Archive

№ 4, 2012

	Infectious pathology

Title:	FEDERAL STATE EDUCATIONAL ESTABLISHMENT HIGH PROFESSIONAL EDUCATION SARATOV STATE AGRARIAN UNIVERSITY NAMED AFTER N.I. VAVILOV

Authors:	Akchurin S.V.; Larionov S.V.

Address:	Saratov State Agricultural University in honor of N.I.Vavilov.

Abstract:	The aim of the research is estimation of functional conditions of cells of mucosa and serosal cuticle in glandular stomach part in chickens experimentally infected with colibacillosis, using method of luminescent spectral analysis and elaboration of differential and diagnostic criteria for identification of colibacillosis at the early stages of its progress. Experimental infection of chickens was made when they were two days old using field strain of bacteria Escherichia coli. Histologic specimens in glandular stomach part in baseline chickens, stained by spirit «Steins all» , were examined at the second day of life and at 1, 2, 4, 5 and 6 days after infection. Luminescence intensity value was registered in blue and red spectral range and proportion factors of nucleic acids and proteins were determined according to obtained data in accordance with the developed methodology. Using the method of luminescent spectral analysis, the way of factors determination of proportion of the nucleic acid and proteins in surface epithelium cells, interlobular connective tissue, alveolar glands epithelium and serosal cuticle in glandular stomach part in chickens the change of functional activity above mentioned cells at the various periods of life and disease in chickens` baseline was determined. Proportion factors dynamic of nucleic acids and proteins in chickens’ baseline is within the even scale-up value of indicators by their age increasing that testifies to gradual increasing of cells` functional activity. The curve reflecting dynamics of proportion factors of the chickens in baseline includes increase and decrease in their values that reveals the changes of cells functional condition. Differential and diagnostic criteria were worked out for identifying colibacillosis at the early stages using data of nucleic acid and proteins` proportion. Resulting data can be used at the development of fundamentally new approach to the problem of creation of modern diagnostic technologies, preventive measures and treatment of poultry infected with colibacillosis.

Keywords:	nucleinic acids, proteins, колибактериоз, microspectral analisis, chickens



Title:	ANTIBACTERIAL ACTIVITY OF PINE TAR SUSPENSION IN FORM OF ULTRASONIC AEROSOL

Authors:	1. Bambura M.V., 2Braslavetc V.R., 4Korjevenko G.N., 1Stupin A.Yu., 3Chubatova O.I.

Address:	1. Public corporation «GosNIISintezbelok», Russia, 109004, Moscow, 27 Alexander Solzhenitsyn street. E-mail:Mariya-bambura@yandex.ru. 2. Ltd «Inter-regional public institute of bio-resources protection», Russia, 141070, Moscow region, Korolev city, 14/16 Tsiolkovsky street. 3. Ltd «REBION» Russia, 117186, Moscow, 10 Remizov street. 4. All-Russia Scientific Research Institute of Veterinary Sanitary, Hygiene and Ecology., Russia, 123022, Moscow, 5, Zvenigorodskoe highway.

Abstract:

Keywords:	pine tar, ultrasound, suspension, aerosol, antimicrobial activity



Title:	RIFT VALLEY FEVER (EPIZOOTOLOGY, DIAGNOSTICS, PROPHYLAXIS AND CONTROL MEASURES) (SURVEY)

Authors:	N.I. Zakutsky; A.V. Knieze; V.I. Baluisheva; Gouzalova.

Address:	All Russia Research Institute for Veterinary Virology and Microbiology.

Abstract:	Characteristic of RVF agent is given, historico-geographical analysis of the RFV epizootic situation worldwide within the recent 25 years is made, and some points of epizootology, diagnostics and prevention measures for this infection are discussed.

Keywords:	Rift valley fever, epizootic, infection, methods, antibody



Title:	STUDY EMBRYOTOXIC AND TERATOGENIC PROPERTIES OF A NEW TUBERCULOSTATIC DRUG – LINAROL – F1 ON ALBINO RATS

Authors:	1. Mingaleev D.N., 2. Fattahov S.G., 2. Shulaeva M.M., 3. Valiev R.Sh., 4. Tremasov M.Ya.

Address:	1. Kazan State Academy of Veterinary Medicine named after Bauman N.E. 2. Institute Organic and Physical Chemistry of the name Arbuzova A.E. 3. Kazan State Medical Academy. 4. Federal Center of Toxicological, Radiation and Biological Safety, Kazan.

Abstract:	Searching for and making the new pharmaceuticalses, possessing tuberculostatic characteristic and allowing raise the effect an chemoprofilactic and chemotherapies of the tuberculosis remains the more actual problem. In connection with foregoing, in Institute organic and physical chemistry im. A.E. Arbuzova synthesized new tuberculostatic preparation - LINAROL-F1, which can be used as tuberculostatic of the facility in veterinary and medical practical person for preventive maintenance and treatments of the tuberculosis. The purpose of our studies was shown estimation embryotoxic and teratogenic actions new tuberculostatic preparation LINAROL-F1 with the result that it is installed that new tuberculostatic preparation - LINAROL-F1, introduced intragastral, in dose 1/10 from greatly introduced (400 mg/kgs masses of the body), during the whole pregnancy white rat, does not possess the embryotoxic and teratogenic action.

Keywords:	embryotoxic and teratogenic properties, LINAROL-F1,, new tuberculostatic drug



Title:	BASES OF PREPARATION OF HYPERIMMUNE WHEY AGAINST LEPTOSPIROSIS DOGS

Authors:	1. Verevkina M.N.; 2Surmilo A.P.

Address:	1. Тhe Stavropol State Agrarian University. 2. Тhe Federal State Unitary Enterprise «Stavropol biofactory».

Abstract:	Leptospirosis damages considerably to a national economy and constantly threatens health and human life. Depending on ways of diagnostics, the region incidence the leptospirozy is registered from exceptional and sporadic cases and reaches high as a whole about the country. Danger of infection of the person exotic serovariant leptospire raises. There is a necessity in additional studying and improvement of many scientific questions and practical importance, such as searches of chemotherapeutic and biological preparations, highly effective at treatment of clinically expressed forms of лептоспироза and the carrier of leptospirosis, the perfection of methods of manufacturing, control and application of the hyperimmune whey against leptospirosis а of dogs.

Keywords:	leptospirosis, hyperimmunization, immunity, whey, producers, areactivity



Title:	EPIZOOTOLOGICAL MONITORING OF INFLUENZA A SUBTYPES H1 AND H5 IN THE POPULATIONS OF WATERFOWL SUBFAMILY ANSERINAE WESTERN TAIMYR DURING 2005-2011

Authors:	1. Prokudin A.V., 1. Laishev K.A., 2. Sharshov K.A., 2. Durymanov A.G., 2. Shestopalov A.M.

Address:	1. State Scientific Institution Research Institute of Agriculture of the Far North RAAS. 2. State Research Center of Virology and Biotechnology «VECTOR».

Abstract:	Taimyr Peninsula is one of the most important points of the globe for conducting epizootiological observations of the state population of wild birds for many infectious diseases. This is due to the fact that nest on the peninsula of the numerous migratory birds in the Russian Federation. In the 2005-2011 years. during the spring and fall migration, as well as during the breeding season in the Western Taimyr conducted a study on the epizootic situation of influenza A in a population of waterfowl subfamily Anserinae. As a result of virological studies influenza A virus in cloacal samples from birds in the study group was not found. Studies of blood serum with the antigen of the influenza virus H1 subtype H5 have shown that antibodies to these variants of the virus found in 96-100% of the birds. This indicates that the birds which nest in the summer on the Taimyr Peninsula, is actively involved in the circulation of influenza virus and can participate in its distribution in Eurasia. Of interest is the monitoring of influenza A virus and antibodies to it, depending on the latitude of the habitat of various species of birds from southern latitudes (wintering) to the north (nesting).

Keywords:	influenza A subtypes of influenza virus, bird migration, epizootological monitoring, geese, Taimyr, antihemagglutination antibody



Title:	THE RELEVANCE OF DEVELOPMENT OF TECHNOLOGY OF PRODUCTION OF PROBIOICS VETERINARY

Authors:	Men'shenin V.V.

Address:	Russian Academy for Agricultural Sciences All-Russia Research Technological Institute for Biological Industry.

Abstract:	Currently, the practice of veterinary medicine demands for high-performance preparations of domestic production for treatment and prevention of animal and bird diseases. Production of probiotic preparations can be made only with a high content of mature and active forms of probiotic microorganisms and should include an optimal number of stages in the process of production, with adverse effects on the viability and biological properties of bacterias contained in the preparation. Researches to develop and improve the technologies of probiotics for veterinary use are important for agricultural and biological industries. Production quality is achieved through the use of the latest advances in biotechnology, its reproducibility and standardization, allows to obtain stable preparations with high efficiency during the specified periods of storage and use.

Keywords:	probiotics, dairy-sour bacteria, biotechnology of manufacture, viability of bacteria, gastroenteric diseases

	Non-transmittable diseases

Title:	DERIVATION OF MESENCHYMAL STEM CELLS FROM BONE MARROW FOR VETERINARY MEDECINE

Authors:	1. Plotnikova E.M.; 1Kirillova J.M.; 1. Khamzina E.Yu.; 2. Sokolov M.N., 1. Glagoleva I.S.

Address:	1. Federal Center of Toxicological, Radiation and Biological Safety, Kazan. 2. All Russia Research Institute of Experimental Veterinary Medicine.

Abstract:	Stem cells from adult animals were produced using Balb/c white mice. The mice were euthanized using ether anesthesia. In sterile conditions mice tibia was removed and marrow was streamed out using saline solution and syringe with needle of 22-23 g and Disaggregation of marrow from small rodents tibia was performed using mechanic handling with a needle of 22-23 g with the subsequent washing at double consecutive centrifugation (200 g, 10 mines) in 5 ml normal saline solution free from Ca2} and Mg2t and further cell plating into growth media (Sanches-Ramos J., Song S. et. al., 2000; Kruglyakov P.V.et al., 2004). Marrow purification was performed using selective adhesion and cell subsequent proliferation in cultivation conditions (in growth media). All the media were introduced glutamine (2mM), antibiotics (Penicillin and streptomycin mixture 100 mkg/ml each). Optimal pH for cell growth was from 7, 2 to 7, 4 (media alkalization should be avoided); CO2 concentration is 5% in incubator. The investigation resulted in production of mesenchyme stem cell from Balb/c mice marrow. The obtained mice marrow cells were cultivated for two weeks, however, their further in-vitro maintenance was not useful because their frequent passages promote cell ageing and losing their capability to target differentiation.

Keywords:	stem cell, mesenchymal, bone morrow stroma, osteoblast



Title:	APPLICATION PREPARATION "ILMETIN" IN THE GASTRO-INTESTINAL DISIASES

Authors:	1. Fazulzynov I.R.; 1Aglyamov, R. N.; 2. Kharitonov M. B., 3. Nurtdinov M.G.

Address:	1. Federal Center of Toxicological, Radiation and Biological Safety, Kazan. 2. Kazan State Academy of Veterinary Medicine, Kazan. 3. The Republic Tatarstan Ministry of Agriculture, Kazan.

Abstract:	The data on the efficacy of drugs "Ilmetin" and "Enterosporin" in the gastro-intestinal diseases of newborn calves. The drug "Ilmetin" is a decoction of the bark of the American Vyazya (elm), which contains tannins, flavonoids, a large number of acids, sugars, the bark of this tree contain 50-54% lignin and 41-46% fat. By the principle of analogies formed four groups of nine calves each: the first group were injected intraperitoneally with 25 ml of "Ilmetin", the second group of probiotic "Enterosporin" at a dose of 10 ml into the third group of "Ilmetin" with "Enterosporin" th in the same doses, the fourth served as a control group, this group of calves were treated according to the scheme adopted in the economy. It is established that the drug "Ilmetin" has a stimulating effect on metabolic processes, increases the safety of the calves, normalizes hematological and biochemical indices of blood. The combined use of the drug "Ilmetin" with "Enterosporinom" increases its effectiveness.

Keywords:	"Ilmetin", "Enterosporin" profuse diarrhea, levomitsitin



Title:	USE OF MSC AT HEAVY STAGES OF NEUROLOGIC FRUSTRATION IN CASE OF A HERNIA OF A DISK AT DOGS

Authors:	Kozlov N.A.

Address:	Moscow State Veterinary and Biotechnology Academy named after K.I.Skryabin.

Abstract:	The article is devoted of application mesenchymal stem cells for treatment the dogs with serious stages of herniation of the disk. There are technique of a capture of a material and preparation of cells is described. The article is devoted usage of mesenchymal allogeneic stem cells. Currently, the use of stem cells is one of the most promising directions in human medicine and animal species. Thre are not describe methods for improving stage in dogs with severe stages of the neurological deficit. The solution of this problem and has been for this study. In the experimental group had 12 dogs with a hernia in the Th12-L2, the control group (12 dogs) were the animals who, for one reason or another did not enter the cell. This article describes a method of taking material from the proximal epiphysis of the humerus, the preparation of cells. For a sampling of material used for the Jamshidi needle biopsy from the bone marrow. As the route of administration used intravenous and epidural routes of administration. Doses were administered cells - 500,000 cells / kg – i/v and 500,000 cells / kg epidural at the level of L7-S1. The introduction of the cells was carried out twice at an interval of 3 weeks. With this method indicated the absence of complications and side effects. Compared with the control group, in which the percentage of recovery was gait ability - 7% in the group with the use of mesenchymal stem cells gait was restored in 40% of dogs. The observation period - 6 months.

Keywords:	dogs disk herniation, stem cells, spine compression



Title:	FLORON'S USE AT NONSPECIFIC BRONCHOPNEUMONIA OF CALFS

Authors:	Nikulina N.B., Aksionova V.M.

Address:	Perm State Agricultural Academy, Perm.

Abstract:	We have studied a therapeutic efficiency of use of a preparation of "Floron" in treatment of the calfs sick with a nonspecific bronchopneumonia. Occurrence of disease in calf was accompanied the increase in number of leukocytes, fabric macrophages (monocytes) and unripe neutrophilis in blood testifies about to active reproduction of development of inflammatory process. For 6-7th day after the second injection florony fixed liquidation of the expressed respiratory signs at all animals which proved to be true normalization hematological and immunological indicators at calfs.

Keywords:	bronchopneumonia, calves, floron, vitam, haemostage

	Zootechny

Title:	THE LEVEL OF HEALTH AND ECONOMIC USEFULNESS ARE THE MAIN EPIZOOTIC PARAMETRS OF BIRDS' POPULATIION IN FARMS WITH VARIOUS TECHNOLOGIES OF KEEPING BIRDS

Authors:	1. Shilkina L.V., 3. Kolobov E.A., 1. Piuncina V.V., 1. Kozyrenko O.V., 3. Ibragimov Sh.N., 1. Е.H. Daugalieva, 1. Sochnev V.V.

Address:	1. Federal State Educational Establishment High Professional Education Nizhniy Novgorod State Agricultural Academy. 2. Management of veterinary medicine of West region Kazakhstan republic. 3. Committee of state veterinary control of Nizhniy Novgorod region.

Abstract:	It is installed, that level of health and economic usefulness (productivity) of birds’ population are formed under many-sided economic and technical influence. They are the main epizootic parameters of the populations.

Keywords:	populations, birds, level of health, economic usefulness, technologies of keeping poultries




Authors:	Andreev R.Yu.

Address:	All Russia Research Institute for Veterinary.

Abstract:	There are the results of researches characterizing changes of sanitary-bacteriological indicators of soil, polluted by a waste of animal industries depending on a kind of agricultural crops in this article.

Keywords:	the animal industries waste, sanitary-bacteriological indicators, agricultural crops



Title:	THE RELATIONSHIP OF BIOCHEMICAL PARAMETERS OF BLOOD FROM THE MEAT PRODUCTIVITY OF BULL FATTENING OF DIFFERENT GENOTYPES

Authors:	1. Hazipov N.N., 2. Bikchantaev I.T., 3. Afanasiev A.P.

Address:	1. Ministry of Agriculture and Food of the Republic of Tatarstan. 2. Tatar State Scientific Institution Research Institute Rosselhozakeademii, Kazan. 3. Kazan State Academy of Veterinary Medicine, Kazan.

Abstract:	Conducted a study on the efficiency of high-quality beef at a dilution of the meat industry and the limousine breed crosses of this breed with black and white cattle.The article presents research results of biochemical blood parameters and meat efficiency of fattening bull different genotypes. Studies have shown the possibility and highly cost-effective production and feeding limousin and crossbred calves produced by mating with a black and white and limousin rocks, which is especially suitable for areas of intensive livestock farming and fodder production.

Keywords:	feeding, limousine, hybrid, blood, slaughter, production, beef, economy



Title:	EXPERIENCE OF APPLICATION OF THE PROBIOTIC ADDITIVE TO THE FORAGE «BATSELL», AT CULTIVATION OF YOUNG GROWTH QUAIL

Authors:	Kirillov N.K.; Alekseyev I.A.; Ivanova R.N.

Address:	Chuvash State Agricultural Academy.

Abstract:	The purpose of our work was determination of zootechnical, veterinary and economic feasibility of application of a probiotic «Batsell» at cultivation of quail. Researches are carried out on JSC «Vurnarskaya incubator-poultry-farming station» of the Chuvash Republic in 2010-2011. The young growth of a bird of breed the Pharaoh of daily age was object of research. In skilled group to a forage of birds added 0,2 % of a probiotic from weight of dry compound feed within 40 days. Researches showed that in skilled group of birds for the 14th days bactericidal activity of blood was higher for 4,74 % in comparison with control group, lizotsimny and fagotsitarny – 5,23 and 4,81 % (Р<0,05), for the 28th days - 5,34, 6,11, 6,36 % (Р<0,05-0,01), for the 56th days - 6,12, 6,98, 6,93 % (Р<0,01) respectively. The most intensive growth was observed at birds of skilled group on 21, 35, 42, 49, the 56th days for 3,71-5,53 % (Р<0,05-0,01) in comparison with control group. In control group of birds safety made 95,0 %, and in skilled group - 97,5 % (Р<0,01). Economic efficiency on 1 ruble of expenses made 3,6 ruble. Thus, for the purpose of activization of nonspecific resistance, increase of meat efficiency at young growth of birds, and also increases of their safety we recommend to use a pro-biotic preparation «Batsell».

Keywords:	probiotic supplement to the food "Batsell", growth and development of quail, daily gain; live weight, safety



Title:	MORPHOLOGICAL AND PHYSICO-CHEMICAL INDICATORS OF BLOOD OF THE COWS WHEN USING BIOLOGICALLY ACTIVE ADDITIVE “GUMIFIT” IN THEIR DIET

Authors:	Frolov A.V.

Address:	Russian state trading and economy university, Kazan institute.

Abstract:	We were studying morphological and physico-chemical indicators of cows blood when using biologically active food additive “Gumifit” in their diet. Into the diet of the cows of the experimental group the preparation “Gumifit” was included during all the period (7 months – stable period) 0,15 ml/kg of weight every day. The composition of additives include biological active compounds of the complex humic (humic acids, fulvic acids, melanoiidiny), amino acids (lysine, methionine, proline, leucine, threonine, serine, etc.), low molecular weight carboxylic acids (oxalic, succinic, salicylic, cinnamic, etc. ), carbohydrates (D-glucose, D-galactose, xylose, mannose, etc.), macro-and microelements (calcium, phosphorus, magnesium, iron, silicon, aluminum, copper, selenium, cobalt, iodine, etc.). The increase in hemoglobin in the blood of experimental cows compared to controls was 6-7.5%, red blood cells - 5,5-9,2%, white blood cells - 3.3 - 5.1%. In terms of leukocyte counts of deviations from the physiological groups were observed. The content of total protein in the blood serum of cows fed diets containing biologically active supplement "Gumifit" within seven months of the experiment was in the range 82,3 ± 0,6 - 85,6 ± 0,6 g / l. In control animals, the figure was 78,9 ± 0,2 - 85,1 ± 0,5 g / l. A significant increase of total protein in the blood serum of experimental cows were observed in the third month of experience, continued until the end of the study and was 1.5 - 7.3%. It was also an increase in gamma-globulins in the 2,4-10,2%. Change the content of albumin, alpha-and beta-globulins were less pronounced. The content of glucose in the blood of experimental cows during seven months of research ranged from 2,86 ± 0,04 to 3,03 ± 0,01 mmol / l in the blood of control animals, 2,67 ± 0,02 - 2,85 ± 0 , 03 mmol / l. The excess of the rate in experience over the control sostovlyaet 2,1-9,4%. With the introduction of the diet of cows feeding the drug "Gumifit" calcium in the blood during the experiment was in the range 2,68 ± 0,03 -2,82 ± 0,03 mmol / l in the control animals this figure was 2,66 ± 0 , 03 -2,75 ± 0,04 mmol / l.Prevyshenie of calcium in the blood of experimental control over the cow was 1,5-5,2% The content of inorganic phosphorus in the peripheral blood of cows, diets containing the drug "Gumifit" more than the same period by 1.2 -8.7%. Consequently, the use of the diet of lactating cows during the stabling period of biologically active food supplement "Gumifit" a daily dose of 0.15 ml / kg body weight contributes to sustained stimulation of hematopoiesis, protein, carbohydrate and mineral metabolism.

Keywords:	cows, food additives, blood indicators

	Radiobiology

Title:	FEATURES OF THE ESERIHIOZNOGO PROCESS IN MICE UNDER CONDITIONS OF OXIDATIVE STRESS DUE TO RADIATION EXPOSURE

Authors:	Gayzatullin R.R.

Address:	Federal Center of Toxicological, Radiation and Biological Safety, Kazan.

Abstract:	The study of features of flow of Escherichia of infectious process is conducted in the conditions of affecting organism of ionizing radiation and modification of flow of illness on a background application of polyfunctional preparation of "Poliapisogen". It is set that the flow of Escherichia infection on a background radiogenic stress is characterized by aggravating of infectious process, attended with insufficiency of the system of the antioxidant defence, conditioned by the uncompensated operating on the cellular membrane of toxic foods of peroxide oxidization of lipids, defiant hypofunction of organs of immunogemopoeza, promoting, the same, to the increase of activity opportunistic bacterial endo- and ekzomikroflory, resulting in development septic form of Escherichia infection. Single hypodermic introduction to the white mise of preparation of "Poliapisogen" both to (after 24 ч) and after(through 24 ч) an infection the causative agent of Escherichia and subsequent gamma irradiation rendered the modifying operating on a flow and end of Escherichia, providing 75-80 % survival rate for the infected and radiation-exposed animals.

Keywords:	eserihioznay infection of mice exposed to oxidative stress, antioxidant protection

	Physiology

Title:	MORPHOGENEZGONOD OF THE YAK IN EMBRYO AND PREBREED PERIOD OF DEVELOPMENT

Authors:	Zamyanov I.D.

Address:	The Buryat state Academy of Agriculture named after V.R.Philippov.

Abstract:	Domestic yaks are adapted to survival in harsh climates, undemanding in terms of keeping and feeding, can use the pastures inaccessible to other types of pets, which allows minimal material cost to get an inexpensive clean and high quality products. The material for the study were the gonads of embryos from 3 and 3 prefetal yak. The fence material is produced on Kultukskom meat factory, the material was fixed in 10% neutral formalin solution. Age of embryos and prefetal determined by worksheet Vasiliev KA In embryos yak 20-23 day-old embryo gonads are valikovidnoy forms on the ventral surface of the mesonephros. Epithelial cells in the gonad rudiment composed of cuboidal epithelium, mesenchymal cells form the basis otrostchatoy gonads. Intercellular substance is eosinophilic stain. Blood vessels are lined with flat endothelial cells, and their lumen are found rounded cells with oxyphilic cytoplasm and round nuclei - erythroblasts. Mesonephros tubules lined by cuboidal epithelium. Mesonephros tubules surrounded mezenhimotsitami. In 25-30 day-old embryos in the medial surface of the mesonephros are formed by the gonads. The basis of the gonads is the mesenchyme, but outside they are covered by coelomic epithelium. The vessels are the vessels of the mesenchyme basis sinusoidal type. In the mesenchyme of the gonad appear gonocytes. In the pre yak 35-37 day-old gonads differentiate into testes side. Under the surface epithelium of the tunica albuginea is formed, and in the mesenchyme on the epithelial gonadal cords appear.

Keywords:	embryo, prebreedmezophros, gonada, mezenchima, epiteliy

The journal is registered

Decision of the Presidium

Archive

№ 4, 2012