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¹ 1, 2017

Ńźą÷ąņü ķīģåš ęóšķąėą ā ōīšģąņå .pdf (2,76 ĢĮ)
  Toxicology
   
Title:

CYTOGENETIC CHANGES IN LUNG EPITHELIAL CELLS OF BOVINE FETUS UNDER THE INFLUENCE OF PESTICIDE FROM SYNTHETIC PYRETHROID GROUP

   
Authors: Valiullin L.R. – Candidate of Biological Sciences; Egorov V.I. – Candidate of Biological Sciences; Shangaraev N.G. – head of laboratory; Idiyatov I.I. – Candidate of Biological Sciences; Tremasov M.Ya. – Doctor of Biological Sciences, professor; Papunidi K.Kh. – Doctor of Veterinary Medicine, professor; Nikitin A.I. – Candidate of Veterinary Sciences.
   
Address:
Federal Center for Toxicological, Radiation and Biological Safety, Kazan (e-mail: vnivi@mail.ru).

   
Abstract:
The cytogenetic analysis of lung epithelial cell line from bovine embryo was carried out after the exposure to pesticide from group of synthetic pyrethroids. The studied substance was added to the nutrient medium for cultivation of cells in various concentrations. The effect of a deltamethrin on cultural and morphological properties of cells was determined basing on the following parameters: viability coefficient, proliferation index, cytotoxicity index, mitotic index, ratio of dead cells. The experiments showed that exposure to deltamethrin results in lower survival rate of the epithelial cells lung line comparing to the control group. Comparison of mitotic indexes at influence of deltamethrin revealed that increase of the dosage of toxicant reduces proliferative activity of cells and considerably increases cytotoxicity. The study of dead cells ratio showed that exposure to deltamethrin at different concentrations showed an increased death rate at higher concentrations. Comparing cells morphology in a monolayer showed that exposure to deltamethrin in low doses resulted in surface changes of cells. A higher rate of the toxicant resulted in destruction of monolayer and the presence of a large number of the debris indicating high percent of death of cells than that of the controls. Basing on the obtained data the mechanism of toxic effect of synthetic pyrethroid was studied on cellular level and the results can be used for further studies on investigation of antidotes at poisoning with this pesticide.
   
Keywords: pesticides, synthetic pyrethroids, deltamethrin, epithelial cells lung, proliferation, cytotoxicity
   
References:
1. Krasnov, A.V. Ekologicheski chistaya selskohozyaystvennaya produktsiya – vazhnyiy faktor sohraneniya zdorovya natsii [Organic agricultural production – an important factor of preservation of health of the nation] / A.V.Krasnov // «Toksikozyi zhivotnyih i aktualnyie problemyi bolezney molodnyaka» - Animal toxicoses and current problems of young animal diseases: proceedings of International scientific conference, Kazan, October 25-27, 2006. – Kazan, 2006. – P. 6-9.
2. Larina, E.V. Veterinarno-sanitarnvya ekspertiza ekzoticheskih fruktov, yagod i ovoschey na nalichie nekotoryih pestitsidov [Veterinary and sanitation evaluation of exotic fruits, berries, and vegetables for presence of some pesticides] / E.V.Larina // Problemy veterinarnoy sanitarii, gigienyi i ekologii. – 2011. – ¹ 1(5). – P. 20-22.
3. Conant, J. Pesticides are poison / J.Conant // Hesperian Foundation. – 2005. – Š. 3-15.
4. Kenneth, D. Racke. Pesticide Residues in Food and International Trade: Regulation and Safety Considerations / D. Kenneth // Pesticide Chemistry: Crop Protection, Public Health, Environmental Safety. – 2007. – P. 29-41.
5. European Commission: Monitoring of pesticide residues in products of plant origin in the European Union, Norway, Iceland and Liechtenstein 2004, Commissions Staff Working Document. 2006. http://ec.europa.eu/food/fvo/specialreports/pesticides_index_en.htm.
6. Galyautdinova, G.G. Sochetannyie otravleniya zhivotnyih mikotoksinom, detsisom i kadmiem [Associated poisonings of animals with mycotoxin, detsis and cadmium] / G.G.Galyautdinova, V.I.Egorov, E.G.Gubeeva // «Aktualnyie problemyi veterinarnoy farmakologii, toksikologii i farmatsii» - current problems of veterinary pharmacology, toxicology, and pharmacy: proceedings from IV meeting of Russian pharmacologists and toxicologists. – Moscow, 2013. – P. 163-165.
7. Ivanov, A.V. Toksikologicheskaya bezopasnost – problemyi i puti ih resheniya [Toxicological safety – problems and ways of their solution] / A.V.Ivanov, M.Ya.Tremasov, K.H.Papunidi // proceedings from the 2nd meeting of Russian pharmacologists and toxicologists. - Kazan, 2009. – 620 p.
8. Problemyi zagryazneniya okruzhayuschey sredyi i toksikologiya [Problems of environmental pollution and toxicology] / edited by G. Wer. – Moscow: Mir, 1993. – 143 p.
9. Krutko, N.S. Obnaruzhenie ostatochnyih kolichestv deltametrina v kurinyih yaytsah [Detection of residual quantities of deltamethrin in eggs] / N.S.Krutko, N.A.Brichko, N.V.Blinov // Rossiyskiy zhurnal «Problemyi veterinarnoy sanitarii, gigienyi i ekologii». –2012. - ¹ 1 (7). – P. 26-27.
10. Ivanov, A.V. Obespechenie himicheskoy i biologicheskoy bezopasnosti zhivotnovodstva v sovremennyih usloviyah [Ensuring chemical and biological safety of livestock production in modern conditions] / A.V.Ivanov // Toksikozyi zhivotnyih i aktualnyie problemyi molodnyaka – Animal poisonings and current problems of young animal diseases: proceedings from the International scientific conference. – Kazan, 2006. – P. 9-12.
11. Chawia, R. Acute oral toxicity studies with K-othrine (asiyn the ticpyrethroid insecticide) in Swissal binomice / R.Chawia // UttarPradesh. J. Zool. - 1996. – V. 16. - ¹1. – Š.67-68.
12. Extension Toxicology Network: Pesticide information profiles. Deltamethrin. Revised 9/95, 1996 Available at: http://extoxnet.orst.edu/pips/deltamet.htm. Accessed: Jan 21, 2009.
13. The European Agency for the Evaluation of Medicinal Products: Veterinary Medicines and Information Technology. Committee for Veterinary Medicinal Products Deltamethrin Summary Report 3, EMEA/MRL/779/01-Final, 2001.
14. World Health Organisation: Environmental Health Criteria: 97. ISBN: 92 4 154297 7, 1990.
15. International Programme on Chemical Safety (IPCS): Deltamethrin UKPID Monograph. Available at: http://www.inchem.org/documents/ukpids/ukpids/-ukpid62.htm. Accessed: Oct 09, 2008.4.
16. Lukyanov, A.S. Bioetika. Alternativa eksperimentam na zhivotnyih [Bioethics. An alternative to experiments on animals] / A.S.Lukyanov, L.L.Lukyanova, N.M.Chernavskaya i dr. // M.: Izd-vo MGU, 1996. – 253 s.
17. Clemendson, C. MEIC evaluation of acute systemic toxicity / C.Clemendson, E.E.Mc Farlane-Abdulla, M.Anderson et al. // ATLA. - 2002. – V.24. - Suppl. - Š. 251-311.
18. Senmartin, C. Evaluation of the in vitro efficacy of permethrin and deltamethrin on Aedesalbopictus / C.Senmartin, E.Bouhsira, E.Lienard [et al.]. // Revuede MedecineVeterinaire. – Ņ. 166. – V. 5-6. – P.170-174.
19. Sidorin, G.I. Ob «alternativnyih» metodah issledovaniya v toksikologii [About "alternative" methods of research in toxicology] / G.I.Sidorin, A.D.Frolova, L.V.Lukovnikova // Abstracts from the 1st meeting of Russian toxicologists. – Moscow, 1998. – 317 p.
20. Nabatov A.A., Raginov I.S. The DC-SIGN-CD56 interaction inhibits the anti-dendritic cell cytotoxicity of CD56 expressing cells. InfectAgentCancer.2015 Dec 10;10:49. doi: 10.1186/s13027-015-0043.
21. Horn, T. Acute hepatotoxicant exposure induces TNFR – mediated hepatic inuri and cytokine/ apoptic gene expretion / T.Horn, T.Brien, L.Schook, M. Rutherford // Toxicol. Sci. – 2000. - V. 54. - ¹1. – Š. 262-273.
22. Dyakonov, L.P. Zhivotnaya kletka v kulture (Metodyi i primenenie v biotehnologii) [Animal cells in culture (Methods and application in biotechnology)] / edited by L.P.Dyakonova – Moscow: Izdatelstvo «Sputnik», 2009. – 656 p.
  Infectious pathology
   
Title:

METHOD FOR PRODUCING HYPERIMMUNE SERUM AGAINST BOVINE PARAINFLUENZA-3 AND ESSESMENT OF ITS THERAPEUTIC EFFICIENCY

   
Authors: Budulov N.R. – Doctor of Veterinary Medicine; Salikhov Yu.S. – Candidate of Veterinary Sciences; Musaeva M.N. – Candidate of Veterinary Sciences; Karpuschenko K.A. – Candidate of Veterinary Sciences; Gaydarbekova Kh.M.
   
Address:
Caspian Zonal Research Veterinary Institute, Dagestan Republic (e-mail: budulov1951@mail.ru).

   
Abstract:
In the farms of Dagestan Republic a wide spread of viral respiratory diseases of polyetyological character was registered. Of all the animals tested the number of parainfluenza-3 seropositive calves was 66.9% of the examined animals, infectious bovine rhinotracheitis – 37.8%, adenovirus – 14.1%, bovine viral diarrhea – 11.7% and respiratory syncitial virus – 7.8%. The aim of the research was to develop a method for producing calve hyperimmune serum against parainfluenza-3 virus and trial its therapeutic and prophylactic efficacy. The hyperimmune serum was obtained by immunization of animal donors with 6-fold associated intranasal, subcutaneous, and intravenous administration of "Paravak" live vaccine against cattle parainfluenza-3 with virus titer 10 5.0TTsD 50/ml with ascending doses and intervals of 7 days. Before the immunization the immune system was activated using levamisole in dose of 3 mg/kg of body weight, three times, with 2-days interval. This promoted the production of parainfluenza antibodies in higher titers (1:1.024±2.43), than in animals immunized with sodium nucleinat and pyrogenal. The carried out investigations resulted in development of a method producing hyperimmune serum for treatment and prophylaxis of cattle parainfluenza-3. The subcutaneous administration of hyperimmune serum in association with intranasal droplet application onto nasal mucosa with prophylactic purpose prevents the their infection in critical age period (at 1 to 3 months after birth), thus reducing the death rate for almost 7 times. The drug is safe, active and demanded by the practice. In the group of calves, treated with the hyperimmune serum with on prophylactic purpose the deviation was 3.1%, which is for 6.9 times lower, compared with animals, that were not subjected to the preventive treatment. Treatment of sick calves by serum resulted in the recovery of 92.2% of the animals, while at using the conventional methods of treatment the recovery rate was 78.6%. The significance and originality of the method for producing the hyperimmune serum against cattleparainfluenza-3 is protected by RF patent.
   
Keywords: cattle young herds, viral respiratory infections, seroconversion, hyperimmunisation, antibodies, blood serum, parainfluenza-3 virus, Dagestan Republic
   
References:
1. Budulov, N.R. Vyyavlenie respiratornykh virusnykh infektsiy molodnyaka KRS v khozyaystvakh Dagestana [Detection of viral infections in young cattle herds in farms of Dagestan] / N.R.Budulov // Veterinariya i kormlenie. – 2014. – Vol. 3. – P. 18–20. 
2. Gryazin, V.N. Etiologiya respiratornykh zabolevaniy telyat i mery spetsificheskoy profilaktiki v usloviyakh spetskhoza [The etiology of respiratory diseases of calves and specific prevention measures in special facilities] / V.N.Gryazin // Nauchno-tekhnicheskiy bulleten IEVSiDV. – Novosibirsk. – 1982. – Issue 11. – P. 19–22. 
3. Patent RF 2396979, IPC C2, A61K 39/395, A61P 31/12. Giperimmunnaya polivalentnaya syvorotka protiv massovykh virusnykh bolezney krupnogo rogatogo skota [Hyperimmune polyvalent serum against mass virus disease in cattle] / V.A.Sergeev, O.V.Sergeev; applied and owned by Sergeev Vitalij Aleksandrovich i Sergeev Oleg Vitalevich - 2008130944/15; applied on 29.07.2008; published on 20.08.2010. Bul. 23. – 5 p.
4. Patent RF 2587323, IPC C1, A61K 39/155, A61P 31/14. Sposob polucheniya giperimmunnoy syvorotki protiv paragrippa-3 krupnogo rogatogo skota [Method of producing the hyperimmune serum against cattle parainfluenza-3]/N.R.Budulov, Yu.S.Salikhov, M.N.Musaeva, Kh.M.Gaydarbekova, K.A.Karpushchenko; applied and owned by FGBNU "Prikaspiyskiy zonalnyy NIVI" - 2015102255/15; applied on 26.01.2015; published on 20.06.2016. Bul. 17. – 6 p.
5. Poluchenie giperimmunnoy tsitratno-fenolizirovannoy krovi i ispytanie eyo pti paragrippe-3 telyat [Preparation of hyperimmune citratephenolized blood and its trial against cattle parainfluenza-3] / A.A.Sviridov [et al.] // Nauchno-tekhnicheskiy bulleten IEVSiDV. – Novosibirsk. – 1978. – Issue 10. – P. 6–9.
6. Rasprostranenie infektsionnogo rinotrakheita i virusnoy diarei-bolezni slizistykh obolochek krupnogo rogatogo skota v razlichnykh regionats Rossii [The spread of bovine rhinotracheitis and bovine viral diarrhea among cattle herds in different regions of Russia] / K.P.Yurov [et al.] // Trudy VIEV. – 2003. – Vol. 73. – P. 15–22. 
7. Sistema veterinarno-sanitarnykh, profilakticheskikh i lechebnykh meropriyatiy protiv infektsionnykh bolezney krupnogo rogatogo skota v khozyaystvakh Rossiyskoy Federatsii [The system of animal health, preventive and therapeutic measures against cattle infectious diseases in farms in the Russian Federation] / M.I.Gulyukin [et al.]. – Moscow – 2007. – 14 p. 
8. Etiologicheskaya struktura massovykh respiratornykh bolezney molodnyaka krupnogo rogatogo skota v khozyaystvakh, zanimayushchikhsya proizvodstvom moloka [The etiological structure of mass respiratory diseases of young cattle in diary farms] / G.A.Glotov [et al.] // Sibirskiy vestnik selskokhozyaystvennoy nauki. – 2008. – Vol. 3. – P. 72–78. 
9. Effektivnost syvorotochnykh preparatov pri virusnykh respiratornykh zabolevaniyakh telyat [The efficiency of using serum-based drugs atviral respiratory diseases of calves] / S.A.Zhidkov [et al.] // Byulleten VIEV. – 1976. – Is. 22. – P. 38–41. 
10. Babiuk L.A., Van Drunen Littel-van den Hurk S., Tikoo S.K. Immunology of bovine herpesvirus 1 infection. Vet. Microbiol. – 1996. – Vol. 53. – ¹ 1–2. – Š. 31–42. 
11. Jericho K.W., Jericho K.W., Kozub G.C. Experimental infectious respiratory diseases in groups of calves: lobar distribution, variance, and sample-size requirements for vaccine evaluation. Can. J. Res. – 2004. – Vol. 68. – ¹ 2. – P. 118–127. 

   
   
Title:

A METHOD OF PRODUCING PORCINE LEUKOCYTE SUSPENSION FOR BIOREACTOR-BASED CULTIVATION OF AFRICAN SWINE FEVER VIRUS

   
Authors: Zakutskiy N.I. – Doctor of Veterinary Medicine; Shirockova T.G. – Processing Engineer; Neverovskaya N.S. – Candidate of Biological Sciences; Yurkov S.G. – Doctor of Biological Sciences; Balysheva V.I. – Doctor of Biological Sciences.
   
Address:
Russian National Research Institute for Veterinary Virology and Microbiology (e-mail:vniivvim@mail.ru).

   
Abstract:
The article proposes a novel technology of production of porcine blood leukocyte suspension for cultivation of African swine fever (ASF) virus culture in bioreactors. Porcine blood plasma was used as basic cell material. Plasma was obtained in a slaughterhouse using a special system comprising devices for porcine blood sampling and a load of supernatant fraction of blood plasma into glass bottles. The porcine leukocyte suspension prepared from plasma showed positive results during the development of production technique for ASF virus culture in bioreactors of various capacities. The comparative studies showed that at cultivation in suspension from porcine bone marrow (PBM) or blood leukocyte (PL) cell suspension in laboratory fermenters the ASF virus had similar infection and antigenic activity levels that was 7.5-8.0 lg HAU50/cm3 and 1:3125, respectively. The maximum accumulation of virus in a suspension of hematopoietic cells and PL was at cultivation concentration of 2.8 to 3.2 (PBM) and 5.0 to 6.0 (PL) million cells/cm3 and the multiplicity of infection of 0.01 to 0.1 (PBM) and 0.0001 to 0.001 (HP) HAU50/cell. The virus, cultivated in the LC suspension in similar conditions using fermenters with a capacity of 100 and 130 liters, also accumulated with high virulence and antigenic activity levels (7.53 ± 0.12 lg HAU50/cm3 and 1:625 to 1:3125, respectively). Therefore, an opportunity of replacing the costly and time-consuming PBM culture with a more economical and technologically advanced PL cell system prepared from porcine blood in a slaughterhouse to be used for ASF virus large scale growth was shown.


   
Keywords: virus, ASF, PBM (porcine bone marrow cells), PL (porcine leukocytes), infection/antigenic activity, bioreactor
   
References:
1. Immunobiologicheskiye svoystva attenuirovannogo shtamma FK-135 virusa afrikanskoy chumy sviney, vyraschennogo v suspenzii kletok kostnogo mozga sviney [Immune and biological properties of ASF virus attenuated FK-135 strain cultured in suspension of porcine marrow cells] / N.I. Zakutsky, T.G. Shirokova, S.G. Yurkov, V.M. Balyshev // Agricultural Biology. – 2014. – Vol.4. – P.70-74.
2. Effektivnost razlichnyh metodov vyrashnivaniya virusa arikanskoy chumy sviney v gemopoeticheskih kletkah [The efficacy of various methods of ASF virus cultivation in hemopoetic cells] / N.I.Zakutsky, T.G.Shirokova, N.S.Neverovskaya, S.G.Yurkov // Agricultural Biology. – 2014. – Vol.4. – P.58-64.
3. Nekotoriye osobennosti masshtabirovaniya protsessa vyraschivaniya raznyh kultur kletok (KMS, BHK-21) i virusov (AChS, blutanga) [Some peculiarities of scaling the cultivation process for various cell cultures (KMS, BHK-21) and viruses (ASF, bluetongue) / N.I.Zakutsky [et al.] // Veterinarny vrach. – 2015. – Vol. 1. – P.3-8.
4. Slivko, V.V. Usovershenstvovaniye tehnologii izgotovleniya inaktivirovannoy vaktsiny protiv blutanga [Improving production technology for inactivated vaccine against bluetongue]: dissertation for candidate of Veterinary sciences / V.V.Slivko. – Pokrov, 2003. – 148p.
5. Nesterov, Å.Ą. Soveshenstvovaniye tehnologii iizgotovleniya inaktivirovannoy vaktsiny protiv blutanga i otsenka yeyo effektivnosti [Improving production technology for inactivated vaccine against bluetongue and evaluation its efficacy]: abstract from dissertation for Candidate of Veterinary sciences/ E.A. Nesterov. – Pokrov,2012. – 27p.
6. Sergeev, V.A. Reproduktsiya i vyraschivaniye virusov: monografiya [Virus reproduction and cultivation: manuscript] / V.A.Sergeev. – Moscow: Kolos, 1976. – 304p.
7. Patogenniye svoystva kulturalnogo virusa afrikanskoy chumy sviney [Pathogenic and immunogenic properties of ASF cultivated virus] / E.I.Prudnikova, V.M.Balyshev, S.A.Belyanin, V.I.Balysheva // Proceedings from Int. sci. conf. dedicated to 55th anniversary of Russian national Research Institute of Veterinary Virology and Microbiology. – Pokrov, 13th Nov., 2013. – Pokrov, 2013. – P.54-57.
8. Adaptatsiya virusa afrikanskoy chumy sviney k perevivayemym liniyam kletok [ASF virus adaptation to cultivated cell lines] / Yu.P.Morgunov [et al.] // Veterinariya. – 2013. – Vol.11. – P.58-62.
9.Burlakov, V.A. Immunobiologicheskiye svoystva virusa i problema razrabotki sredstv spetsficheskoy profilaktiki afrikanskoy chumy sviney [Immune and biological features of ASF virus and the problem of development of specific prevention measures against ASF]: dissertation of Doctor of veterinary sciences / V.A.Burlakov. – Pokrov, 1979. – 478p.
10. Kulturalniye i immunobiologicheskiye svoystva attenuirovannyh variantov virusa AChS, vydelennyh iz prirodnyh izolyatov vozbuditelya v HPK [Cultural, immune and biological features of attenuated variants of ASF virus isolated from natural isolates in HPK]/ A.V.Kiselev [et al.] // Voprosy veterinarnoy virusologii, mikribiologii i epizootologii – Issues of veterinary virology, microbiology, epizootology: proceedings from sci. conf. – Pokrov: Russian national Research Institute of Veterinary Virology and Microbiology, 1992. – P.44-45.
11. Patent 2452511 Russian Federation, IPC A61K 39/187, C12N 7/00. Attenuirovanny shtamm virusa afrikanskoy chumy sviney 2-go serotipa dlya razrabotki diagnosticheskih i vaktsinnyh preparatov [Attenuated strain ofASF 2nd serotype for development of diagnostic and vaccine preparations] / Yu.F.Kalatenko, V.I.Zhesterev, V.A.Mischanin, V.M. Balyshev; applied and owned by Russian national Research Institute of Veterinary Virology and Microbiology. – N 2010146542/10; applied on 16.11.2010; published 10.06.2012, Bulletin # 16. – 7 p.

  Non-transmittable diseases
   
Title:

THE DYNAMICS OF SWINE HEMATOLOGICAL PARAMETERS AT PREVENTION OF ALIMENTARY ANEMIA

   
Authors: Andreyeva A.V. – Doctor of Biological Sciences, professor; Nikolayeva O.N. – Candidate of Biological Sciences.
   
Address:
The Bashkir State Agrarian University, Ufa (e-mail: alfia_andreeva@mail.ru).
   
Abstract:
The iron deficiency-borne anemia of pigs is a pathological state of body characterized by disorders in hemopoietic organs and metabolism and subsequently it results in lower growth rate and to decrease in immunity. In this regard, the aim of this work was to study dynamics of hematological parameters in pigs at prevention of alimentary anemia using modern ferriferous drugs “Ferraminal-100”, “Suiferrovit”, “Ferran”. In farrow department of swine factory farm 120 weaning piglets were selected and divided into four groups with 30 piglets in each group. The first group was control (without use of medicines); piglets in the second group were injected the drug “Ferranimal-100” in a dose of 1.5 ml at 4 days after birth and at 15 days in a dose of 3.0 ml per animal; the third group received “Suiferrovit” intramuscularlyat 4 days after birth in a dose of 5 ml and again at 10 days and before postweaning in 10 ml per animal; the fourth group received the medicine “Ferran” at 4 days after birth in 1.5 ml and again in two weeks in 2.0 ml per pig. Prior to the experiment (4 days) and after its termination (at 26 days) pigs in all the groups were tested for iron deficiency, total protein, hemoglobin, hematocrit, number of erythrocytes. Hematological blood analyses were conducted using Medonic CA620/530 hematological analyzer. In the control animals lower level of hemoglobin (60.4±0.28 g/l) and erythrocytes (3.28±0.16õ1012/l)was registeredat 26 days. In the second group after “Ferranimal-100” application the level of hemoglobin exceeded the background level for 1.47 times (109.3±2.5 g/l), the number of erythrocytes increased for 1.31 times (5.46±0.16õ1012/l), the hematocrit was above background values for 1.34 times. In the third group of animals the tendency to increase in the level of hemoglobin (for1.34 times), erythrocytes and hematocrit (for 1.36 times) was registered. In the fourth group the application of “Ferran” drug promoted increase in the level of hemoglobin, erythrocytes and hematocrit in relation to the background valuesfor 1.4; 1.31 and 1.29 times respectively. In all the three experimental groups tendency to increase of iron content in serum was registered. At 26 days exceed in relation to background values was for 1.51; 1.56 and 1.48 times respectively. Therefore, “Suiferrovit” and “Ferranimal-100” have expressed preventive effect at iron deficiency anemia in pigs.
   
Keywords: pigs, iron deficiency anemia, prevention, «Ferranimal-100», «Suiferrovit», «Ferran»
   
References:
1. Andreeva, A.V. Koncentracija immunoglobulinov v syvorotke krovi porosjat-otemyshej pri ispol'zovanii probiotika «Sporovit» i askorbinovoj kisloty [Concentration of immunoglobulins in weaned piglet blood at using “Sprovit” probiotic and Vitamin C] / A.V.Andreeva, E.T.Muratova // Sovremennye dostizhenija veterinarnoj mediciny i biologii - v sel'skohozjajstvennoe proizvodstvo. - Ufa, 2009. – P.104-106.
2. Bushov, A.V. Anemija molodnjaka svinej [Anemia in piglets] / A.V.Bushov, Je.V.Ten // Veterinarija sel'skohozjajstvennyh zhivotnyh. - 2007. – Vol. 10. – P. 45 - 49.
3. Krasnova, E.G. Deficit zheleza i anemija u porosjat [Iron deficiency and anemia in piglets] / E.G.Krasnova // Veterinarnyj vrach. – 2013. – Vol.10. – P. 54-55.
4. Medvedev, I.N. Krezacin i gamavit pri narushenijah gomeostaza u novorozhdennyh porosjat [Using krezacin and hemavit drugs at hemostasis disorders in newborn piglets] / I.N.Medvedev, A.V.Parahnevich // Veterinariya. – 2015. – ¹ 3. – P. 50-53.
5. Nikolaeva, O.N. Gematologicheskie pokazateli teljat pri ispol'zovanii kompozicii fitoprobiotikov i polisolej mikroj elementov [Blood parameters in calves at using a composition of phytoprobiotics and salts of microelements] / O.N.Nikolaeva // Problemy i perspektivy agrarnogo proizvodstva – problems and perspectives in agrarian production: int. sci. conf., Smolensk, December 31st - January 1st, 2007 – Smolensk, 2007. – P. 289-291.
6. Okolyshev, S.G. Zhelezodeficitnaja anemija porosjat [Iron deficient anemia in piglets] / S.G.Okolyshev // Zhivotnovodstvo Rossii. - 2013. – Vol.1. – P. 17-19.
7. Primenenie suiferrovita dlja profilaktiki zhelezodeficitnoj anemii porosjat [Using suiferrovit drug to prevent iron deficient anemia in piglets] / E.Ponomarev, E.Struchkova, V.Sorokin, O.Simonova // Veterinarija sel'skohozjajstvennyh zhivotnyh. – 2015. – Vol.1-2. – P.61-67.
8. Prohorova, I.A. Profilaktika zhelezodeficitnoj anemii porosjat [Prevention of iron deficient anemia in piglets] / I.A.Prohorova // Svinovodstvo. – 2013. – N 1. – P.47-49.
   
   
Title:

ANDROLOGICAL CLINICAL EXAMINATION OF DOGS IN DOG TRAINING CENTER

   
Authors: Perletskaya O.V. – postgraduate fellow; Shestakova A.N. – Candidate of Biological Sciences.
   
Address:
Vyatka State Agricultural Academy, Kirov (å-mail: perletzkaja@yandex.ru).

   
Abstract:
The aim of this work was to evaluate hematological parameters, ultrasound parameters of the reproductive organs and semen of dog males at andrological examination. Andrological clinical examination of dogs held in the kennel dog service of the Russian Federation Ministry of Internal Affairs across Kirov region during August and September, 2016. Male dogs of German shepherd breed at the age of 3-5 years old were studied during the experiment. Semen evaluation included determination of volume, density, concentration and pH, and sperm activity and morphology was studied by calculating the ratio of live and dead forms. The blood serum was studied for total protein, the amount of urea, creatinine, the activity of alaninaminotransferase (ALT) and alkaline phosphatase levels of testosterone. Using ultrasonic scanner set the size of the testes and prostate was determined. The examination of the obtained sperm from the dogs showed that the volume of ejaculate on average was 7.64±0.7 ml, and its concentration was 0.25±0.01 billion/ml. A detailed assessment of sperm mobility determined that in 80% of sperm lipoprotein membrane was not damaged, and it prevented the dye to enter the cytoplasm of cells while 20% lacked this property. Of the investigated semen samples the number of pathological forms of germ cells on average was not higher than 3.2 per cent. The sperm had a slightly acid reaction medium (6.4±0.01). The research results showed that the level of testosterone in males was low (20.48±5.6 µmol/l), and the activity of the sperm cells was higher than the average normal values (9.85±0.1 points). In German shepherd dogs the prostate size is 5.8 x 4.3 cm, right testis is 5.4×2.7 and the left is 4.8 × 2.7 cm. In the peripheral blood of German shepherd dogs the number of leukocytes was 12.5×109/l, number of erythrocytes was 6.91×1012/l, number of platelets was 268×109/l, total protein was 72.0 g/l, urea was 6.6 mmol/l, creatinine was 108 µmol/l and testosterone was 20.4 µmol/l. Activity of alaninaminotransferase corresponded to 32.0 U/l, and alkaline phosphatase was 52.0 U/L.
   
Keywords: semen analysis, male-producers, testosterone, prostate
   
References:
1. Allen, V.E. Polny kurs akusherstva i ginekologii sobak [A full course of dog obstetrics and gynecology]. - Moscow: LLC "Aquarium-Print". - 2006. - 448p.
2. Bellwood, B. Laboratory procedures. Techniques for conducting tests and analyses. / B. Bellwood, M. Andrasik-Catton: Moscow: Aquarium. – 2014. – 142 p.
3. Goncharov, V.P. Osnovniye pokazateli i otsenka kachestva spermy proizvoditeley zhivotnyh. Metodichesliye ukazaniya [Main parameters and evaluation of semen quality in production animals. Methodical guidelines]. – Moscow: 2003. – 19 p.
4. Dunn, D. Cytology in dogs and cats. – Moscow, 2014. - 255 p.
5. Simpson, G. Manual of reproduction and neonatology dogs and cats. / John.Simpson, G. England, Moscow: Sofian. – 2005. – 267 p.
6. Fedotov, S. V. Sovershenstvovaniye reproduktsii slzhebnyh sobak v usloviyah CKS MVD Rossii [Improvement of dogs reproduction in dog training centers of the Russian Federation Ministry of Internal Affairs / Bulletin of the Altai state agrarian University. – ¹ 11 (121). - 2014. – P. 116-120.
7. Feldman E. Endocrinology and reproduction in dogs and cats. / Feldman E., R.Nelson, Moscow: Sofian. – 2008. – 1242 p.

  Parasitology
   
Title:

THE EFFECT OF PATHOGENETIC THERAPY AT SHEEP SARCOCYSTIS INFECTION ON MUTTON QUALITY

   
Authors: 1Pozov S.Ą. – Doctor of Veterinary Medicine, professor; 1Porublev V.A. – Doctor of Biological Sciences, professor; 2Orlova N.E. – Candidate of Veterinary Sciences; 1Sotnikova T.V. – graduate student; 1Calugnaya V.A. – graduate student.
   
Address:
1Stavropol State Agrarian University, Stavropol (e-mail: fvm-fvm@yandex.ru).
2Russian State Agrarian Correspondence University, Balashiha (e-mail: nadorlov@mail.ru).

   
Abstract:
During the recent years the studies showed that mutton from sheep infected with sarcocysts has lower shelf life, the meat is usually contaminated with the infectious microflora, nutritional value is significantly lower, has a gray shade and the lowered density. The aim of our research is to study the effect of sarcocysts on morphological and biochemical parameters of mutton from sheep challenged with sarcocysts. The experiments were carried out on 20 sheep of the Stavropol breed; of them 15 animals were infected with sarcorcystists, and 5 sheep were free from sarcocysts. The infected sheep were divided into three groups (with 5 animals in each group). At 3 months after the experiments began the animals were euthanized and their meat was sampled for microscopic and biochemical studies. The investigation results showed that the sheep infected with sarcocysts and not subjected to pathogenetic therapy were considerably behind in growth and development, average daily gain was 43.5 g, or for 3.1 times lower than the parameters of healthy animals. Their meat had more moisture for 6.3±1.36%, lower level of protein for 2.8±0.3%, fat - for 3.5±1.3%, myoglobin - for 90 units of optical density, ash - for 0.05±0.008%, calories - for 183.47 kJ in comparison with the parameters of healthy animals. Meat from the sheep with sarcocyst infection contained 36.9±3.0% of amino acids, including replaceable – 23.0±2.8%, irreplaceable – 13.9±0.2 ones that is respectively for 5.14%, 1.9% and 3.23% lower than the parameters of healthy animals. The level of threonine, methionine, isoleucine, lysine, glutamine acid and a tyrosine was significantly lower. Response to peroxidase was negative, formol test was positive, concentration of hydrogen ions was within limits of 6.3-6.6 that can be explained by a lower level of glycogen. Therefore such meat could not be stored for a long time. Processing with Sulfapyridazinum-natrium of the sheep infected with sarcocysts releases them from sarkotsist infection. However, within three months of the functions of organs and systems in the sheep which had had sarcocysts did not fully recovered and remained under the norm values therefore the average daily gain was 1.8 times lower, and the meat quality was considerably low than that of the intact animals. The complex treatment of the infected animals with sulfapirida-ziny sodium and a phytobiostimulator (FBS) allows rapid normalizing the functions of organs and systems that considerably increases growth rate, animal performance, improves meat biochemical structure and quality.
   
Keywords: sarcocystis, myoglobin, amino acid, development, growth rate, biochemical composition, biochemical content
   
References:
1. Nikolsky, S.N. Effectovnost sylfanpiridazina natriia pri sarkocistoze ovec [Efficiency of using Sulfapyridazinum-natrium at sheepsarcocyst infection] / S.N.Nikolsky, S.A.Pozov // Proceedings from the III meeting of VOPR. – Vilnius, 1982. - P. 2-4.
2. Pozov, S.A. K voprosy epizootologii i terapii sarkocistoza ovec [On the issue of epidemy and therapy of sheep sarcocyst infection] // Nauchniye Trudy Stavropolskogo SHI. – 1982. – Vol.5, Issue 45. – P. 3-5.
3. Pozov, S.A. Effektivnost sylfanpiridazina natriia pri sarkocistoze ovec [Efficiency of using sodium Sulfapyridazinum-natrium at sheep sarcocyst infection] / S.A.Pozov// Informacionnyi listok ¹451-82. – Stavropol: Stavropolsky CNTI, 1982. – P.3-5.
4. Pozov, S.A. Chimioterapia i therapia pri sarkocistoze ovec [Chemotherapy and therapy atsheep sarcocyst infection] / S.A.Pozov // Diagnostica, lechenie i profilactica infekcionnih i parazitarnyx zabolevanii zhivotnyh – Detection, treatment, and prevention of animal parasitic diseases: a collection of articles. - Stavropol: SHI. – 1984. – P. 4-6.
5. Pozov, S.A. Diagnostica i mery borby s sarkocistozom ovec: monografiya [Detection and control measures against sheep sarcocyst infection: manuscript]. – Stavropol: Stavropolskoye knigoizdatelstvo, 1985. – 136p.
6. Pozov, S.A. Profilactica sarcocystoza ovec s primeneniem sulfapiridazina natria [Prevention of sheep sarcocyst infectionusing Sulfapyridazinum-natrium ] / S.A.Pozov // Diagnostica, lechenie i profilactica infekcionnih i parazitarnyx zabolevanii zhivotnyh – Detection, treatment, and prevention of animal parasitic diseases: a collection of articles. - Stavropol: SHI. – 1985. – P. 3-6.
7. Pozov, S.A. Parasitocenos ovec i ego terapia [Sheep parasicenosis and its therapy] / S.A.Pozov // Informacionnyi listok ¹ 437-86. – Stavropol: Stavropolsky CNTI, 1982. – P. 4-7.
8. Pozov, S.A. K voprosy diagnostiki i terapii sarkocistoza ovec [On the issue of detection and therapy of sheep sarcocyst infection] / S.A.Pozov // Diagnostica, lechenie i profilactica infekcionnih i parazitarnyx zabolevanii zhivotnyh – Detection, treatment, and prevention of animal parasitic diseases: a collection of articles. - Stavropol: SHI. - 1986. – P. 7-10.
9. Pozov, S.A. Terapevticheskaya effektivnost oriprima i mebendazola pri sarkocistoze ovec [Therapeutic efficacy of oriprim and mebendazol atsheep sarcocyst infection] / S.A.Pozov // Diagnostica, lechenie i profilactica infekcionnih i parazitarnyx zabolevanii zhivotnyh – Detection, treatment, and prevention of animal parasitic diseases: a collection of articles. - Stavropol: SHI. - 1987. – P. 4-6.
10. Pozov, S.A. Terapia sarcocystoza ovec [Therapy of sheep sarcocyst infection] / S.A.Pozov // Abstracts of reports from the IV USSR meeting protozoologists. - Leningrad, 1987. – P.1-5.
11. Nikolsky, S.N. Sarcocystoz ovec. (Monographia) [Sheep sarcocyst infection: manuscript] / S.N.Nikolsky, S.A.Pozov. - Stavropol: Stavropolskoye knigoizdatelstvo, 1989. – 112 p.
12. Pozov, S.A. Sarcocystoz ovec (epizootiologia, clinicheskaya kartina, parasitocenosi, diagnostica, therapya i profilactica) [Sheep sarcocyst infection (epidemics, clinics, parasitocenoses, detection, therapy, and prevention]: abstract from dissertation for Candidate of Vet.Sci. / S.A.Pozov. - Leningrad, 1990. – 47 p.
13. Pozov, S.A. Soderzhanie mikroelementov v organizme i tcanyah ovec, porazhennih sarkocistami v associacii s bronhopnevmoniei [The level of microelements in sheep body and tissues affected with sarcocyst in association with bronchopneumonia] / S.A.Pozov, S.A.Eziev, V.A.Shalygina // Diagnostica, lechenie i profilactica zabolevanyi selskohozaistvennyh zhivotnyh-Detection, treatment, and prevention of farm animal diseases: a collection of articles. - Stavropol: Agrus. - 2009. – P. 79 -80.

  Zootechny
   
Title:

THE COMPARATIVE CHARACTERISTICS OF PRODUCTION BULLS WITH VARIOUS DNA-MARKERS ON DAIRY EFFICIENCY OF FEMALE ANCESTORS

   
Authors: 1Sibagatullin F.S. – Doctor of Veterinary Sciences, professor, 1Shaydullin R.R. – Candidate of Agricultural Sciences; 1Sharafutdinov G.S. – Doctor of Agricultural Sciences, professor; 2Faizov T.H. – Doctor of Veterinary Medicine, professor; 3Zaripov F.R. – Candidate of Biological Sciences; 4Shakirov S.K. – Doctor of Agricultural Sciences, professor.
   
Address:
1Kazan State Agrarian University, Kazan (e-mail: info@kazgau.ru).
2Federal Center for Toxicological, Radiation and Biological Safety, Kazan (e-mail: vnivi@mail.ru).
3The parent breeding enterprise «Elite», Russia (e-mail: elita-tatarstan@mail.ru).
4Tatar Research Institute of Agriculture, Kazan (e-mail: tatniva@mail.ru).

   
Abstract:
The aim of the work was to study production bulls of dairy breeds at breeding farms, the Republic of Tatarstan, with various genotypes of DNA markers on dairy efficiency of female ancestors. The study of RIB parameters in bulls showed that of cows of the Tatarstan type of holmogorsky breed with genotypes CSN3-AA, DGAT1-AK, LGB-BB, PRL-BB, GH-LL, TG5-CC, LEP-CC prevailed on milk yield; the producers with genotype CSN3-BB, DGAT1-AK, LGB-AA, PRL-BB, GH-VV, TG5-CC, LEP-CC prevailed on fat milk. Bulls of black and motley breed with a genotype CSN3-AA, DGAT1-AK, LGB-BB, PRL-AA, GH-VV, TG5-TT, LEP-TC surpassed their counterparts with other genotypes in milk yield while the animals with genotype CSN3-AB, DGAT1-AK, LGB-AB, PRL-AB, GH-LV, TG5-TC, LEP-CC had advantage on fat content. Of the Holstein bulls the genotypes CSN3-AB, DGAT1-AA, LGB-AA, PRL-AB, GH-VV, TG5-TC, LEP-CC showed the best parameters of milk yield, and producers with genotypes CSN3-BB, DGAT1-AK, LGB-BB, PRL-AB, GH-VV, TG5-CC, LEP-CC had the best parameters on content of fat, and the animals with genotypes CSN3-BB, DGAT1-AK, LGB-AA, PRL-R, GH-LL, TG5-TT, LEP-TC – on protein level.

   
Keywords: the bull-breeder, rib, mother, milk yield, diary fat, diary proteint, genotype, allele, CSN3, DGAT1, LGB, PRL, GH, TG5, LEP.
   
References:
1. Ahmetov, T.M. Ispol'zovanie metodov marker-vspomogatel'noj selekcii v molochnom skotovodstve Respubliki Tatarstan [Ņhe Use of methods of marker-assisted selection in dairy cattle breeding of the Republic of Tatarstan]: abstract of dissertation for Doctor of Biology: 06.02.01 / Ahmetov Tahir Munavirovich. – Kazan, 2009. – 50 p.
2. Ganiev, A.S. Polimorfizm gena zhirnomolochnosti krupnogo rogatogo skota [Gene polymorphism of cattle diary fat] / A.S. Ganiev, R.R.Shajdullin // Uchenye zapiski Kazanskoj gosudarstvennoj akademii veterinarnoj mediciny im. N.Je. Baumana. – Kazan': KGAVM, 2015. - Vol. 224 (4). – P. 30-35.
3. Glazko, V.I. Vvedenie v DNK-tehnologii [Introduction to DNA technology] / V.I.Glazko, I.M.Dunin, G.V.Glazko, L.A.Kalashnikova. – Moscow:FGNU «Rosinformagroteh», 2001. – 436 p.
4. Dzhaparidze, G.M. Polimorfizm genov molochnyh belkov i gormonov u korov golshtinskoj porody [Genetic Polymorphism of milk proteins and hormones in Holstein cows]: abstract of dissertation for Candidate of Biol.Sci.: 06.02.07 / Dzhaparidze Grigorij Mihajlovich. – Lesnye Poljany, 2013. – 21 p.

5. Drozdov, E.V. Polimorfizm genov, svjazannyh s molochnoj produktivnost'ju krupnogo rogatog oskota [Polymorphism of the genes associated with cattle dairy productivity]: abstract of dissertation for Candidate of Biol.Sci.: 03.02.07 / Drozdov Evgenij Vladimirovich. – St. Petersburg, 2013. – 23 p.

6. Zakirov, I.R. Genealogija , plemennye kachestva, DNK-marker produktivnosti bykov-proizvoditelej tatarstanskogo tipa, cherno-pestroj i mjasnyh porod skota [Genealogy, tribal quality, DNA markers of productivity of sires of the Tatar type, black-and-white and beef breeds of cattle] / I.R.Zakirov, R.A.Haertdinov, Sh.K.Shakirov [et al.]. – Kazan': Centr innovacionnyh tehnologij, 2011. – 208 p.

7. Zakirov, I.R. Genofond bykov-proizvoditelej molochnyh i mjasnyh porod v Tatarstane [Ņhe Genepool of bulls in dairy and beef breeds in Tatarstan] / I.R.Zakirov, R.A.Haertdinov, F.R.Zaripov, Sh.K.Shakirov [i dr.] – Kazan': Centr innovacionnyh tehnologij, 2015. – 200 p.

8. Zinnatova, F.F. Testirovanie plemennogo krupnogo rogatogo skota po DNK-markeram molochnoj produktivnosti [Testing of breeding cattle according to the DNA markers of milk production]: abstract of dissertation for Candidate of Biol.Sci.: 06.02.07; 03.01.04 / ZinnatovaFaridaFatihovna. – Kazan', 2013. – 24 p.

9. Zinov'eva, N.A. Primenenie DNK-diagnostik i dlja analiza genov–kandidatov lokusov kolichestvennyh priznakov sel'skohozjajstvennyh zhivotnyh [The use of DNA diagnostics for the analysis of candidate genes for loci of quantitativetraits of farm animals] / N.A. Zinov'eva, E.A. Gladyr' [et al.] // Nauchnye trudy VIZh. – Dubrovicy, 2001. – Issue. 61. – P. 218-224.
10. Zinov'eva, N.A. DNK-diagnostika polimorfizma genov – belkov moloka krupnogo rogatogo skota [DNA diagnostics of polymorphism of gene – protein cattle milk] / N.A.Zinov'eva, E.A.Gladyr', O.V.Kostjunina // Metody issledovanij v biotehnologii sel'skohozjajstvennyh zhivotnyh. – Moscow, 2004. – P. 7-22.
11. Kravchenko N.A. Razvedenie sel'skohozjajstvennyh zhivotnyh [Breeding of farm animals] / N.A.Kravchenko. - Moscow: Kolos, 1973. - 486 p.

12. Kalashnikova, L.A. Vozmozhnosti ispol'zovanija DNK markerov produktivnyh kachestv  zhivotnyhv prakticheskoj selekcionnoj rabote [Ņhe possibility of using DNA markers of productive qualities of animals in practical breeding work] / L.A.Kalashnikova // Sovremennye dostizhenija i problemy biotehnologii sel'skohozjajstvennyh zhivotnyh. – Dubrovicy. – 2003. - P. 33-39.

13. Merkur'eva, E.K. Biometrija v selekcii i genetike sel'skohozjajstvennyh zhivotnyh [Biometrics in selection and genetics of farm animals] / E.K.Merkur'eva.- Moscow: Kolos, 1970.- 424 p.

14. Ruzhevskij, A.V. Ob organizacii ocenki bykov-proizvoditelej po potomstvu i shirokomu ih ispol'zovaniju [On the organization of the evaluation of sires on progeny and their wide use] / A.V. Ruzhevskij // Materialy vsesojuznogo soveshhanija – seminara po organizacii proverkii i spol'zovaniju proizvoditelej ocenennyh i ispol'zovaniju proizvoditelej ocenennyh pokachestvu potomstva. Moscow, «Kolos», 1965.

15. Ruzskij, S.A. Ispol'zovaniemetodakorreljaciipriocenkebykovpopotomstvu [Ņhe Use of correlation technique in the evaluation of bulls through progeny] / S.A. Ruzskij. – TrudyVSHIZO, Issue. 10. - 1962.

16. Sibagatullin, F.S. Ispol'zovanie DNK tehnologij v zhivotnovodstve [Ņhe Use of DNA technologies in animal husbandry] / F.S. Sibagatullin, T.H. Faizov, G.S. Sharafutdinov, Sh.Z. Validov, R.R. Shajdullin // Vestnik Kazanskogo gosudarstvennogo agrarnogo universiteta..- 2010. - ¹ 1 (15). – P. 130-132.

17. Habibrahmanova, Ja.A. Polimorfizm genov molochnyh belkov i gormonov krupnogo rogatogo skota [gene polymorphism of milk proteins and hormones in cattle]: abstract of dissertation for kand. biol. nauk: 06.02.07 / Habibrahmanova Jazilja Aminovna. - LesnyePoljany: VNIIplem, 2009. - 19 p.

18. Shajdullin, R.R. Ocenka polimorfizma gena kappa-kazeina u zhivotnyh cherno-pestroj porody [Evaluation of genepolymorphism of Kappa-casein from animals of black-motley breed] / R.R. Shajdullin, A.S.Ganiev // Vestnik Ul'janovskoj gosudarstvennoj sel'skohozjajstvennoj akademii. – Vol. 3(31). – 2015. – P. 104-109.      

19.  Shakirov, Sh.K. Vlijanie porody i genotipa po genam CSN3, DGAT, PRL, LGĀ na molochnuju produktivnost' krupnogo rogatogo skota [Influence of breed and genotype for CSN3 genes, DGAT, PRL, LGĀ on milk productivity of cattle] / Sh.K. Shakirov, Ju.R. Jul'met'eva, F.F. Zinnatova // VestnikRASHN. – 2012. – ¹ 5. – P. 65–67.

20.  Jernst, L.K. Principy i metody ocenki bykov- proizvoditelej po kachestvu potomstva [Principles and methods of evaluation of bulls on quality of posterity] / L.K. Jernst // Mezhdunarodnyj sel'skohozjajstvennyj zhurnal. – 1965. - ¹ 2. - P. 6-8.

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25. Pawar R.S. Growth hormone gene polymorphism and its association with lactation yield in dairy cattle / R.S. Pawar, K.R. Tajane, C.G. Joshi, B.P. Bramkshtri // Indian J. of Anim. Sci. –2007. – 77 (9). – P. 94-98.


   
   
Title:

AN EXPERIMENT OF QUAIL BREEDING WITH USING POZITRON-3 AIR1 OZONATOR AND "BASULIFOR" PROBIOTIC ADDITIVE

   
Authors: 1Alekseev I.A. - Doctor of Veterinary Medicine, professor; 2Kadikov I.R. - Candidate of Biological Sciences; 1Ishtudova E.R. - postgraduate.
   
Address:
1Chuvash State Agricultural Academy, Cheboksary (e-mail: info@academy21ru).
2Federal Center for Toxicological, Radiation and Biological Safety, Kazan (e-mail:vnivi@mail.ru).

   
Abstract:
One of the most productive and economically efficient branch in poultry breeding is a quisl breeding. Room microclimate is of special significance in quail breeding. The work paper presents the results of using national brand artificial Ozonator "Pozitron-3 Air-1" and "Basulifor" probiotic feed additive in poultry farms for breeding young quails. It was found that indoor air ozonation for growing quails using this ozonizer in a dose of 0.01m3 of ozonated air per 1m3 of air to be cleaned reduces the relative humidity by 11.40% (P<0.01), ammonia concentration by 53.48% (P<0.01), hydrogen sulfide - by 50.98% (P<0.01), dust - by 59.18% (P<0.01), carbon dioxide - by 26.68% (P<0.05). Consequently, the daily three-hour sessions of ozonation promoted optimization of the main parameters of the microclimate in the room for the cultivation of young quail. The experiments showed that at 56 days of the experiment the use of feed additive "Basulifor" in the diet of the experimental quails in dose of 0.2 g/kg feed increased the average daily weight gain for 5.32% (P<0.01), at dose of 0.3 g/kg of feed the values were higher for 6.14% (P<0.01), the livability of young birds – for 4.32% (P<0.05) in comparison with the control counterparts.

   
Keywords: ozonizer, air ozonation, quail, humidity, ammonia, hydrogen sulfide, carbon dioxide, probiotic, feed additive, Basulifor, weight gain.
   
References:
1. Alekseev, I.A. Novaya probioticheskaya kormovaya dobavka "Basulifor" i ego vliyaniye na organizm i produktivnosty molodnyaka sviney [New probiotic feed additive "Basulifor" and its effect on body and the productivity of young pigs] / I.A.Alekseev, M.A. Pavlov, G.N.Martynov // Proceedings of Int. sci. and pract. conf. dedicated to 85th anniversary of Chuvash state agricultural academy, Cheboksary, 2016 –P. 258-262.
2. Afanasyev, G.D. Porody i raznovidnost perepelov [Breed and species of quail] / G.D.Afanasyev // Ptitsevodstvo.-1991.-Vol.3. - P.12-15.
3. Balyasnikov, V.E. Ustanovka ozonovogo obezzarazhivaniya vozduha Pozitron-3 Air 1 [Installing Pozitron-3 Air 1 Ozone for air disinfection] / V.E.Balyasnikov. - Moscow: PK “POZITRON”, 2009. – 5 p.
4. Guschin, V. Perepelovodstvo dolzhno razvivatsya [Quail breeding should develop] / V.Guschin, L.Kroik // Ptitcevodstvo.-2003. - Vol.6.- P.22-23.
5. Dedyushina, A.S. Ozon - vozmozhnosti prakticheskogo primeneniya ozona [Ozone is an opportunity of practical application of ozone] / A.S.Dedyushina. - Moscow, 2010. – 57 p.
6. Dmitriev, M.T. Gigienicheskaya otsenka ozonirovaniya vozduha [Hygienic assessment of air ozonation] / M.T.Dmitriev, Yu.L.Spiridonov, A.P.Muralov // Gigiena i sanitariya. - Moscow., 2005. - Vol.2. - P.12-14.
7. Kornilova, O.A. Vyraschivanie perepelyat [Quail breeding] / O.A.Kornilova, N.V.Kadenkova // Praktikum.-2009. - Vol.6. - P.21-23.
8. Kochetova Z.I. Novye tehnologicheskie priyomy i parametry vyraschivaniya i soderzhaniya perepelov [New ptechnological methods and parameters of quail breeding] / Z.I.Kochetova, L.S.Belyakova // Nauka – ptitsevodstvu Ivanovskoy oblasti - Implementation of scientific achievement sinto poultry breeding in Ivanoskaya oblast: proceedings from scientific and practical conference.-Sergeev Posad: izdatelystvo, 2002.- P.147-151.
9. Nastavlenie po primeneniyu probioticheskoy kormovoy dobavki «Basulifor» [Aguideline for using probiotic feed additive "Basulifor"]. - Moscow: LLC NII Probiotikov, 2011. - http:// ru/products-2basulifor_ks.
10. Stefanova, I.L. Perepelinoe yaytso i myaso v detskom pitanii [Quail egg and meat in baby food ] / I.L.Stefanova, I.A. Yuhina, M.A.Kretov // Myasnaya industriya.- 2006.- Vol.8.- P.20-22.
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  Radiobiology
   
Title:

USING THERAPEUTIC IMMUNOGLOBULIN FOR CORRECTION OF CLINICAL AND HEMATOGICAL CHANGES INDUCED BY IONIZING IRRADIATION

   
Authors: 1Tarasova N.B. – Doctor of Biological Sciences; 1Konyuhov G.V. – Doctor of Biological Sciences, professor, 1Nizamov R.N. – Doctor of Veterinary Medicine, professor; 1Vasilevski N.M. – Doctor of Veterinary Medicine, professor; 1Guryanova V.A. – Candidate of Biological Sciences; 2Yusupova G.R. – Doctor of Biological Sciences.
   
Address:
1Federal Center for Toxicological, Radiation and Biological Safety, Kazan (e-mail: vnivi@mail.ru).

2N.E.Bauman Kazan State Academy of Veterinary Medicine, Kazan (e-mail: nis_kgavm@mail.ru).

   
Abstract:
The increase in the utilization of sources of ionizing radiation increases the urgency of the problem of radiation safety, since the probability of radiation injury remains and there is a need for early laboratory diagnostics of acute radiation sickness, its forecast, treatment and protection measures. The issues of tactics and the validity of using certain preventive and therapeutic drugs closely associated with the awareness of modern ideas about the action mechanism of radioprotective compounds, their pharmacological properties and side effects. The expertise of using drugs in practice to eliminate the consequences of radiation accidents and in the areas with high radiation background, the need to use more affordable raw materials of plant and biological origin, reducing the cost of therapeutic and prophylactic agents in radiation injuries was the basis for clinical trials on laboratory and farm animals. This article describes the technology of designing radioprotective drugs and diagnostic kits based on molecular components of the immune system antibodies and antigens. The experiments were performed on a laboratory (white rats) and agricultural (sheep, calves) animals using standard techniques. The criteria for efficacy were the survival rate of irradiated animals and normalization of hematological parameters. The results showed that according to the survival rate, hematological parameters, content of products of lipid peroxidation the animals subjected to treatment after gamma irradiation had no significant differences from that of the control untreated animals. Veterinary and sanitation examination of meat from the treated sheep showed its compliance with the requirements of the State Standard norms for good meat and had the same biological values as that of the meat from intact animals. Treatment and prophylactic immunoglobulin had both local and general effects on body restoring blood parametrs, level of T-, and B-lymphocytes and reducing the number of products of lipid peroxidation, inhibiting the development of conditionally pathogenic microflora, which is the main factor in the reduction of morbidity and increase the safety of the animals.

   
Keywords: animals, radiation sickness, survival, treatment
   
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