on the third day after calving (by 21.3%, P<0.05). Two weeks before calving, the content of the total protein in the blood serum increases significantly (25.2-30.1%, P<0.01) and also albumins (21.7% (P<0.05) – 52.2% (P<0.01) The use of the third group of premix P60-3/P in the feeding of animals from the first day of the dry period is accompanied by the most pronounced reliable increase in the total protein content of these individuals two weeks before calving (30.1% P<0.01).

Keywords: cows, feed, blood, indicator, metabolism.
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Authors: 1Ezhkova A.M. – Doctor of Biological Sciences; 1Yapparov I.A. – Doctor of Biological Sciences; 1Ezhkov V.O. – Doctor of Veterinary Sciences; 1Yapparov A.Kh. – Doctor of Agricultural Sciences, professor; 2Faizrakhmanov R.N. – Candidate of Agricultural Sciences.
1Tatar Research Institute of Agricultural Chemistry and Soil Science – Subdivision of the Federal Research Center ซKazan Scientific Center of Russian Academy of Sciencesป (Tatar Research Institute of Agricultural Chemistry and Soil Science, FRC Kazan Scientific Center of RAS) (e-mail: niiaxp2@mail.ru; egkova-am@mail.ru).

2Bauman N.E. Kazan State Academy of Veterinary Medicine, Kazan (e-mail: kgavm_baumana@mail.ru).

The research data on the different dosage effectiveness of new generation feed supplement nanostructured sapropel for increasing the productivity and improving the hematological parameters of young cattle are presented. The objects of study were Kholmogory breed calves of Tatarstan type at the age of one month, production tests were conducted in the Atninskiy region of the Republic of Tatarstan. The calves were affected by nanostructured sapropel in doses of 3.0; 1.8; 0.6% and fodder sapropel at the optimal dose of 3.0% to the dietary dry matter. Supplementary feeds had been injected for 60 days. Experiments proved that the use of fodder sapropel increased the living weight of calves by 3.3 kg, when using nanostructured sapropel - by 6.0; 7.7 and 7.1 kg, in comparison with control animals. The use of sapropels promoted an increase in the red blood cells number in the calves’ blood, the hemoglobin content, total calcium, inorganic phosphorus, total protein and alkalinity reserve index, which did not exceed the physiological norm. The nanostructured sapropel was more effective in animals than fodder sapropel. The best results for the living weight and optimization of blood parameters were achieved when using the nanostructured sapropel at a dose of 1.8%. The long-term nanostructured sapropel injection at a dose of 3.0% to the calves diet showed a high efficiency of average daily gain in the first half of the experiment, and then excessive nutrients supply led to a decrease in living weight. The nanostructured sapropel use at a dose of 0.6% improved productivity and hematological parameters, but did not reach the potential of larger dose. The supplementary feed based on sapropel can be recommended for replenishment of protein and mineral nutrition of animals.

Keywords: calves, supplement feed, nanostructured sapropel, average daily gain, morphological and biochemical parameters of blood.
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